Publisher : The Korean Society of Analytical Science
DOI : 10.5806/AST.2015.28.6.425
Title & Authors
Development of a cholesterol biosensor modified with carbon nanotube Kim, Haidong;
A cholesterol biosensor was developed using a modified carbon electrode with carbon nanotubes. The disposable cholesterol biosensor was modified with carbon nanotubes to enhance electron transfer during the enzymatic reaction of cholesterol. Cholesterol oxidase and peroxidase, with potassium ferrocyanide as a mediator, were immobilized on a screen-printed carbon nanotube electrode. The electrochemical cholesterol biosensor developed using carbon nanotubes showed a rapid and reliable signal for measuring total cholesterol. The cholesterol sensor showed a linear response in 5 seconds with a small volume (0.5 μL) in the range of 100~400 mg/dL, with a coefficient of variation of 4.0%.
S. K. Arya, M. Datta and B. D. Malhotra, Biosens. Bioelectron., 23, 1083-1100 (2008).
G. R. Warnick, M. M. Kimberly, P. P. Waymack and E. T. Leary, Labmedicine, 39(8), 481-490 (2008).
O. Turkarslan, S. K. Kayahan and L. Toppare, Sens. Actuators B, 136, 484-488 (2009).
J. B. Claver, J. A. Cabello, J. M. Sanfrutos, A. M. Fernandez, M. C. V. Miron, F. S. Gonzalez and L. F. C. Vallvey, Anal. Chim. Acta, 754, 91-98 (2012).
C. K Pires, B. F. Reis, C. X. Galhardo and P. B. Martelli, Anal. Lett., 36, 3011-3024 (2003).
B. Canabate-Diaz, C. A. Segura, A. F. Gutierrez, V. A. Belmonte, F. A. Garrido, V. J. L. Martinez and M. J. Duran, Food Chem, 102, 593-598 (2007).
Y. Y. Hosokawa, H. Hakamata, T. Murakami, S. Aoyagi, M. Kuroda, Y. Mimaki, Y. A. Ito, S. Morosawa and F. Kusu, Electrochim. Acta, 54, 6412-6416 (2009)
M. Barbadillo, E. Casero, Petit-Dominguez, M. D., L. Vazquez, F. Pariente and E. Lorenzo, Talanta, 80, 797-802 (2009).
Y. Wang, X. Hui, J. Zhang and L. Guang, Sensors, 8, 2043-2081 (2008).
S. Park, H. Boo and T. D. Chung, Anal. Chim. Acta, 556, 46-57 (2006).
F. Ricci and G. Palleschi, Biosens. Bioelectron, 21, 389-407 (2005).
J. L. Yi, D. K. Jung and Y. P. Jae, J. Kor. Phys. Soc., 54, 1769-1773 (2009).
H. Chengguo and H. Shenghui, J. Sensors, 1-40 (2009).
J. Wang, Electroanal., 17, 7-14 (2005).
M. Q. Israr1, K. Hasan, J. R. Sadaf, I. Engquist, O. Nur, M. Willander and B. Danielsson, J. Biosens Bioelectron, 2(3), 1000109 (2011).