JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Taraxaci Herba on Cell Death in Breast Cancer Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Taraxaci Herba on Cell Death in Breast Cancer Cells
Seo, Kook-Jang; Park, Kyung-Mi; Joh, Sung-Hee; Yang, Seung-Jeong;
  PDF(new window)
 Abstract
Objectives : This study was designed to investigate the effects of Taraxaci Herba (TH) on cell death in breast cancer cells. Methods : In this experiment, the effects of TH on proliferation rates, cell morphology and growth pattern, intracellular reactive oxygen species (ROS) production. In addition, the effects on nuclear condensation, fragmentation and formation of acidic vesicular organelles (AVO) in MCF-7 cells were also investigated. Finally, autophagy related with protein was observed by using western blot method. Results : TH inhibited proliferation of MCF-7 cells, TH elevated intracellular ROS levels significantly. Treatment with TH did not affect nuclear morphologies such as condensation or fragmentation. On the other hand, TH treatment effectively induced AVO. Finally, one of autophagy related with protein, Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, LC3) level was elevated by treatment with TH. Conclusions : These data indicate that TH is able to be used for patient with breast cancer and mechanisms are involved in autophagy through ROS generation.
 Keywords
Taraxaci Herba;breast cancer cell;autophagy;
 Language
Korean
 Cited by
 References
1.
National Cancer Information Center. Cancer facts & figures. 2014. Available from:URL:http://www.cancer.go.kr/mbs/cancer/jsp/album/gallery.jsp?addCancerTitle&spage=3&boardId=31817&boardSeq=4580992&mcategoryId=&id=cancer_050207000000.

2.
Korean Society of Obstetrics and Gynecology. Gynecology(4). Seoul: Korea Medical Book Publisher Co. 2013:931-48.

3.
The Society of Korean Obstetrics and Gynecology. Korean Obstetrics and Gynecology(2). Seoul:EuiSeongDang. 2012:393-9.

4.
Han MO, et al. Body image and physical discomfort during the period of radiotherapy in breast cancer patients with breast conserving operation. Graduate School of Industry University Of Ulsan. 2014.

5.
So WB. Jaebeongwonhuron. Seoul: Daesung Publishers. 1992:296.

6.
Jin JM. Buindaejeonyangbang. Seoul: Jungdam Publishing. 1993:72.

7.
Woo D. Uihakjungjeon. Seoul:Seongbosa Publishing. 1986:310.

8.
Ju S. Bojaebang. Beijing:Beijing Publishing. 1988:1-3.

9.
Huh J. Donguibogam. Seoul:Bubin Publishers. 2007:765.

10.
Jang GB. Kyoungakjeonseo. Seoul :Hanmi Medical Publishing. 2006: 1841-2.

11.
Kim JJ, et al. A bibiographic study on the treatment of the breast mass. The Journal of Oriental Obstetrics & Gynecology. 1998;11(2):29-43.

12.
Jin SG. Oeguwajeungjong. Beijing: People’s Medical Publishing House. 1964:144-5.

13.
Nationwide College of Oriental Medicine, Habalism edited by Professor. Herbalism. Seoul:Younglim Inc. 2006:245-7.

14.
Hwang KJ, et al. A study of the anti-inflammatory effects of Taraxaci Herba. Gyeonggi-do:Graduate school of Korean Medicine Gachon University. 2011.

15.
Ham DH, et al. Anti-inflammatory activity of dandelion in animal models. The Korea Journal of Physiology & Pathology. 2008;22:810-4.

16.
Yea HK, et al. Study of immuno-modulating activities of the fractions from Taraxacum officinale. Gyeongsangnam-do:Department of Microbiology, Graduate school Inje University. 2005.

17.
Lee BH, et al. Apoptosis Induction Effect of Hepatoblastoma by Taraxacum officinale. Iksan:Graduate school of Korean medicine Wonkwang University. 2001.

18.
Sun T, et al. Effect of aconiti lateralis radix praeparata and taraxaci herba on chinese medicine signs and symptoms of urethane-induced lung cancer in mice. Zhongguo Zhong Yao Za Zhi. 2012 Oct;37(20):3097-101.

