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Antimicrobial activity and cytotoxicity test of Scrophularia ningpoensis hemsl extracts against Klebsiella pneumoniae
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 Title & Authors
Antimicrobial activity and cytotoxicity test of Scrophularia ningpoensis hemsl extracts against Klebsiella pneumoniae
Yook, Keun-Dol;
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 Abstract
Scrophularia ningpoensis hemsl has been traditionally used in China and Vietnam for treatment of bacteria, atopy, pimple, tonsillitis, angina and encephalitis for a long time. The main objectives of this study were to evaluate the antibacterial activity of the Scrophularia ningpoensis hemsl extract on biofilm formation of Klebsiella pneumoniae. Antibacterial activity was conducted using disc diffusion assay and minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined using the broth micro dilution method in accordance to Clinical and Laboratory Standards Institute guidelines(CLSI). Furthermore, cytotoxicity on L929 were assessed using animal cell culture for the proliferation test(MTT cell assay) and the biofilm forming capacity of the K. pneumoniae were determined using the colony forming unit (CFU) assay. The extract exhibited considerable antibacterial activity. K. pneumoniae was susceptible to the extract with the MIC and MBC of 0.1875 and respectively. Cytoxicity test in L929 showed no sign of toxicity at the concentration of and at the same concentration the extract caused inhibition of bacterial biofilm formation. The extract of Scrophularia ningpoensis hemsl possesses an in vitro antibacterial antibiofilm activities against K. pneumoniae, with no sign of cytoxicity on L929.
 Keywords
Anitbacterials activities;Biofilm;Klebsiella pneumoniae;Scrophularia ningpoensis hemsl;
 Language
Korean
 Cited by
 References
1.
Gowsiya Shaik, Sujatha and Santosh Kumar Mehar, "Medicinal plants as source of antibacterial agents to counter Klebsiella pneumoniae", Journal of Applied Pharmaceutical Science Vol. 4(01), pp. 135-147, January, 2014.

2.
Wu HS, Wang FD, Tseng CP, Wu TH, Lin YT, Fung CP, "Characteristics of healthcare associated and community acquired Klebsiella pneumoniae bacteremia in Taiwan", J Infect, Vol. 64, pp. 162-168, 2012. crossref(new window)

3.
Sneh Lata, Geetika Sharma and Harmanjot Kaur Sandhu, "Antibacterial Properties of Various Medicinal Plants Extracts against Klebsiella Sp". International Research Journal of Environment Sciences, Vol. 3(10), pp. 75-78, October 2014.

4.
David L. Paterson, Wen-Chien Ko, Anne Von Gottberg, Sunita Mohapatra, Jose Maria Casellas, "Implications of Extended-Spectrum-Lactamase Production in Nosocomial Infections", Ann Intern Med. Vol. 140 pp. 26-32, 2004. crossref(new window)

5.
Neethu H, Tincy K.T, Jayakumaran A.N, "Comparative Study on the Synergistic Action of Garlic Synthesized and Citrate Capped Silver Nanoparticles with ${\beta}$-Penem Antibiotics" Nanotechnology Vol. 2013, pp. 6, 2013.

6.
Khanuja S.P.S, Arya J.S, Srivastavaetal S.K, "Antibiotic pharmaceutical composition with lysergol as bioenhancer and method of treatment", United States Patent Number 20070060604A1, 2007.

7.
"Pharmacopoeia Commission of the People's Republic of China", Chemical Industry Press: Beijing, China, 2005.

8.
Miyazawa. M, Okuno. Y, Nakamura. S.I, Kameokam. H, "Suppression of SOS-inducing activity of chemical mutagens by cinnamic acid derivatives from Scrophularia ningpoensis in the Salmonella typhymurium TA1535/pSK1002 umu test", J. Agric. Food Chem, Vol. 46, pp. 904-910, 1998. crossref(new window)

9.
Greten. J, Kursbuch Traditionelle Chinesische Medizin, Thieme Verlag, "Iridoids from Scrophularia ningpoensis", Phytochemistry Vol. 28, pp. 2701-2704, 1989. crossref(new window)

10.
Sagare AP, Kuo CL, Chueh FS, Tsay HS, "De novo regeneration of Scrophularia yoshimurae Yamazaki (Scrophulariaceae) and quantitative analysis of harpagoside, an iridoid glucoside, formed in aerial and underground parts of in vitro propagated and wild plants by HPLC", Biol Pharm Bull Vol. 24 pp. 1311-1315, 2001. crossref(new window)

11.
Yen KY, "The Illustrated Chinese Material Medica Crude and Prepared", Taiwan, SMC Publishing Inc Press. pp. 64, 1992.

12.
Liu L, Hudgins WR, Shack S, Yin MQ, Samid D, "Cinnamic acid: a natural product with potential use in cancer intervention", Int J Cancer Vol. 62, pp. 345-350, 1995. crossref(new window)

13.
Garcia D, Fernandez A, Saenz T, Ahumada C, "Antinflammatory effects of different extracts and harpagoside isolated from Scrophularia frutescens L", Farmaco Vol. 51 pp. 443-446, 1996.

14.
De Santos Galindez J, Diaz Lanza A, Fernandez Matellano L, "Biologically active substances from the genus Scrophularia", Pharm Biol Vol. 40, pp. 45-59, 2002. crossref(new window)

15.
Diaz AM, Abad MJ, Fernandez L, Silvan AM, De Santos J, et al. "Phenylpropanoid glycosides from Scrophularia scorodonia", In vitro anti-inflammatory activity. Life Sci Vol. 74, pp. 2515-2526, 2004. crossref(new window)

16.
CLSI, "Performance Standards for Antimicrobial Susceptibility Testing. 26th ed. CLSI supplement M100S", Wayne, PA, Clinical and Laboratory Standards Institute, 2016.