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Performance of MiniPCRTM mini8, a portable thermal cycler
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  • Journal title : Analytical Science and Technology
  • Volume 29, Issue 2,  2016, pp.79-84
  • Publisher : The Korean Society of Analytical Science
  • DOI : 10.5806/AST.2016.29.2.79
 Title & Authors
Performance of MiniPCRTM mini8, a portable thermal cycler
Kwon, Han-Sol; Park, Hyun-Chul; Lee, Kyungmyung; An, Sanghyun; Oh, Yu-Li; Ahn, Eu-Ree; Jung, Ju Yeon; Lim, Si-Keun;
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A small and inexpensive thermal cycler (PCR machine), known as the MiniPCRTM Mini8 Thermal Cycler (Amplyus, Cambridge, MA, USA), was developed. In this study, the performance of this PCR machine was compared with the GeneAmp® PCR system 9700 (Applied Biosystems) using four autosomal short tandem repeat (STR) kits, a Y-chromosome STR kit, and a mitochondrial DNA HV1/HV2 sequence analysis. The sensitivity and stochastic effects of the STR multiplex kits and the quality of the DNA sequence analysis were similar between the two PCR machines. The MiniPCRTM Mini8 Thermal Cycler could be used for analyses at forensic DNA laboratories and crime scenes. The cost of the PCR is so economical that school laboratories and individuals could use the machines.
MiniPCRTM mini8 Thermal Cycler;GeneAmp®PCR system 9700;performance;STR multiplex kits;mtDNA HV1/HV2;
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Simple and rapid identification of saliva by detection of oral streptococci using direct polymerase chain reaction combined with an immunochromatographic strip, Forensic Science International: Genetics, 2018, 33, 155  crossref(new windwow)
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