19.
Gong JH. ManByeungHweochun. Beijing: People’s Medical Publishing House. 1990:373.

20.
Ju JH. Dangyeasimbubburok. Seoul: Daesung Publishers. 1993:585-9.

21.
Ra WG, et al. Gynecology of traditional Chinese Medicine. Beijing: People’s Medical Publishing House. 1988:44-50.

22.
Sul G. Kyojubuinyangbang yeokhae. Seoul:Jungdam Publishing. 2011:798-804.

23.
Lee JH, et al. Meta Analysis of researches about herbal extracts used in breast cancer in South Korea since 2000. Daejeon University, Institue of korean medicine, Thesis Collection. 2007;16(2):241-9.

24.
Abraham MC, Shaham S. Death without caspases, caspases without death. Trends Cell Biol. 2004;14(4) :184-93. crossref(new window)

25.
Lee KJ, et al. Analysis of paper related herba of anti-cancer effects by Autophage. Daejeon University, Institue of korean medicine, Thesis Collection. 2012;20(2):91-8.

26.
Lorenzo HK, Susin SA. Therapeutic potential of AIF-mediated caspase independent programmed cell death. Drug Resist Updat. 2007;10(6):235-55. crossref(new window)

27.
Yorimitsu T, Klionsky DJ. Autophagy :molecular machinery for self-eating. Cell Death Differ. 2005;12:1542-52. crossref(new window)

28.
Bursch WA, et al. Active cell death: role in hepatocarcinogenesis and subtypes. Biochem Cell Biol. 1994;72(11-12) :669-75. crossref(new window)

29.
Dunn WA Jr. Studies on the mechanisms of autophagy: formation of the utophagic vacuole. J Cell Biol. 1990;110(6) :1923-33. crossref(new window)

30.
Scherz-Shouval R, Elazar Z. ROS, mitochondria and the regulation of autophagy. Trends Cell Biol 2007;17 :422-7. crossref(new window)

31.
Guo WJ, et al. ROS-mediated autophagy was involved in cancer cell death induced by novel copper(II) complex. Exp Toxicol Pathol. 2010;62(5):577-82. crossref(new window)

32.
Cheon SJ, et al. Study on the application of cosmetic agent and antioxidative activity of Taraxcum platycarpuma. Journal of the Korean Society of Cosmetology. 2007;13(1):293-7.

33.
Kim HW, et al. Protective effects of keratinocytes and antioxidative effects of Taraxaci Herba Extracs. The Korea Journal of Herbology. 2009;24(3):103-8.

34.
Ha YD. The anticancer, antioxidative and antimicrobial effects of Grifola umbellata Extracts. Korean Journal of Food Preservation. 2001;8(4):481-7.

35.
Lee HK, et al. The antioxidative, antimutagenic and anticancer activivity effects of Cirsium japonicum Extracts. Korean Journal of Medicinal Crop Science. 2003;11(1):53-61.

36.
Lacombe F, Belloc F. Flow cytometry study of cell cycle, apoptosis and drug resistance in acute leukemia. Hematol Cell Ther. 1996 Dec;38(6) :495-504. crossref(new window)

37.
Zakeri Z, et al. R. A. Cell death: programmed apoptosis, necrosis, or other? Cell Death Differ. 1995;2:87-96.

38.
Paglin S, et al. A novel response of cancer cells to radiation involves autophagy and formation of acidic vesicles. Cancer Res. 2001;61(2):439-44.

39.
Cherra SJ, et al. Regulation of the autophagy protein LC3 by phosphorylation. J Cell Biol. 2010;190(4):533-9. crossref(new window)

40.
He H, et al. Physalin A induces apoptosis via p53-Noxa-mediated ROS generation, and autophagy plays a protective role against apoptosis through p38-NF-κB survival pathway in A375-S2 cells. J Ethnopharmacol. 2013;148(2):544-55. crossref(new window)