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The effect of sterilization condition and UV-C irradiation on the reduction of contamination rate for oyster mushroom bottle culture
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  • Journal title : Journal of Mushroom
  • Volume 13, Issue 3,  2015, pp.256-261
  • Publisher : The Korean Society Of Mushroom Science
  • DOI : 10.14480/JM.2015.13.3.256
 Title & Authors
The effect of sterilization condition and UV-C irradiation on the reduction of contamination rate for oyster mushroom bottle culture
Baek, Il-Sun; Chi, Jeong-Hyun; Jeoung, Yun-Kyeoung; Kim, Jeong-Han; Lim, Jae-Wook;
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This study was conducted to reduce contamination ratio of oyster mushroom bottle cultivation. The optimal conditions of substrate sterilization for reducing of contamination ratio were at for 90 min. In addition, UV-C irradiation is good for lower contamination ratio to continue over 6 hours at cooling and inoculation room after sterilization. The contamination ratio and density of microorganisms of substrate were showed 0% after sterilization at for 90 min. Trichoderma sp., main pathogen of mushrooms, was detected from substrate after sterilized during 2 or 4 hours at and , respectively. The amount of electricity used was the lowest at for 90 min than that of other sterilization conditions. The UV-C irradiation treatment was used UV-C lamp(40 watts) in the inoculation room(). The density of bacteria did not detected after UV-C irradiation for 6 hours. And the death ratio of bacteria and Trichoderma sp. was 99.9% after UV-C irradiation for 6 hours. However, in the same UV-C irradiation time, the death ration of Cladosporium sp. was 90.9%. Therefore, the death ratio of fungi was lower than that of bacteria at the same UV-C irradiation treatment.
Contamination;Sterilization;Trichoderma sp.;UV-C irradiation;
 Cited by
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