JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Evaluation of an Automated ELISA (VIDAS(R)) and Real-time PCR by Comparing with a Conventional Culture Method for the Detection of Salmonella spp. in Steamed Pork and Raw Broccoli Sprouts
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Evaluation of an Automated ELISA (VIDAS(R)) and Real-time PCR by Comparing with a Conventional Culture Method for the Detection of Salmonella spp. in Steamed Pork and Raw Broccoli Sprouts
Hyeon, Ji-Yeon; Hwang, In-Gyun; Kwak, Hyo-Sun; Park, Jong-Seok; Heo, Seok; Choi, In-Soo; Park, Chan-Kyu; Seo, Kun-Ho;
  PDF(new window)
 Abstract
Salmonellosis is an important worldwide foodborne infectious disease that is transmitted by many food vehicles including raw and processed animal products and fresh produce. In this study, the effectiveness of automated ELISA () and realtime PCR in the detection of Salmonella spp. in steamed pork and raw broccoli sprouts was evaluated by comparing their results with those of a conventional culture method. Bulk samples (500 g) of steamed pork and raw broccoli sprouts were inoculated with various levels of Salmonella and divided into 20 samples (25 g each). All the samples, including the controls, were analyzed using a conventional culture method, , and real-time PCR to detect the presence of Salmonella. In addition, the levels of background flora in the steamed pork and the raw broccoli sprouts were determined. In the steamed pork that contained less than 100 CFU/g of aerobic bacteria, all three methods detected low levels of Salmonella without a statistical difference in their performance. In the broccoli sprouts with high quantities of background flora (ca. CFU/g), however, all three methods were unable to detect low levels of Salmonella, and real-time PCR and more sensitively detected Salmonella than the culture method, with significant statistical differences. In conclusion, and real-time PCR could be superior to conventional culture methods in detecting Salmonella in food with high levels of background flora.
 Keywords
Salmonella;culture method;;Real-time PCR;
 Language
Korean
 Cited by
1.
가공식품과 비가공식품에서의 황색포도상구균 검출을 위한 배지법과 Real-time PCR법의 비교,이재훈;송광영;현지연;황인균;곽효선;한정아;정윤희;서건호;

한국축산식품학회지, 2010. vol.30. 3, pp.410-418 crossref(new window)
2.
해산식품과 채소에서 Vibrio parahaemolyticus 검출을 위한 배지배양법과 real-time PCR의 비교검증,천정환;현지연;황인균;곽효선;한정아;정윤희;송광영;서건호;

한국식품과학회지, 2010. vol.42. 3, pp.355-360
3.
Real-Time PCR을 이용한 신선편이 양배추에서 Salmonella spp.의 신속검출,방미경;박승주;김윤지;김지강;오세욱;

한국식품영양과학회지, 2010. vol.39. 10, pp.1522-1527 crossref(new window)
4.
다양한 식품에서 Campylobacter jejuni 검출을 위한 real-time PCR과 배지배양법의 비교검증,천정환;현지연;황인균;곽효선;한정아;김무상;김종현;송광영;서건호;

한국식품과학회지, 2011. vol.43. 1, pp.119-123 crossref(new window)
5.
분말식품에서 Cronobacter spp. 검출을 위한 Real-Time PCR과 배지배양법의 비교검증,천정환;송광영;김선영;현지연;김윤경;황인균;곽효선;서건호;

한국미생물학회지, 2011. vol.47. 1, pp.87-91
6.
즉석섭취편의식품류에서 Staphylococcus aureus 정량검출을 위한 배지검출법과 $TEMPO^{(R)}$ 방법과의 비교검증,송광영;천정환;김혜성;김윤경;김동현;성창현;현지연;박준호;이종익;서건호;

Journal of preventive veterinary medicine, 2011. vol.35. 4, pp.295-298
1.
Comparison of an Automated Most-Probable-Number Technique TEMPO®TVC with Traditional Plating Methods PetrifilmTMfor Estimating Populations of Total Aerobic Bacteria with Livestock Products, Journal of Food Hygiene and Safety, 2012, 27, 1, 103  crossref(new windwow)
2.
Comparison of Standard Culture Method and Real-time PCR Assay for Detection of Staphylococcus aureus in Processed and Unprocessed Foods, Korean Journal for Food Science of Animal Resources, 2010, 30, 3, 410  crossref(new windwow)
3.
Development of blood–yolk–polymyxin B–trimethoprim agar for the enumeration of Bacillus cereus in various foods, International Journal of Food Microbiology, 2013, 165, 2, 144  crossref(new windwow)
4.
Comparison of 3 Selective Media for Enumeration ofBacillus cereusin Several Food Matrixes, Journal of Food Science, 2014, 79, 12, M2480  crossref(new windwow)
5.
Comparison of Real-Time PCR and Culture Methods for Detection of Campylobacter jejuni in Various Foods, Korean Journal of Food Science and Technology, 2011, 43, 1, 119  crossref(new windwow)
6.
Evaluation of a PCR Assay for the Rapid Detection of Staphylococcus aureus in Milk and Meat Products, Korean Journal of Food Science and Technology, 2013, 45, 6, 791  crossref(new windwow)
7.
Comparison of Conventional Culture Method and Real-time PCR for Detection of Yersinia enterocolitica in Sausage and Vegetable Salad, Korean Journal of Food Science and Technology, 2013, 45, 1, 133  crossref(new windwow)
 References
1.
Bansal, N. S., Gray, V., and McDonell, F. (2006) Validated PCR assay for the routine detection of Salmonella in food. J. Food Protect. 69, 282-287

