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Gamma-ray-induced skin injury in the mini-pig: Effects of irradiation exposure on cyclooxygenase-2 expression in the skin
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 Title & Authors
Gamma-ray-induced skin injury in the mini-pig: Effects of irradiation exposure on cyclooxygenase-2 expression in the skin
Kim, Joong Sun; Park, Sunhoo; Jang, Won Seok; Lee, Sun Joo; Lee, Seung Sook;
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 Abstract
The basic concepts of radiation-induced skin damage have been established, the biological mechanism has not been studied. In this study, we have examined the effects of gamma rays on skin injury and cyclooxygenase(COX)-2 expression. Gamma irradiation induced clinicopathological changes in a dose- and time-dependent manner in mini-pig skin. The histological changes were consistent with the changes in gross appearance at 12 weeks after irradiation. After three days' irradiation, apoptotic cells in the basal layer were found more frequently in irradiated skin than in normal skin, with the magnitude of the effect being dose-dependent. The thickness of the epidermis transiently increased 3 days after irradiation, and then gradually decreased, although changes in the epithelial thickness of the irradiated field were not observed with irradiation doses over 50 Gy. In the epithelium, there was an initial degenerative phase, during which the rate of basal cell depletion was dependent on the radiation dose (20-70 Gy). One week after irradiation, COX-2 expression was mostly limited to the basal cell layer and was scattered across these cells. High COX-2 expression was detected throughout the full depth of the skin after irradiation. The COX-2 protein is upregulated after irradiation in mini-pig skin. These histological changes associated with radiation exposure dose cause the increased COX-2 expression in a dose-dependent fashion.
 Keywords
Radiation;Pig;Skin;Cyclooxygenase-2;
 Language
Korean
 Cited by
 References
1.
Hopewell JW. The skin: its structure and response to ionizing radiation. Int J Radiat Biol. 1990;57:751-73. crossref(new window)

2.
Lee HJ, Kim SH. Relative biological effectiveness of fast neutrons for apoptosis in mouse hair follicles. J Vet Sci. 2007;8(4):335-40. crossref(new window)

3.
Simon GA, Maibach HI. The pig as an experimental animal model of percutaneous permeation in man: qualitative and quantitative observations-an overview. Skin Pharmacol Appl Skin Physiol. 2000; 13(5):229-34. crossref(new window)

4.
Sullivan TP, Eaglstein WH, Davis SC, Mertz P. The pig as a model for human wound healing. Wound Repair Regen. 2001;9(2):66-76. crossref(new window)

5.
Ma ZF, Chai JK, Yang HM, Liu Q, Xu MH, Yin HN. Acellular porcine dermal matrix produced with different methods and an experimental study on its transplantation to skin wound. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2005;17(2):92-94.

6.
Archambeau JO, Pezner R, Wasserman T. Pathophysiology of irradiated skin and breast. Int J Radiat Oncol Biol Phys. 1995; 31:1171-85. crossref(new window)

7.
Takeuchi K1, Tanaka A, Kato S, Amagase K, Satoh H. Roles of COX inhibition in pathogenesis of NSAID-induced small intestinal damage. Clin Chim Acta. 2010;411(7-8):459-66. crossref(new window)

8.
O'Neill GP, Ford-Hutchinson AW. Expression of mRNA for cyclooxygenase-1 and cyclooxygenase-2 in human tissues. FEBS Lett. 1993;330:156-60.

9.
Athar M, An KP, Morel KD, Kim AL, Aszterbaum M, Longley J, Epstein EH Jr, Bickers DR. Ultraviolet B(UVB)-induced cox-2 expression in murine skin: an immunohistochemical study. Biochem Bioph Res Co. 2001;280(4):1042-7. crossref(new window)

10.
Williams CS, Mann M, DuBois RN. The role of cyclooxygenases in inflammation, cancer, and development. Oncogene. 1999; 18(55):7908-16.

11.
Yeoh AS, Bowen JM, Gibson RJ, Keefe DM. Nuclear factor kappaB (NFkappaB) and cyclooxygenase-2 (Cox-2) expression in the irradiated colorectum is associated with subsequent histopathological changes. Int J Radiat Oncol Biol Phys. 2005;63(5):1295-303. crossref(new window)

12.
Kim JS, Rhim KJ, Jang WS, Lee SJ, Son Y, Lee SS, Park S, Lim SM. $\beta$-Irradiation (166Ho patch)-induced skin injury in the mini pig: effects on $NF-{\kappa}B$ and COX-2 expression in the skin. J Vet Sci. 2015;16(1): 1-9. crossref(new window)

13.
Randall K, Coggle JE. Long-term expression of transforming growth factor TGF beta 1 in mouse skin after localized beta-irradiation. Int J Radiat Biol. 1996;70(3):351-60. crossref(new window)

14.
Morris GM, Hopewell JW. Cell kinetic changes in the follicular epithelium of pig skin after irradiation with single and fractionated doses of X rays. Brit J Radiol 1989;62:41-7. crossref(new window)

15.
Liang L, Hu D, Liu W, Williams JP, Okunieff P, Ding I. Celecoxib reduces skin damage after radiation: selective reduction of chemokine and receptor mRNA expression in irradiated skin but not in irradiated mammary tumor. Am J Clin Oncol-Canc. 2003;26(4):S114-21.