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Medium Optimization for Pediocin SA131 Production by Pediococcus pentosaceus SA131 against Bovine Mastitis Using Response Surface Methodology
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 Title & Authors
Medium Optimization for Pediocin SA131 Production by Pediococcus pentosaceus SA131 against Bovine Mastitis Using Response Surface Methodology
Park, Yeo-Lang; Lee, Na-Kyoung; Park, Keun-Kyu; Park, Yong-Ho; Kim, Jong-Man; Nam, Hyang-Mi; Jung, Suk-Chan; Paik, Hyun-Dong;
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Pediococcus pentosaceus SA131 was isolated from jeotgal, is the bacteriocin producer against bovine mastitis pathogens, Streptococcus uberis E290, Enterococcus gallinarum E362, and Staphylococcus epidermidis ATCC 12228. The medium composition for pediocin SA131 production by P. pentosaceus SA131 was optimized using response surface methodology. Component of medium was studied as carbon source (glucose, fructose, lactose, glycerol, sucrose, maltose, and mannitol), nitrogen source (beef extract, yeast extract, peptone, malt extract, and tryptone), mineral and surfactant (, , , , NaCl, sodium acetate, and Tween 80). Through one factor-at-a-time experiment, glucose, fructose, yeast extract, malt extract, NaCl, , and Tween 80 were determined as the good ingredient. The effects of major factors for pediocin SA131 production were investigated by two-level fractional factorial designs (FFD). By a FFD, fructose, yeast extract, and were found to be the important factors for the bacteriocin production. Subsequently, a central composite design (CCD) was adopted to derive a statistical model for optimizing the composition of the fermentation medium. The estimated optimum composition for the production of pediocin SA131 by P. pentosaceus SA131 was as follows; 0.13% fructose, 1% glucose, 1.8% yeast extract, 2.58% , 0.2% NaCl, and 0.2% Tween 80. The pediocin production under optimized medium was increased to 1,000 AU/mL, compared to the 400 AU/mL in MRS medium.
Pediococcus pentosaceus SA131;bacteriocin;pediocin SA131;medium optimization;bovine mastitis;response surface methodology;
 Cited by
백합(Meretrix meretrix)식해에서 분리한 Pediococcus pentosaceus SH-10의 생균제적 특성,송현정;김강진;김희대;유정희;구재근;박권삼;

한국수산과학회지, 2011. vol.44. 6, pp.605-611 crossref(new window)
Bacteriocin Production by a Marine Strain of Bacills sp. Sh10: Isolation, Screening and Optimization of Culture Condition, Biotechnology(Faisalabad), 2014, 13, 6, 273  crossref(new windwow)
Probiotic Properties of Pediococcus pentosaceus SH-10 Isolated from the Hard Clam Meretrix meretrix Shikhae, Korean Journal of Fisheries and Aquatic Sciences, 2011, 44, 6, 605  crossref(new windwow)
Anastasiadou, S., Papagianni, M., Ambrosiadis, I., and Koidis, P. (2008) Rapid quantifiable assessment of nutritional parameters influencing pediocin production by Pediococcus acidilactici NRRL B5627. Bioresource Technol. 99, 6646-6650. crossref(new window)

Anthony, T., Rajesh, T., Kayalvizhi, N., and Gunasekaran, P. (2009) Influence of medium components and fermentation conditions on the production of bacteriocin(s) by Bacillus licheniformis AnBa9. Bioresource Technol. 100, 872-877 crossref(new window)

Barboza-Corona, J. E., Fuente-Salcido, N., Alva-Murillo, N., Ochoa-Zarzosa, A., and Lopez-Meza, J. E. (2009) Activity of bacteriocins synthesized by Bacillus thuringiensis against Staphylococcus aureus isolates associated to bovine mastitis. Vet. Microbiol. 138, 179-183. crossref(new window)

