Advanced SearchSearch Tips
Effects of Insamyangyoung-tang Aqueous Extracts on the Hypothyroidism Induced by Propylthiouracil in Rats
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Insamyangyoung-tang Aqueous Extracts on the Hypothyroidism Induced by Propylthiouracil in Rats
Park, Eun-Young; Kim, Dong-Chul;
  PDF(new window)
Objectives : The object of this study was to evaluate the effect of Insamyangyoung-tang aqueous extracts (ISYYT) on the propylthiouracil (PTU) induced rat hypothyroidism. Methods : The rats were divided into 6 groups : intact control, PTU control, Levothyroxine (LT 4 ), 500, 250 and 125 mg/kg ISYYT treated groups. In ISYYT treated groups, PTU and ISYYT were administered for 4 weeks after 500, 250 and 125 mg/kg ISYYT were administered for 2 weeks. In LT 4 group, PTU and LT 4 were administered for 4 weeks. The changes were observed : the weigh, serum thyroid hormone levels, serum sex hormone levels, serum lipid profiles, serum liver enzyme levels, liver and testis antioxidant defense system, histopathology of thyroid gland, liver, epididymis, prostate and testis. Results were compared with PTU control group in this experiment. Results : In comparison with PTU control group, 500 and 250 mg/kg ISYYT treated groups showed significant increase of body, thyroid, liver, testis, epididymis and prostate weights, decrease of serum TSH levels with increase of serum T3 and T4 level, increase of serum testosterone and DHT levels with decrease of serum FSH levels, decrease of serum HDL with increase of TG and increase of serum AST levels. Histopathological inspections of hepatic and male reproductive organ damage induced by PTU were improved. And the changes of antioxidant defense system of Liver and testis induced by PTU were improve. There was no significant difference between 125 mg/kg ISYYT treated group and PTU control group in this experiment. Conclusions : The results obtained in this study considered that Insamyangyoung-tang may be effective in hypothyroidism and related organ damages.
 Cited by
Lee BY. Clinical Thyroidology. 4th. Seoul:Goryeo medicine. 2014:257-8, 341-42, 346-58, 391.

Korean Endocrine Society. Endocrinology and Metabolism. 2nd. Seoul:Korean Endocrine Society. 2011:113-6, 134, 168, 170-7.

Biondi B, Klein I. Hypothyroidism as a risk factor for cardiovascular disease. Endocrine. 2004;24(1):1-13. crossref(new window)

Subudhi U, et al. Supplementation of curcumin and vitamin E enhances oxidative stress, but restores hepatic histoarchitecture in hypothyroid rats. Life Sci. 2009;84:372-9. crossref(new window)

Das K, Chainy GB. Modulation of rat liver mitochondrial antioxidant defence system by thyroid hormone. Biochim Biophys Acta. 2001;1537(1):1-13. crossref(new window)

Wiersinga WM. Thyroid hormone replacement therapy. Horm Res. 2001; 56(1):74-81. crossref(new window)

Roberts CG, Ladenson PW. Hypothyroidism. Lancet. 2004;363(9411):793-803. crossref(new window)

Du HY. DongeuiSingyehak. Seoul:Institute of Oriental Medicine. 1993:1065.

Jo CS, Kim DB, Kim CJ. Efffects of Danggwisaeyeoktan on the Hypothyroidism of Rats. The Korea Journal of Herbology. 2007;22(1):95-102.

Choi IG, et al. Effects of Jinmutang on Hypothyroidism in Rats. The Journal of Korean Oriental Internal Medicine. 2006;27(4):879-87.

Kim DH, et al. The Effects of Daeyoungjeon on the Hypothyroidism in Rats. The Korea Journal of Herbology. 2007;22(4):35-43.

Lee SH, et al. The Effects of Aconiti Radix on Thyroid Function in Hypothyroidism Rat Model induced by 6-propyl-2-thiouracil(PTU). The Journal of Korean Oriental Internal Medicine. 2007;28(2):275-83.

Kang GH, et al. Effects of Zingiberis rhizoma on Hypothyroidism Rat induced by PTU. The Journal of Korean Oriental Internal Medicine. 2006;27(3): 677-87.

