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Purification and Characterization of Mongolian Mare Lactoferrin
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 Title & Authors
Purification and Characterization of Mongolian Mare Lactoferrin
Kim, Kee-Sung; Kim, Ji-Sun; Shin, Mi-Soon; Noh, Hae-Won; Lim, Sang-Dong; Suvd, Duvjir; Alimaa, J.;
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 Abstract
The lactoferrin from mongolian mare colostrum has been purified by gel filtration (Sephadex G-100), affinity chromatography (Toyopear1-AF-Heparin-650M) in two steps. Mare lactoferrin-containing fractions were identified in the first peak among 3 peaks on Sephadex G-100 as first step, and purified lactoferrin was eluted with a step gradient of 0.5M NaCl as a 3 step (gradient 0.1,0.3, 0.5M). Eluted fractions were analyzed by 12% SDS-PAGE, and showed a single protein. Its molecular weight was estimated to be 82kDa. N-terminal amino acid sequence was determined as APRKSVRWCTISPAEXAKXA.
 Keywords
lactoferrin;mare milk;molecular weight;N-terminal sequence;
 Language
English
 Cited by
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2.
Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey, Pakistan Journal of Nutrition, 2015, 14, 8, 517  crossref(new windwow)
 References
1.
Bollage, D. M. and Rozycki, M. D. (1996) Protein methods. 2nd ed. Bollage, D. M. Ed., Wiley-Liss. New York, pp. 107- 154

2.
Brock, J. (1995) Lactoferrin : a multifunctional immunoregulatory protein. Immunol. Today 16, 417-419 crossref(new window)

3.
Hashizume, S., Kuroda, K., and Murakami, M. (1983) Identification of lactoferrin as an essential growth factor for human lymphatic cells lines in serum-free medium. Biochem. Biophys. Acta 763, 377-382 crossref(new window)

4.
Jin, H. S., Keum, J. S., Kim, J. H., and Choi, W. Y. (1995) Purification and physcochemical characterization of lactoferrin from korean native cattles. Korean J. Dairy Sci. 17, 146-160

5.
Jolles, J., Donda, A., Amiguet, P., and Jolles, P. (1984) Mare lactotransferrin : purification, analysis and N-terminal sequence determination. FEBS Lett. 176, 185-188 crossref(new window)

6.
Law, B. A. and Reiter, B. (1977) The isolation and bacteriostatic properties of lactoferrin from bovine milk whey. J. Dairy Res. 44, 595-599 crossref(new window)

7.
Machnicki, M. (1991) Biological properties of lactotransferrin. Folia Biologica 37, 65-76

8.
Nam, M. S. and Yu, D. Y. (1998) Purification of Korean native goat milk lactoferrin by using ion-exchange chromatography and affinity chromatography. Korean J. Dairy Sci. 20, 217-222

9.
Nam, M. S., Nam, K. S., Choe, I. S., Kim, J. W., and Lee, S. W. (1993) Studies on the physiological properties of lactoferrin. Korean J. Dairy Sci. 15, 209-215

10.
Shimazaki, K., Kawano, N., and Yoo, Y. C. (1991) Comparison of bovine, sheep and goat milk lactoferrins in their electrophoretic behavior, confirmation, immunochemical properties and lectin reactivity. Comp. Biochem. Physio. 98B, 417-422 crossref(new window)