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Effect of functional group on activity and stability of lipase immobilized on silica-coated magnetite nanoparticles with different functional group
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  • Journal title : Analytical Science and Technology
  • Volume 29, Issue 3,  2016, pp.105-113
  • Publisher : The Korean Society of Analytical Science
  • DOI : 10.5806/AST.2016.29.3.105
 Title & Authors
Effect of functional group on activity and stability of lipase immobilized on silica-coated magnetite nanoparticles with different functional group
Lee, Hye Rin; Kim, Moon Il; Hong, Sang Eun; Choi, Jaeyeong; Kim, Young Min; Yoon, Kuk Ro; Lee, Seungho; Ha, Sung Ho;
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 Abstract
The present study investigated the immobilization of lipases on silica nanoparticles and silica-coated magnetite nanoparticles as supports with a functional group to enhance the stability of lipase. The influence of functional groups, such as the epoxy group and the amine group, on the activity and stability of immobilized lipase was also studied. The epoxy group and the amino group were introduced onto the surface of nanoparticles by glycidyl methacrylate and aminopropyl triethoxysilane, respectively. Immobilized Candida rugosa lipase on silica nanoparticles and silica-coated magnetite nanoparticles with a functional group showed slightly lower initial enzyme activities than free enzyme; however, the immobilized Candida rugosa lipase retained over 92 % of the initial activity, even after 3 times reuse. Lipase was also immobilized on the silica-coated magnetite nanoparticles by cross-linked enzyme aggregate (CLEA) using glutaraldehyde and covalent binding, respectively, were also studied. Immobilized Candida rugosa lipase on silica nanoparticles and silica-coated magnetite nanoparticles by CLEA and covalent binding showed higher enzyme activities than free enzyme, while immobilized Candida rugosa lipase retained over 73 % of the initial activity after 5 times reuse.
 Keywords
nanoparticle;silica-coated magnetite nanoparticles;lipase;immobilization;activity;stability;
 Language
Korean
 Cited by
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