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XPS STUDY ON THE EFFECT OF LOW-ENERGY ELECTRON IRRADIATION ON DNA DAMAGE BY Fe3+ ION
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 Title & Authors
XPS STUDY ON THE EFFECT OF LOW-ENERGY ELECTRON IRRADIATION ON DNA DAMAGE BY Fe3+ ION
Noh, Hyung-Ah; Park, Yeun-Soo; Cho, Hyuck;
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 Abstract
We have employed X-ray photoelectron spectroscopy (XPS) technique to examine the combined effects of low-energy electron (LEE) irradiation and ion on DNA damage. pBR322 plasmid DNA extracted from E. coli ER2420 was used for preparing DNA- sample. The C1s XPS spectra were scanned for LEE-irradiated and LEE-unirradiated samples and then curve-fitted. For the samples with LEE irradiation only or with Fe ion only, no significant changes from pure DNA samples were observed - a single effect of either ion or LEE irradiation did not cause a significant damage. However, when these two components were combined, the DNA damage was increased quite significantly, compared to the sum of DNA damages caused by ion and by LEE irradiation independently. This observation is consistent with our previous results [Radiat. Res. 177, 775 (2012)] which was done using gel-electrophoresis technique. Partial interpretation of the observed spectrum peaks was also attempted.
 Keywords
DNA damage;XPS;Electron beam;Dissociative electron attachment;
 Language
English
 Cited by
 References
1.
Boudaiffa B, Cloutier P, Hunting D, Huels MA, Sanche L. Resonant formation of DNA strand breaks by low-energy(3 to 20 eV) electrons. Science. 2000;287:1658-1660. crossref(new window)

2.
Sanche L. Low energy electron-driven damage in biomolecules. Eur Phys J D. 2005;35:367-390. crossref(new window)

3.
Huels MA, Parenteau L, Sanche L. Reactive scattering of 1-5 eV $O^-$ in films of tetrahydrofuran. J Phys Chem B. 2004;108:16303-16312. crossref(new window)

4.
Barrios R, Skurski P, Simons J. Mechanism for damage to DNA by low-energy electrons. J Phys Chem B. 2002;106:7991-7994. crossref(new window)

5.
Ptasinska S, Sanche L. On the mechanism of anion desorption from DNA induced by low energy electrons. J Chem Phys. 2006;125:144713. crossref(new window)

6.
Berdys J, Anusiewics I, Skurski P, Simons J. Damage to model DNA fragments from very low-energy(<1 eV) electrons. J Am Chem Soc. 2004;126:6441. crossref(new window)

7.
Martin F, Burrow PD, Cai Z, Cloutier P, Hunting D, Sanche L. DNA strand breaks induced by 0-4 eV electrons: The role of shape resonances. Phys Rev Lett. 2004;93:068101. crossref(new window)

8.
Lu QB, Sanche L. Condensed-phase effects ob absolute cross sections for dissociative electron attachment to CFCs and FCFCs adsorbed on Kr. J Chem Phys. 2003;119:2658-2662. crossref(new window)

9.
Lu QB, Sanche L. Enhancement in dissociative electron attachment to $CF_4$, chlorofluorocarbons and hydrofluorocarbons adsorbed on $H_2O$ ice. J Chem Phys. 2004;120:2434-2438. crossref(new window)

10.
Christophorou LG, McCorkle DL, Christodoulides AA. Electron-Molecule Interactions and Their Applications. New York; Academic Press. 1984.

11.
Chutjian A, Garscadden A, Wadehra JM. Electron attachment to molecules at low electron energies. Phys Rep. 1996;264:393-470. crossref(new window)

12.
Ying ZC, Ho WJ. Photodissociation of adsorbed $MO(CO)_6$ induced by direct photoexcitation and hot-electro attachment. 2. Physical mechanisms. J Chem Phys. 1991;94:5701-5714. crossref(new window)

13.
Sanche L. Nanoscopic aspects of electronic aging in dielectrics. IEEE Trans Dielectr Electr Insul. 1997;4:507-543. crossref(new window)

14.
Lu QB, Sanche L. Effects of cosmic rays on atmospheric chlorofluorocarbon dissociation and ozone depletion. Phys Rev Lett. 2001;87:078501. crossref(new window)

15.
Park YS, Cho H, Parenteau L, Bass AD, Sanche L. Cross sections for electron trapping by DNA and its component subunits I: condensed tetrahydrofuran deposited on Kr. J Chem Phys. 2006;125: 074714. crossref(new window)

16.
Voet D, Voet JG, Pratt CW. Fundamentals of Biochemistry. New York; Wiley. 1999.

17.
Park Y, Noh H-A, Cho H. Effect of low-energy electron irradiation on DNA damage by $Fe^{3+}$ ion. Radiation Res. 2012;177;775-780. crossref(new window)

18.
Aruoma OI, Halliwell B, Gajewski E, Dizdaroglu M. Copper-ion-dependent damage to the bases in DNA in the presence of hydrogen-peroxide. Biochem J. 1991;273:601-604. crossref(new window)

19.
Keyer K, Imlay JA. Superoxide accelerates DNA damage by elevating free-iron levels. Proc Natl Acad Sci USA. 1996;93:13635-13640. crossref(new window)

20.
Kasprzak KS. Oxidative DNA and protein damage in metal-induced toxicity and carcinogenesis. Free Radic Biol Med. 2002;32: 958-967. crossref(new window)

21.
Gao YG, Sriram M, Wang AH. Crystallographic studies of metal-ion DNA interactions-different binding modes of cobalt(III), copper(II) and barium(II) to N-7 of guanines in Z-DNA and a drug-DNA complex. Nucleic Acid Res. 1993;21: 4093-4101. crossref(new window)

22.
Lloyd DR, Phillips DH. Oxidative DNA damage mediated by copper(III), iron(II) and nickel(II) Fenton reactions. Mutation Res. 1999;424:23-36. crossref(new window)

23.
Garcia J, Subias G, Cuartero V, Herrero-Martin J. On the correlation between the X-ray absorption chemical shift and the formal valence state in the mixed-valence manganites. J Synchrotron Rad. 2010;17:386-392. crossref(new window)

24.
May CJ, Canavan HE, Castner DG. Quantitative X-ray Photoelectron Spectroscopy and Time-of-Flight Secondary Ion Mass Spectrometry Characterization of the Components in DNA. Anal Chem. 2004;76:1114. crossref(new window)

25.
Peeling J, Hruska FE, McIntyre NS. ESCA spectra and molecular charge distributions for some pyrimidine and purine bases. Can J Chem. 1978; 56:1555. crossref(new window)

26.
Furukawa M, Fujisawa H, Katano S, Ogawawara H, Kim Y, Komeda T, Nilsson A, Kawai M. Geometrical characterization of pyrimidine base molecules adsorbed on Cu(110) surfaces: XPS and NEXAFS studies. Surf Sci. 2003;532-535:261-266. crossref(new window)

27.
Ptasinska S, Stypczynska A, Nixon T, Mason N J, Klyachko D V, Sanche L. X-ray induced damage in DNA monitored by X-ray photoelectron spectroscopy. J Chem Phys. 2008;129:065102. crossref(new window)