Kang CH, et al. The Effects of Evodiae fructus on Thyroid function and Histological modification in Hypothyroidism Rat Model induced by PTU(6-Propyl,2-thiouracil). The Journal of Korean Oriental Internal Medicine. 2008;29(4):1000-10.

Kim SM. Effects of Ginseng Radix on the Rat Hypothyroidism induced by PTU(6-n-propyl-2-thiouracil). The Korea Journal of Herbology. 2010; 25(3):11-8.

Kang JS. The Effects of Cuscuta semen on a Hypothyroidism Rat Model induced by Propylthiouracil (PTU). The Journal of Korean Oriental Internal Medicine. 2010;31(3):425-36.

Yang HT, et al. Study of Effect of Cervus nippon temminck aqua-acupuncture on the Hypothyroidism induced by Thiourea in Rats. Journal of Korean acupuncture & moxibustion medicine society. 1992;9(1):215-27.

Joo TC, Choi YT. Effect of Direct Moxibustion on Depressed Immune Response and Thyroid Hormone in Rats Exposed to Cold Stress. Journal of Korean acupuncture & moxibustion medicine society. 1998;15(2):157-71.

Taepyungheymin-hwajeguk. Taepyungheymin-hwajegukbang. China: The public hygiene publishing company. 1985:197-8.

Heo J. Donguibogam. Gyeongnam:Dongui Bogam publishing company. 2005:1261-2.

Mogulkoç R, et al. Influence of hypovolemic and hypertonic treatments on plasma vasopressin levels and fluid balance in the propylthiouracil- induced hypothyroid rat. Neuro Endocrinol Lett. 1999;20(5):311-4.

O’Connor JC, Frame SR, Ladics GS. Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators. Toxicol Sci. 2002; 69(1):79-91. crossref(new window)

Kavutcu M, et al. Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs: effects of vitamins E and C. Nephron. 1996;72(2):269-74. crossref(new window)

Lowry OH, et al. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.

Jamall IS, Smith JC. Effects of cadmium on glutathione peroxidase, superoxide dismutase and lipid peroxidation in the rat heart: a possible mechanism of cadmium cardiotoxicity. Toxicol Appl Pharmacol. 1985;80(1):33-42. crossref(new window)

Pick E, Keisari Y. Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages-induction by multiple nonphagocytic stimuli. Cell Immunol. 1981;59(2): 301-18. crossref(new window)

Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-6. crossref(new window)

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 1984;2:130-2.

Gartner LP, Hiatt JL. Color Textbook of Histology, 3rd ed. Philadelphia: Saunders-Elsevier. 2007:489-510.

Kim MS, et al. Changes in Plasma Leptin Levels Relating to Short-Term Thyroid Manipulation in Rats. Journal of Korean Endocrine Society. 2002; 17(2):197-205.

Duntas LH. Thyroid disease and lipids. Thyroid. 2002;12(4):287-93. crossref(new window)

Teixeira Pde F, et al. Lipid profile in different degrees of hypothyroidism and effects of levothyroxine replacement in mild thyroid failure. Transl Res. 2008;151(4):224-31. crossref(new window)

Cappola AR, Ladenson PW. Hyperthyroidism and atherosclerosis. J Clin Endocrinol Metab. 2003;88(6):2438-44. crossref(new window)

Dory L, Roheim PS. Rat plasma lipoproteins and apolipoproteins in experimental hypothyroidism. J Lipid Res. 1981;22(2):287-96.

Ji LL. Antioxidant enzyme response to exercise and aging. Med Sci Sports Exerc. 1993;25(2):225-31.

Kim YG. Antioxidants. Seoul:Yeomungak. 2004:154.

Feng X, et al. Thyroid hormone regulation of hepatic genes in vivo detected by complementary DNA microarray. Mol Endocrinol. 2000;14(7):947-55. crossref(new window)

Choudhury S, Chainy GB, Mishro MM. Experimentally induced hypo- and hyper-thyroidism influence on the antioxidant defence system in adult rat testis. Andrologia. 2003;35(3):131-40. crossref(new window)

Peltola V, Huhtaniemi I, Ahotupa M. Antioxidant enzyme activity in the maturing rat testis. J Androl. 1992; 13(5):450-5.

Sahoo DK, Roy A, Chainy GB. Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chem Biol Interact. 2008;176(2-3):121-8. crossref(new window)