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Effects of Slow Programmable Cryopreservation on Preserving Viability of the Cultured Periodontal Ligament Cells from Human Impacted Third Molar
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  • Journal title : Journal of Korean Dental Science
  • Volume 8, Issue 2,  2015, pp.57-64
  • Publisher : Korean Academy of Dental Sciences
  • DOI : 10.5856/JKDS.2015.8.2.57
 Title & Authors
Effects of Slow Programmable Cryopreservation on Preserving Viability of the Cultured Periodontal Ligament Cells from Human Impacted Third Molar
Kim, Jin-Woo; Kim, Tae-Yi; Kim, Ye-mi; Pang, Eun-Kyoung; Kim, Sun-Jong;
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 Abstract
Purpose: This study was conducted to determine cell viability and differentiation capability of human periodontal ligament (PDL) cells and to elucidate the effects of cryopreservation on the activity of human third molar PDL cells by comparing PDL cells with and without cryopreservation. Materials and Methods: Human PDL fibroblasts obtained from immature third molars were cultured and divided into two groups. The experimental group was cryopreserved with a slow freezing rate of from to followed by plunging in liquid nitrogen at and cultured after fast thawing. The control group was cultured without cryopreservation. Cell viability, growth capacity and morphology were evaluated in both groups. Bivariate statistics were used to compare 2 groups and linear mixed model analysis was used to investigate the growth trends difference over time. Result: Cell viability and growth capacity were not significantly different between the 2 groups (P>0.05). Cultured cell of both groups showed fibroblast-like in appearance, and there were no significant differences in morphology between 2 groups. The mixed model analysis revealed no significant difference of growth capacity between 2 groups over time (${\beta}
 Keywords
Autotransplantation;Cryopreservation;Molar, third;Periodontal ligament;
 Language
English
 Cited by
 References
1.
Andreasen JO, Paulsen HU, Yu Z, Bayer T, Schwartz O. A long-term study of 370 autotransplanted premolars. Part II. Tooth survival and pulp healing subsequent to transplantation. Eur J Orthod. 1990; 12: 14-24. crossref(new window)

2.
Czochrowska EM, Stenvik A, Bjercke B, Zachrisson BU. Outcome of tooth transplantation: survival and success rates 17-41 years posttreatment. Am J Orthod Dentofacial Orthop. 2002; 121: 110-9. crossref(new window)

3.
Oh YH, Che ZM, Hong JC, Lee EJ, Lee SJ, Kim J. Cryopreservation of human teeth for future organization of a tooth bank: a preliminary study. Cryobiology. 2005; 51: 322-9. crossref(new window)

4.
Schwartz O, Andreasen JO, Greve T. Cryopreservation before replantation of mature teeth in monkeys. (II). Effect of preincubation, different freezing and equilibration rates and endodontic treatment upon periodontal healing. Int J Oral Surg. 1985; 14: 350-61. crossref(new window)

5.
Temmerman L, Dermaut LR, De Mil M, Van Maele G, Beele H, De Pauw GA. Influence of cryopreservation on human periodontal ligament cells in vitro. Cell Tissue Bank. 2008; 9: 11-8. crossref(new window)

6.
Paulsen HU, Andreasen JO, Schwartz O. Tooth loss treatment in the anterior region: autotransplantation of premolars and cryopreservation. World J Orthod. 2006; 7: 27-34.

7.
Bartlett PF, Reade PC. Cryopreservation of developing teeth. Cryobiology. 1972; 9: 205-11. crossref(new window)

8.
Schwartz O, Rank CP. Autotransplantation of cryopreserved tooth in connection with orthodontic treatment. Am J Orthod Dentofacial Orthop. 1986; 90: 67-72. crossref(new window)

9.
Temmerman L, De Pauw GA, Beele H, Dermaut LR. Tooth transplantation and cryopreservation: state of the art. Am J Orthod Dentofacial Orthop. 2006; 129: 691-5. crossref(new window)

10.
Kawasaki N, Hamamoto Y, Nakajima T, Irie K, Ozawa H. Periodontal regeneration of transplanted rat molars after cryopreservation. Arch Oral Biol. 2004; 49: 59-69. crossref(new window)

11.
Kamada H, Kaku M, Kawata T, Koseki H, Abedini S, Kojima S, Sumi A, Motokawa M, Fujita T, Ohtani J, Ohwada N, Tanne K. In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field. Am J Orthod Dentofacial Orthop. 2011; 140: 799-805. crossref(new window)

