Advanced SearchSearch Tips
Identification of Hanwoo (Native Korean Cattle Breed) Beef by Real-time PCR Using the MC1R Gene in 5 Provinces of South Korea
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Identification of Hanwoo (Native Korean Cattle Breed) Beef by Real-time PCR Using the MC1R Gene in 5 Provinces of South Korea
Park, Jung-Min; Shin, Jin-Ho; Lee, Dan-Won; Song, Jae-Chul; Suh, Hyung-Joo; Chang, Un-Jae; Kim, Jin-Man;
  PDF(new window)
This paper describes the differentiation between native Korean cattle (Hanwoo) and Holsteins or imported cattle using the real-time polymerase chain reaction (PCR) by targeting the sequence of the melanocortin 1 receptor (MC1R) gene. A rapid and accurate method was developed to identify Hanwoo by genotyping the DNA extracted from 295 commercial beef samples (obtained from 5 provinces in South Korea) labeled as Hanwoo beef. The results of real-time PCR assays for the proportions of Hanwoo were 84, 85.7, 95, 91.4, and 90% in the areas of Seoul, Joongbu, Youngnam, Honam, and Chungcheong, respectively. Thus, the beef samples from 295 butcher shops, which asserted to only sell Hanwoo, showed that 259 of 295 samples were of the Hanwoo beef gene type (T-type) and 36 of 295 samples were Holsteins of imported dairy cattle gene types (C-type or C/T type). In conclusion, the proportion of Hanwoo beef was 87.8% and the proportion of Holstein or imported dairy cattle meat was 12.2% (C-type: 9.8%, C/T-type: 2.4%). Generally, most consumers can not differentiate imported meat from Hanwoo beef. Therefore, Hanwoo beef and imported dairy cattle meat that is sold in butcher shops should have mandatory identification by using MC1R genotyping based on real-time PCR.
Hanwoo (Korean native cattle);real time PCR;MC1R;
 Cited by
Brodomann, P. D. and Moor, D. (2003) Sensitive and semiquantitave TaqManTM real-time polymerase chain reaction systems for the detection of beef (Bos Taurus) and the detection of the family Mammalian in food and feed. Meat Sci. 65, 599-607 crossref(new window)

Cameron, N. D., Van Eijk, M. J., Brugmans, B., and Peleman, J. (2003) Discrimination between selected lines of pigs using AFLP markers. J. Heredity 91, 494-501 crossref(new window)

Crepaldi, P., Fornarelli, F., and Marilli, M. (2005) MC1R gene: comparison between different farm animal species. Ital. J. Anim. Sci. 4, 43-45

Dooley, J. J., Paine, K. E., Garrett, S. D., and Brown, H. M. (2004) Detection of meat species using TaqMan real-time PCR assays. Meat Sci. 68, 431-438 crossref(new window)

Fajardo, V., Gonzalez, I., Lopez-Calleja, I., Martin, I., Hernandez, P. E., Garcia, T., and Martin, R. (2006) PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus). J. Agric. Food Chem. 54, 1144-1150 crossref(new window)

Fajardo, V., Gonzalez, I., Martin, I., Rojas, M., Hernandez, P. E., Garcia, T., and Martin, R. (2008) Differentiation of European wild boar (Sus scrofa scrofa) and domestic swine (Sus scrofa domestica) meats by PCR analysis targeting the mitochondrial D-loop and the nuclear melanocortin receptor 1 (MC1R) genes. Meat Sci. 78, 314-322 crossref(new window)

Fairbrother, K. S., Hopwood, A. J., Lockely, A. K., and Bardsley, R. G. (1998) The actin multigene family and livestock speciation using the polymerase chain reaction. Anim. Biotechnol. 9, 89-100 crossref(new window)

Hird, H., Goodier, R., Schneedem K., Boltz, C., Chisholm, J., Lloyd, J., and Popping, B. (2004) Truncation of oligonucleotide primers confers specificity on real time PCR assays for food authentication. Food Addit Contam . 21, 1035-1040 crossref(new window)

Kim, D. H., Kim, Y. K., Chung, Y. H., Yoo, Y. M., and Park, B. Y. (1993) A study on the consumer's attitude to beef: 1. Consumer's purchasing pattern and preference. RDA. J. Agri. Sci. 35, 598-601

Kim, J. M., Nam, Y. S., Choi, J. H., Lee, M. A., Jeong, J. Y., and Kim, C. J. (2005) Identification of Hanwoo (Korean Native Cattle) Beef in Restaurants using Real-time PCR. Korean J. food Sci. 25, 203-209

Koh, M. C., Lim, C. H., Chua, S. B., Chew, S. T., and Phang, S. T. W. (1998) Random amplified polymerase DNA(RAPD) fingerprints for identification of red meat species. Meat Sci. 48, 275-285 crossref(new window)

Klungland, H., Vage, D. I., Gomez-Raya, L., Adalsteinsson, S., and Lien, E. (1995) The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat colour determination. Mamm. Genome 6, 636-639 crossref(new window)

Krkoska, L., Nebola, M., Steinhauserova, I., Obroska, I., and Ernst, M. (2003) Using the PCR-RFLP method. Fleischwirtschaft Int. 2, 39-42

Livak, K. J. (1999) Allelic discrimination using fluorogenic probes and the 5' nuclease assay. Genet. Anal. 14, 143-149 crossref(new window)

Lockely, A. K. and Bardsley, R. G. (2002) Intron variability in an actin gene can be used to discriminate between chicken and turkey DNA. Meat Sci. 61, 163-168 crossref(new window)

Matsunuga, T., Chiki, K., Tanabe, R., Muroya, S., Shibata, K., Yamada, J., and Shinmura, Y. (1999) A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci. 51, 143-148 crossref(new window)

Pascal, G. and Mahe, S. (2001) Identity, traceability, acceptability and substantial equivalence food. Cell Mol. Biol. 47, 1329-1342

Park, S., D, Kim, T. J., and Lee, J. I. (2005) Identification of Hanwoo and Holstein meat using MGB probe based realtime PCR associated with single nucleotide polymorphism (SNP) in Melanocortin 1 receptor (MC1R) gene. Korean J. Vet. Res. 45, 25-28

Pfeiffer, I., Burger, J., and Brenig, B. (2004) Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle, sheep, goat, roe buck and red deer by PCR-RFLP. Genetics 5, 30-35 crossref(new window)

Rouzaud, F., Martin, J., Gallet, P. F., Delourme, D., Goulemont- Leger, V., Amigues, Y., Menissier, F., Leveziel, H., Julien, R., and Oulmouden, A. (2000) A first genotyping assay of French cattle breeds based on a new allele of the extension gene encoding the melanocortin-1 receptor (Mc1r). Genet. Sel. Evol. 32, 511-520 crossref(new window)

Russo, V., Fontanesi, L., Scotti, E., Tazzoli, M., Dall'Olio, A., and Davoli, R. (2007) Analysis of melanocortin 1 receptor (MC1R) gene polymorphisms in some cattle breeds: Their usefulness and application for breed traceability and authentication of Parmigiano Reggiano cheese. Ital. J. Anim. Sci. 6, 257-272

Searle, A. G. (1968) Comparative genetics of coat colour in mammals. Logos Press, London