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The effect of sterilization condition and UV-C irradiation on the reduction of contamination rate for oyster mushroom bottle culture
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  • Journal title : Journal of Mushroom
  • Volume 13, Issue 3,  2015, pp.256-261
  • Publisher : The Korean Society Of Mushroom Science
  • DOI : 10.14480/JM.2015.13.3.256
 Title & Authors
The effect of sterilization condition and UV-C irradiation on the reduction of contamination rate for oyster mushroom bottle culture
Baek, Il-Sun; Chi, Jeong-Hyun; Jeoung, Yun-Kyeoung; Kim, Jeong-Han; Lim, Jae-Wook;
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 Abstract
This study was conducted to reduce contamination ratio of oyster mushroom bottle cultivation. The optimal conditions of substrate sterilization for reducing of contamination ratio were at for 90 min. In addition, UV-C irradiation is good for lower contamination ratio to continue over 6 hours at cooling and inoculation room after sterilization. The contamination ratio and density of microorganisms of substrate were showed 0% after sterilization at for 90 min. Trichoderma sp., main pathogen of mushrooms, was detected from substrate after sterilized during 2 or 4 hours at and , respectively. The amount of electricity used was the lowest at for 90 min than that of other sterilization conditions. The UV-C irradiation treatment was used UV-C lamp(40 watts) in the inoculation room(). The density of bacteria did not detected after UV-C irradiation for 6 hours. And the death ratio of bacteria and Trichoderma sp. was 99.9% after UV-C irradiation for 6 hours. However, in the same UV-C irradiation time, the death ration of Cladosporium sp. was 90.9%. Therefore, the death ratio of fungi was lower than that of bacteria at the same UV-C irradiation treatment.
 Keywords
Contamination;Sterilization;Trichoderma sp.;UV-C irradiation;
 Language
Korean
 Cited by
 References
1.
Allende A, McEvoy JL, Luo Y, Artes F, Wang CY. 2006. Effectiveness of two-sided UV-C treatments in inhibiting natural microflora and extending the shelf-life of minimally processed 'Red Oak Leaf' lettuce. Food Microbiol. 23:241-249. crossref(new window)

2.
Arts-Hernndez F, Robles PA, Gmez PA, Toms-Callejas A, Arts F. 2010. Low UV-C illumination for keeping overall quality of freshcut watermelon. Postharv Biol Technol. 55:114-120. crossref(new window)

3.
Arts-Hernndez F, Escalona VH, Robles PA, Martnez-Hernndez GB, Arts F. 2009. Effect of UV-C radiation on quality of minimally processed spinach leaves. J Sci Food Agric. 89:414-421. crossref(new window)

4.
Cotxarreara L, Trilla-Gay MI, Steinberg C, Alabouvette C. 2002. Use of sewage sludge compost and Trichoderma asperellum isolates to suppress Fusarium wilt of tomato. Soil Biol and Biochem. 34(4):467-476. crossref(new window)

5.
Cheong JC, Jhune CS, Lee CJ, Oh JA, Shin PG. 2011. Methods of temperature measurement of medium in bottle during sterilization. J Mushroom Sci. 9(2):74-79.

6.
Dennis C, Webster J. 1971a. Antagonistic properties of speciesgroups of Trichoderma. I. Productions of nonvolatile antibiotics. Trans Brit Mycol Soc. 57:25-39. crossref(new window)

7.
Dennis C, Webster J. 1971b. Antagonistic properties of speciesgroups of Trichoderma. II. Productions of nonvolatile antibiotics. Trans Brit Mycol Soc. 57:41-48. crossref(new window)

8.
Elad Y. 2000. Biological control of foliar pathogen by means of Trichoderma harzianum and potential modes of action. Crop Prot. 19(8-10)709-714. crossref(new window)

9.
GARES mushroom research institute. 2008. Handbook of mushroom cultivation. pp168. Gwangju.

10.
Jhune CS, Shin DH, Park JS, Oh SJ. 2000. Effect of pasteurized substrates in various condition on the mycelial growth of oyster mushroom, Pleurotus spp.. Kor J Mycol. 28(2):103-108.

11.
Jo WS, Yun YS, Park SD, Choi BS. 1995. Development of cheap substrate for fruiting of Pleurotus osteatus using paper sludge. Kor J Mycol. 23(3):197-201.

12.
Lee HD, Kim Yg, Choi HG. 2003. Optimum sterilization selection method for increasing yield of Pleurotus ostreatus on the bag-cultivation. Kor J Plant Res. 16(3):218-222.

13.
Obande MA, Tucker GA, Shama G. 2011. Effect of pre-harvest UV-C treatment of tomatoes (Solanum lycopersicon Mill.) on ripening and pathogen resistance. Postharv Biol Technol. 62:188-192. crossref(new window)

14.
Raper KB, Thom C. 1949. A manual of the genus penicillium. The Williams and Wilkins Co., Baltimore. USA.

15.
Schenk M, Raffellini S, Guerrero S, Blanco GA, Alzamora SM. 2011. Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: study of cell injury by flow cytometry. LWT-Food Sci Technol. 44:191-198. crossref(new window)

16.
Tsror L(Lahkim), Barak R, Sneh B. 2001. Biological control of black scurf on potato under organic management. Crop prot. 20(20):145-150. crossref(new window)

17.
Viterbo A, Shoshan H, Dana Friesem, Ofir R, Ilan C. 2001. Antifungal activity of a novel endochitinase gene(chit36) from Trichoderma harzianum Rifai TM. FEMS Microbiol Lett. 200(2):169-174. crossref(new window)