12.
Dissanayake SC, Che ZM, Choi SH, Lee SJ, Kim J. Evaluation of vitrification for cryopreservation of teeth. J Periodontal Implant Sci. 2010; 40: 111-8. crossref(new window)

13.
Schwartz O. Cryopreservation as long-term storage of teeth for transplantation or replantation. Int J Oral Maxillofac Surg. 1986; 15: 30-2. crossref(new window)

14.
Kim Y, Kim MR, Ahn JH, Kim SJ. A simple method for cryopreservation of human periodontal ligament cells in a vitrification solution containing 20% ethylene glycol and 20% dimethyl sulfoxide. Tissue Eng Regen Med Soc. 2011; 8: 415-21.

15.
Kim E, Jeon IS, Kim JW, Kim J, Jung HS, Lee SJ. An MTT-based method for quantification of periodontal ligament cell viability. Oral Dis. 2007; 13: 495-9. crossref(new window)

16.
Andreasen JO, Hckansson L. Atlas of replantation and transplantation of teeth. Philadelphia: WB Saunders; 1992.

17.
Vasconcelos RG, Ribeiro RA, Vasconcelos MG, Lima KC, Barboza CA. In vitro comparative analysis of cryopreservation of undifferentiated mesenchymal cells derived from human periodontal ligament. Cell Tissue Bank. 2012; 13: 461-9. crossref(new window)

18.
Kaku M, Kamada H, Kawata T, Koseki H, Abedini S, Kojima S, Motokawa M, Fujita T, Ohtani J, Tsuka N, Matsuda Y, Sunagawa H, Hernandes RA, Ohwada N, Tanne K. Cryopreservation of periodontal ligament cells with magnetic field for tooth banking. Cryobiology. 2010; 61: 73-8. crossref(new window)

19.
Lee YE, Kim ES, Kim J, Han SH, Lee SJ. The efficacy of programmed cryo-preservation under pressure in rat periodontal ligament cells. J Korean Acad Conserv Dent. 2009; 34: 356-63. crossref(new window)

20.
Mazur P, Leibo SP, Chu EH. A two-factor hypothesis of freezing injury. Evidence from Chinese hamster tissue-culture cells. Exp Cell Res. 1972; 71: 345-55. crossref(new window)

21.
Gao D, Critser JK. Mechanisms of cryoinjury in living cells. ILAR J. 2000; 41: 187-96. crossref(new window)

22.
Kasai M, Mukaida T. Cryopreservation of animal and human embryos by vitrification. Reprod Biomed Online. 2004; 9: 164-70. crossref(new window)

23.
Stevenson DJ, Morgan C, Goldie E, Connel G, Grant MH. Cryopreservation of viable hepatocyte monolayers in cryoprotectant media with high serum content: metabolism of testosterone and kaempherol post-cryopreservation. Cryobiology. 2004; 49: 97-113. crossref(new window)

24.
Ding G, Wang W, Liu Y, An Y, Zhang C, Shi S, Wang S. Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla. J Cell Physiol. 2010; 223: 415-22.

25.
Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, Shi S. Recovery of stem cells from cryopreserved periodontal ligament. J Dent Res. 2005; 84: 907-12. crossref(new window)

26.
Temmerman L, Beele H, Dermaut LR, Van Maele G, De Pauw GA. Influence of cryopreservation on the pulpal tissue of immature third molars in vitro. Cell Tissue Bank. 2010; 11: 281-9. crossref(new window)

27.
Woods EJ, Perry BC, Hockema JJ, Larson L, Zhou D, Goebel WS. Optimized cryopreservation method for human dental pulp-derived stem cells and their tissues of origin for banking and clinical use. Cryobiology. 2009; 59: 150-7. crossref(new window)

28.
Andreasen JO, Paulsen HU, Yu Z, Bayer T. A longterm study of 370 autotransplanted premolars. Part IV. Root development subsequent to transplantation. Eur J Orthod. 1990; 12: 38-50. crossref(new window)

29.
Min HG, Lee J, Shin J, Lee D, Kwak J, Kang N, Kim MR. Autotransplantation of immature third molars; Pulp healing, periodontal healing and root development. J Korean Assoc Oral Maxillofac Surg. 2008; 34: 657-64.