Advanced SearchSearch Tips
Effects of Yikgeebohyul-tang Aqueous Extracts on the Rat Hypothyroidism Induced by Propylthiouracil
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Yikgeebohyul-tang Aqueous Extracts on the Rat Hypothyroidism Induced by Propylthiouracil
Son, Ji-Hye; Kim, Dong-Chul;
  PDF(new window)
Objectives The object of this study was to evaluate the effect of Yikgeebohyul-tang aqueous extracts (YKBHT) on the propylthiouracil (PTU)-induced rat hypothyroidism. Methods The rats were divided into 6 groups : intact vehicle control, PTU control, LT4, YKBHT 500, 250 and 125 mg/kg treated groups. Hypothyroidism was induced by daily subcutaneous treatment of PTU 10 mg/kg for 28 days. YKBHT aqueous extracts were administered once a day as an oral dose of 500, 250 and 125 mg/kg for 42 days. The changes were observed : weight of body, thyroid gland, liver, testis, epididymis and prostate, serum thyroid hormone levels, serum male sex hormone levels, serum lipid profiles, liver and testis antioxidant system. These results were compared with LT4 0.5 mg/kg intraperitoneally treated rats. Results These PTU induced hypothyroidism and related hepatic and male reproductive organ damages were favorably and dose-dependently inhibited by treatment of YKBHT 500, 250 and 125 mg/kg, and YKBHT also effectively regulated the PTU-induced abnormal antioxidant defense factor changes in the both liver and testis. Although, LT4 also inhibited PTU-induced hypothyroidism and relative liver damages. But it deteriorated the hypothyroidism related testis, epididymis and prostate damages through testicular oxidative damages. Conclusions : The result of this study suggests that YKBHT has favorable effects on the hypothyroidism and related liver and reproductive organ damages with augmentation of antioxidant defense factor in the testis and liver. YKBHT 500, 250 and 125 mg/kg dose-dependently inhibited PTU-induced hypothyroidism and related liver and male reproductive organ damages in rats.
 Cited by
Joe BY. Clinical thyroidology. 2nd. Seoul:Korea Medicine. 2001:231-55, 409-41.

The Society of Korean Medicine Obstetrics & Gynecology. Korean Medicine Obstetrics & Gynecology Ha. 1st edition. Seoul:Euiseongdang. 2012:234-5, 613.

Ahn SY. Thyroid Clinic. Seoul:Sungbosa. 2004:132-54, 248.

Du HG. Donguishingyehak. Seoul:Institute of Oriental Medicine. 1993:729, 867-74, 1042, 1059-65.

Song YG, Oh YS. tiroidología. Seoul:Korea Medicine. 1995:131-88, 223-47.

Wiersinga WM. Thyroid hormone replacement therapy. Horm Res. 2001;56(1):74-81. crossref(new window)

Kim JJ. Dongyang-uihak Jinryo-yogam(東洋醫學 診療要鑑). Seoul:Institute of Oriental Medicine. 1974:176-7.

Hong JA et al. Effect of Yikgeebohyul-tang on Hair Regrowth and Cytokine Changes on Hair-Removed C57BL/6 Mice. The Journal of Korean Oriental Medicine. 2010;31(1):138-52.

The Society of Daegu Oriental Hospital of Daegu Haany University. Hospital formula. Daegu:Daejin Publish Munhuasa. 1990:143.

Ku SK, Lee YJ. Effects of Lonicerae Flos on the 6-n-propyl-2-thiouracil (PTU)-induced rat hypothyroidism. Korean J. Oriental Physiology & Pathology. 2010;24(4):630-7.

Kim SM, et al. Effects of Bupleuri Radix on the rat hypothyroidism induced by PTU (6-n-propyl-2-thiouracil). Korean J. Oriental Physiology & Pathology. 2012;26(5):714-23.

Mogulkoç R, et al. Influence of hypovolemic and hypertonic treatments on plasma vasopressin levels and fluid balance in the propylthiouraci l-induced hypothyroid rat. Neuro Endocrinol Lett. 1999;20(5):311-4.

