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Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
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  • Journal title : Journal of Korean Dental Science
  • Volume 9, Issue 1,  2016, pp.1-8
  • Publisher : Korean Academy of Dental Sciences
  • DOI : 10.5856/JKDS.2016.9.1.1
 Title & Authors
Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
Jang, Sun Mi; Cho, Sin-Yeon; Kim, Eui-Seong; Jung, Il-Young; Lee, Seung Jong;
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 Abstract
Purpose: The aim of this study was to evaluate the rat periodontal ligament cell viability under MPa condition up to one week using in vivo 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: As soon as 110 upper molar teeth of rats were extracted, they were stored in Hartman`s solution under MPa condition for 1, 2, 3, 4, and 7 days each. All specimens were treated with in vivo MTT assay and the value of optical density was measured by ELISA reader. These values were statistically analyzed by one-way ANOVA. Result: There was no statistical difference on MTT value between immediate and 1 day storage group. There were statistically significant differences between 1 day and 2 days tsorage, 2 and 3 days storage groups, respectively. Teeth of 34, and 7 days storage groups showed significantly lower MTT valuesc ompared with shorter period storage groups. Conclusion: When the MTT values were substituted in standard curve, 1 day storage group at MPa condition showed 68% cell viability when compared with immediate group. It dropped to 13% at 2 days, and to less than 5% at 3 days or more.
 Keywords
MTT assay;Period;Periodontal ligament cell;Tooth storage;
 Language
English
 Cited by
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