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Effects of Chelidonium Majus Extract on Apoptosis Induction of MDA-MB-231 Human Breast Cancer Cells
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 Title & Authors
Effects of Chelidonium Majus Extract on Apoptosis Induction of MDA-MB-231 Human Breast Cancer Cells
Jang, Sae-Byul; Yoo, Dong-Youl;
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 Abstract
Objectives : In this study, we investigated the anti-proliferative and apoptosis inducing effect of water extract of Chelidonium majus (CM) on human breast cancer cell MDA-MB-231. Methods : The MTT assay was used to assess cell proliferation. The expression of apoptosis related gene was assessed by quantitative Real-time PCR. Cell apoptosis detected by flow cytometry using Annexin-V/PI staining. Results : Our data revealed that CM inhibited the cell growth in a dose dependent manner (0, 62.5, 125, 250, 500 μg/ml). CM increased mRNA expression of pro-apoptotic genes Bax, caspase-3, and caspase-9. Annexin-V/PI staining assays revealed that apoptosis-induced cell death increased in a dose-dependent manner in cells. Conclusions : CM induces cell death in MDA-MB-231 human breast cancer cell and shows potentials for use in cancer therapy as apoptosis-inducing agent.
 Keywords
Chelidonium majus;breast cancer;MDA-MB-231;apoptosis;anti-proliferative;
 Language
Korean
 Cited by
1.
박동관류 심방모델에서 강심효과를 나타내는 단미 한약재 검색,이윤정;권오정;김혜윰;남궁승;이재윤;유윤조;강대길;

동의생리병리학회지, 2016. vol.30. 1, pp.40-46 crossref(new window)
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