O'Connor JC, Frame SR, Ladics GS. Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators. Toxicol Sci. 2002;69(1):79-91. crossref(new window)

Kavutcu M, et al. Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs: effects of vitamins E and C. Nephron. 1996;72(2):269-74. crossref(new window)

Lowry OH, et al. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.

Jamall IS, Smith JC. Effects of cadmium on glutathione peroxidase, superoxidase dismutase and lipid peroxidation in the rat heart: a possible mechanism of cadmium cardiotoxicity. Toxicol Appl Pharmacol. 1985;80(1):33-42. crossref(new window)

Pick E, Keisari Y. Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages-induction by multiple nonphagocytic stimuli. Cell Immunol. 1981;59(2):301-18. crossref(new window)

Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-6. crossref(new window)

Nishikimi M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced PMS and molecular oxygen. Biochem Biophys Res Comm. 1972;46(2):849-54. crossref(new window)

Kakkar P, Das B, Viswanathan PN. Modified spectrophotometric assay of SOD. Indian J Biochem Biophys. 1984;21(2):130-2.

Subudhi U, et al. Supplementation of curcumin and vitamin E enhances oxidative stress, but restores hepatic histoarchitecture in hypothyroid rats. Life Sci. 2009;84(11):372-9. crossref(new window)

Clermont Y. The cycle of the seminiferous epithelium in man. Am J Anat. 1963;112(1):35-51. crossref(new window)

Levene A. Pathological factors influencing excision of tumours in the head and neck. Part I. Clin Otalary. 1981;6(2):145-51. crossref(new window)

Ludbrook J. Update: microcomputer statistics packages. A personal view. Clin Exp Pharmacol Physiol. 1997;24(3):294-6. crossref(new window)

Seoul national University college of Medine. Endocrinology theory. Seoul:Seoul Publish. 1998:189-97.

Kronenberg HM, et al. Williams textbook of endocrinology. 11th ed. Philadelphia:Saunders/Elsevier. 2008:377-409.

Roberts CG, Ladenson PW. Hypothyroidism. Lancet. 2004:363:793-803. crossref(new window)

Lee DW. Dongwon Sipjong-uiseo. Seoul:Daeseoung Munhwasa. 1983:35-7, 86-7.

Heo J. Donguibogam. Seoul:Namsandang. 1983:202-3, 359.

Roti E, et al. The use and misuse of thyroid hormone. Endocr Rev. 1993;14(4):401-24.

Kim MS, et al. Changes in Plasma Leptin Levels Relating to Short-Term Thyroid Manipulation in Rats. Korean Journal of Endocrinology. 2002;17(2):197-205.

Ito M, et al. Effect of levo-thyroxine replacement on non-high-density lipoprotein cholesterol in hypothyroid patients. J Clin Endocrinol Metab. 2007;92(2):608-11. crossref(new window)

Dory L, Roheim PS. Rat plasma lipoproteins and apolipoproteins in experimental hypothyroidism. J Lipid Res. 1981;22(2):287-96.

Williams KV, et al. Fifty years of experience with propylthiouracil-associated hepatotoxicity: what have we learned?. J Clin Endocrinol Metab. 1997;82(6):1727-33.

Choudhury S, Chainy GB, Mishro MM. Experimentally induced hypo- and hyper-thyroidism influence on the antioxidant defence system in adult rat testis. Andrologia. 2003;35(3):131-40. crossref(new window)

Aitken RJ. A free radical theory of male infertility. Reprod Fertil Dev. 1994;6(1):19-24. crossref(new window)

Cooke PS, et al. Increased sperm production in adult rats after transient neonatal hypothyroidism. Endocrinology. 1991;129(1):244-8. crossref(new window)

Sahoo DK, Roy A, Chainy GB. Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chem Biol Interact. 2008;176(2-3):121-8. crossref(new window)