Advanced SearchSearch Tips
Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of Korean Dental Science
  • Volume 9, Issue 1,  2016, pp.1-8
  • Publisher : Korean Academy of Dental Sciences
  • DOI : 10.5856/JKDS.2016.9.1.1
 Title & Authors
Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
Jang, Sun Mi; Cho, Sin-Yeon; Kim, Eui-Seong; Jung, Il-Young; Lee, Seung Jong;
  PDF(new window)
Purpose: The aim of this study was to evaluate the rat periodontal ligament cell viability under MPa condition up to one week using in vivo 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: As soon as 110 upper molar teeth of rats were extracted, they were stored in Hartman`s solution under MPa condition for 1, 2, 3, 4, and 7 days each. All specimens were treated with in vivo MTT assay and the value of optical density was measured by ELISA reader. These values were statistically analyzed by one-way ANOVA. Result: There was no statistical difference on MTT value between immediate and 1 day storage group. There were statistically significant differences between 1 day and 2 days tsorage, 2 and 3 days storage groups, respectively. Teeth of 34, and 7 days storage groups showed significantly lower MTT valuesc ompared with shorter period storage groups. Conclusion: When the MTT values were substituted in standard curve, 1 day storage group at MPa condition showed 68% cell viability when compared with immediate group. It dropped to 13% at 2 days, and to less than 5% at 3 days or more.
MTT assay;Period;Periodontal ligament cell;Tooth storage;
 Cited by
Melcher AH. Repair of wounds in the periodontium of the rat. Influence of periodontal ligament on osteogenesis. Arch Oral Biol. 1970; 15: 1183-204. crossref(new window)

Schwartz O, Andreasen JO. Cryopreservation of mature teeth before replantation in monkeys (I). Effect of different cryoprotective agents and freezing devices. Int J Oral Surg. 1983; 12: 425-36. crossref(new window)

Schwartz O, Andreasen JO, Greve T. Cryopreservation before replantation of mature teeth in monkeys. (II). Effect of preincubation, different freezing and equilibration rates and endodontic treatment upon periodontal healing. Int J Oral Surg. 1985; 14: 350-61. crossref(new window)

Hupp JG, Mesaros SV, Aukhil I, Trope M. Periodontal ligament vitality and histologic healing of teeth stored for extended periods before transplantation. Endod Dent Traumatol. 1998; 14: 79-83. crossref(new window)

Soder PO, Otteskog P, Andreasen JO, Modeer T. Effect of drying on viability of periodontal membrane. Scand J Dent Res. 1977; 85: 164-8.

Kim H, Song MJ, Shin SJ, Lee Y, Park JW. Esthetic rehabilitation of single anterior edentulous space using fiber-reinforced composite. Restor Dent Endod. 2014; 39: 220-5. crossref(new window)

Arav A, Natan Y. Directional freezing: a solution to the methodological challenges to preserve large organs. Semin Reprod Med. 2009; 27: 438-42. crossref(new window)

Chesne C, Guillouzo A. Cryopreservation of isolated rat hepatocytes: a critical evaluation of freezing and thawing conditions. Cryobiology. 1988; 25: 323-30. crossref(new window)

Kim E, Jeon IS, Kim JW, Kim J, Jung HS, Lee SJ. An MTT-based method for quantification of periodontal ligament cell viability. Oral Dis. 2007; 13: 495-9. crossref(new window)

Pegg DE. Cryopreservation of vascular endothelial cells as isolated cells and as monolayers. Cryobiology. 2002; 44: 46-53. crossref(new window)

Rubinsky B. Principles of low temperature cell preservation. Heart Fail Rev. 2003; 8: 277-84. crossref(new window)

Lee Y. Evaluation of rat periodontal ligament cell viability after hypothermic preservation under high hydrostatic pressure [PhD dissertation]. Seoul: Graduate School, Yonsei University; 2012.

Hupp JG, Trope M, Mesaros SV, Aukhil I. Tritiated thymidine uptake in periodontal ligament cells of dogs' teeth stored in various media for extended time periods. Endod Dent Traumatol. 1997; 13: 223-7. crossref(new window)

Narayanan AS, Page RC. Connective tissues of the periodontium: a summary of current work. Coll Relat Res. 1983; 3: 33-64. crossref(new window)

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65: 55-63. crossref(new window)

Khedmat S, Dehghan S, Hadjati J, Masoumi F, Nekoofar MH, Dummer PM. In vitro cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay. Restor Dent Endod. 2014; 39: 149-54. crossref(new window)

Henze U, Lennartz A, Hafemann B, Goldmann C, Kirkpatrick CJ, Klosterhalfen B. The influence of the C1-inhibitor BERINERT and the proteinfree haemodialysate ACTIHAEMYL20% on the evolution of the depth of scald burns in a porcine model. Burns. 1997; 23: 473-7. crossref(new window)

Chung JH, Kim J, Choi SH, Kim ES, Park J, Lee SJ. The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure. J Korean Acad Conserv Dent. 2010; 35: 285-94. crossref(new window)

Baek DY, Lee SJ, Jung HS, Kim E. Comparison of viability of oral epithelial cells stored by different freezing methods. J Korean Acad Conserv Dent. 2009; 34: 491-9. crossref(new window)

Du Y, Pribenszky CS, Molnar M, Zhang X, Yang H, Kuwayama M, Pedersen AM, Villemoes K, Bolund L, Vajta G. High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification. Reproduction. 2008; 135: 13-7. crossref(new window)

Pribenszky C, Molnar M, Cseh S, Solti L. Improving post-thaw survival of cryopreserved mouse blastocysts by hydrostatic pressure challenge. Anim Reprod Sci. 2005; 87: 143-50. crossref(new window)

Jeon IS, Kim ES, Kim J, Lee SJ. Evaluation of periodontal ligament cell viability in rat teeth according to various extra-oral dry storage times using MTT assay. J Korean Acad Conserv Dent. 2006; 31: 398-408. crossref(new window)

Kim JW, Kim ES, Kim J, Lee SJ. Evaluation of periodontal ligament cell viability in rat teeth after frozen preservation using in-vivo MTT assay. J Korean Acad Conserv Dent. 2006; 31: 192-202. crossref(new window)

Amemiya H, Matsuzaka K, Kokubu E, Ohta S, Inoue T. Cellular responses of rat periodontal ligament cells under hypoxia and re-oxygenation conditions in vitro. J Periodontal Res. 2008; 43: 322-7. crossref(new window)

Amemiya K, Kaneko Y, Muramatsu T, Shimono M, Inoue T. Pulp cell responses during hypoxia and reoxygenation in vitro. Eur J Oral Sci. 2003; 111: 332-8. crossref(new window)

Smaili SS, Hsu YT, Youle RJ, Russell JT. Mitochondria in Ca2+ signaling and apoptosis. J Bioenerg Biomembr. 2000; 32: 35-46. crossref(new window)