2.
Bohaychuk, V. M., Gensler, G. E., McFall, M. E., King, R. K., and Renter, D. G. (2007) A real-time PCR assay for the detection of Salmonella in a wide variety of food and foodanimal matrices. J. Food Protect. 70, 1080-1087

3.
Catarame, T. M. G., O'Hanlon, K. A., McDowell, D. A., Blair, I. S., and Duffy, G. (2006) Comparison of a real-time polymerase chain reaction assay with a culture method for the detection of Salmonella in retail meat samples. J. Food Safety 26, 1-15 crossref(new window)

4.
Cheung, P. Y., Kwok, K. K., and Kam, K. M. (2007) Application of BAX system, Tecra UniqueTM Salmonella test, and a conventional culture method for the detection of Salmonella in ready-to-eat and raw foods. J. Appl. Microbiol. 103, 219-227 crossref(new window)

5.
D'Aoust, J. Y., Sewell, A. M., and Warburton, D. W. (1992) A comparison of standard cultural methods for the detection of foodborne Salmonella. Int. J. Food Microbiol. 16, 41-50 crossref(new window)

6.
De Medici, D., Pezzotti, G., Marfoglia, C., Caciolo, D., Foschi, G., and Orefice, L. (1998) Comparison between ICSVidas, MSRV and standard cultural method for Salmonella recovery in poultry meat. Int. J. Food Microbiol. 45, 205-210 crossref(new window)

7.
Hein, I., Flekna, G., Krassnig, M., and Wagner, M. (2006) Real-time PCR for the detection of Salmonella spp. in food: An alternative approach to a conventional PCR system suggested by the FOOD-PCR project. J. Microbiol. Methods 66, 538-547 crossref(new window)

8.
Korsak, N., Degeye, J. N., Etienne, G., China, B., and Daube, G. (2004) Comparison of four different methods for Salmonella detection in fecal samples of porcine origin. J. Food Protect. 67, 2158-2164

9.
http://fm.kfda.go.kr/

10.
Krascsenicsova, K., Piknova, L., Kaclikova, E., and Kuchta, T. (2008) Detection of Salmonella enterica in food using two-step enrichment and real-time polymerase chain reaction. Lett. Appl. Microbiol. 46, 483-487 crossref(new window)

11.
Malorny, B., Paccassoni, E., Fach, P., Bunge, C., Martin, A., and Helmuth, R. (2004) Diagnostic real-time PCR for detection of Salmonella in food. Appl. Environ. Microbiol. 70, 7046-7052 crossref(new window)

12.
McMahon, W. A., Schultz, A. M., and Johnson, R. L. (2004) Evaluation of VIDAS Salmonella (SLM) immunoassay method with Rappaport-Vassiliadis (RV) medium for detection of Salmonella in foods: collaborative study. J. AOAC Int. 87, 867-883

13.
Oliveira, S. D., Rodenbusch, C. R., Ce, M. C., Rocha, S. L. and Canal, C. W. (2003) Evaluation of selective and nonselective enrichment PCR procedures for Salmonella detection. Lett. Appl. Microbiol. 36, 217-221 crossref(new window)

14.
Shearer, A. E., Strapp, C. M., and Joerger, R. D. (2001) Evaluation of a polymerase chain reaction-based system for detection of Salmonella Enteritidis, Escherichia coli O157: H7, Listeria spp., and Listeria monocytogenes on fresh fruits and vegetables. J. Food Protect. 64, 788-795

15.
Uyttendaele, M., Vanwildemeersch, K., and Debevere, J. (2003) Evaluation of real-time PCR vs automated ELISA and a conventional culture method using a semi-solid medium for detection of Salmonella. Lett. Appl. Microbiol. 37, 386-391 crossref(new window)

16.
Walker, R. L., Kinde, H., Anderson, R. J., and Brown, A. E. (2001) Comparison of VIDAS enzyme-linked fluorescent immunoassay using Moore swab sampling and conventional culture method for Salmonella detection in bulk tank milk and in-line milk filters in California dairies. Int. J. Food Microbiol. 67, 123-129 crossref(new window)

17.
Wang, X., Jothikumar, N., and Griffiths, M. W. (2004) Enrichment and DNA extraction protocols for the simultaneous detection of Salmonella and Listeria monocytogenes in raw sausage meat with multiplex real-time PCR. J. Food Protect. 67, 189-192

18.
Yeh, K. S., Tsai, C. E., Chen, S. P., and Liao, C. W. (2002) Comparison between VIDAS automatic enzyme-linked fluorescent immunoassay and culture method for Salmonella recovery from pork carcass sponge samples. J. Food Protect. 65, 1656-1659