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Regulatory mechanism of Angelica Gigas extract powder on matrix metalloproteinases in vitro and in vivo model
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  • Journal title : Analytical Science and Technology
  • Volume 28, Issue 6,  2015, pp.361-369
  • Publisher : The Korean Society of Analytical Science
  • DOI : 10.5806/AST.2015.28.6.361
 Title & Authors
Regulatory mechanism of Angelica Gigas extract powder on matrix metalloproteinases in vitro and in vivo model
Kwon, Jin-Hwan; Han, Min-Seok; Lee, Yong-Moon;
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 Abstract
The precise mechanism underlying the therapeutic efficacy of an extraction powder of Angelica gigas (AGE) for the treatment of degenerative osteoarthritis was investigated in primary cultured rabbit chondrocytes and in a monosodium-iodoacetate (MIA)-induced osteoarthritis rat model. The treatment with AGE (50 μg/mL) effectively inhibited NF-B activation. The anti-inflammatory mechanism was clarified by gelatin zymography and western blotting measurements of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities. The AGE (50 μg/mL) treatment significantly reduced MMP-9 activity. The constituents of AGE— decursinol, decursin, and decursinol angelate—were determined by LC-MS/MS after a 24 hr treatment of rabbit chondrocytes. The contents of the major products, decursin and decursinol angelate, were 3.62±0.47 and 2.14 ±0.36 μg/mg protein, respectively in AGE-treated (50 μg/mL) rabbit chondrocytes. An in vivo animal study on rats fed a diet containing 25, 50, and 100 mg/kg AGE for 3 weeks revealed a significant inhibition of the MMPs in the MIA-induced rat articular cartilage. The genetic expression of arthritic factors in the articular cartilage was examined by RT-PCR of collagen Type I, collagen Type II, aggrecan, and MMP (MMP3, MMP-9, MMP13). Specifically, AGE up-regulated the expression of collagen Type I, collagen Type II, and aggrecan and inhibited MMP levels at all tested concentrations. Collectively, AGE showed a strong specific site of action on MMP regulation and protected against the degeneration of articular cartilage via cellular regulation of MMP expression both in vitro and in vivo.
 Keywords
Angelica gigas;osteoarthritis;MMPs;Decursin;Decursinol angelate;ectract;
 Language
Korean
 Cited by
 References
1.
D. Felson, Epidemiol Rev., 10, 1-28 (1988).

2.
V. B. Kraus, Med. Clinics North Am., 81, 85-112 (1997). crossref(new window)

3.
J. Martel-Pelletier, R. McCollum, N. Fujimoto, K. Obata, J. M. Cloutier and J. P. Pelletier, Lab Invest, 70, 807-15 (1994).

4.
J. F. Jr, Woessner and Z. Gunja-Smith, J. Rheumatol, 27(Suppl), 99-101 (1991).

5.
D. D. Dean, J. Martel-Pelletier, J. P. Pelletier, D. S. Howell and J. F. Jr. Woessner, J. Clin. Invest., 84, 678-685 (1989). crossref(new window)

6.
S. Roy, S. Khanna, A. V. Krishnaraju, G. V. Subbaraju, T. Yasmin, D. Bagchi and C. K Sen, Antioxid Redox Sign, 8, 653-660 (2006). crossref(new window)

7.
B. Park, S. Prasad, V. Yadav, B. Sung and B. B. Aggarwal, PLoS One, 6, e26943 (2011). crossref(new window)

8.
K. Sengupta, J. N. Kolla, A. V. Krishnaraju, N. Yalamanchili, C. V. Rao, T. Golakoti, S. Raychaudhuri and S. P. Raychaudhuri, Boswellia serrata extract. Mol Cell Biochem, 354, 189-197 (2011). crossref(new window)

9.
KHIDI. Health functional food industry development assistance report. Korea Health Industry Development Institute, Chungbuk, Korea. p 8 (2011).

10.
K. S. Ahn, W. S. Sim and I. H. Kim, Planta Med., 62(1), 7-9 (1996). crossref(new window)

11.
K. S. Ahn, W. S. Sim, I. K. Lee, Y. B. Seu and I. H. Kim, Planta Med., 63(4), 360-361 (1996).

12.
Ammon HRT. Phytomedicine, 17, 862-867 (2010). crossref(new window)