Cheigh, C. I., Choi, H. J., Park, H., Kim, S. B., Kook, M. C., Kim, T. S., Hwang, J. K., and Pyun, Y. R. (2002) Influence of growth conditions on the production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from Kimchi. J. Biotechnol. 95, 225-235. crossref(new window)

Choi, H. J., Cheigh, C. I., Kim, S. B., and Pyun, Y. R. (2000) Production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from Kimchi. J. Appl. Microbiol. 88, 563-571. crossref(new window)

Coelho, M. L. V., Nascimento, J. S., Fagundes, P. C., Madureira, D. J., Oliveira, S. S., Brito, M. A. V. P., and Bastos, M. C. F. (2007) Activity of staphylococcal bacteriocins against Staphylococcus aureus and Streptoccus agalactiae involved in bovine mastitis. Res. Microbiol. 158, 625-630. crossref(new window)

Coenye, T. and Vandamme, P. (2003) Extracting phylogenetic information from whole-genome sequencing projects: the lactic acid bacteria as a test case, Microbiology 149, 3507-3517. crossref(new window)

Delgado, A., Lopez, F. N. A., Brito, D., Peres, C., Fevereiro, P., and Garrido-Fernandez, A. (2007) Optimum bacteriocin production by Lactobacillus plantarum 17.2b requires absence of NaCl and apparently follows a mixed metabolite kinetics. J. Biotechnol. 130, 193-201. crossref(new window)

He, G. Q., Kong, Q., and Ding, L. X. (2004) Response surface methodology for optimizing the fermentation medium of Clostridium butyricum. Lett. Appl. Microbiol. 39, 363-368. crossref(new window)

Kim, H., Eom, H. J., Lee, J. S. Han, J. S., and Han, N. S. (2004) Statistical optimization of medium composition for growth of Leuconostoc citreum. Biotechnol. Bioproc. E. 9, 278-284. crossref(new window)

Kim, M. H., Kong, Y. J., Baek, H., and Hyun, H. H. (2006) Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5. J. Biotechnol. 121, 54-61. crossref(new window)

Lee, N. K., Park, Y. L., Kim, H. W., Park, Y. H., Rhim, S. L., Kim, J. M., Kim, J. M., Nam, H. M., Jung, S. C., and Paik, HD. (2008) Purification and characterization of lacticin NK34 produed by Lactoccous lactis NK34 against bovine mastitis. Korean J. Food Sci. Ani. Resour. 28, 457-462. crossref(new window)

Li, C., Bai, J., Cai, Z., and Ouyang, F. (2002) Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. J. Biotechnol. 93, 27-34. crossref(new window)

Liu, B. L. and Tzeng, Y. M. (1998) Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology. Bioprocess Eng. 18, 413-418.

Miyoshi, A., Rochat, T., Gratadoux, J. J., Le Loir, Y., Oliverira, S. C., Langella, P., and Azevedo, V. (2003) Oxidative stress in Lactococcus lactis. Genet. Mol. Res. 2, 348-359.

Nascimento, J. S., Fagundes, P. C., Brito, M. A. V. P., Santos, K. R. N., and Bastos, M. C. F. (2005) Production of bacteriocins by coagulase-negative staphylococci involved in bovine mastitis. Vet. Microbiol. 106, 61-71. crossref(new window)

Nel, H. A., Bauer, R., Vandamme, E. J., and Dicks, L. M. T. (2001) Growth optimization of Pediococcus darmnosus NCFB1832 and the influence of pH and nutrients on the production of pediocin PD-1. J. Appl. Microbiol. 91, 1131-1138. crossref(new window)

Ochoa-Zarsoza, A., Loeza-Angeles, H., Sahrero-Cisneros, E., Villagomez-Gomez, E. Lara-Zarate, L., and Lopez-Meza, J. E. (2008) Antibacterial activity of thionin Thi2.1 from Arabidopsis thaliana expressed by bovine endothelial cells against Staphylococcus aureus isolates from bovine mastitis. Vet. Microbiol. 127, 425-430. crossref(new window)

Purama, R. K. and Goyal, A. (2008) Screening and optimization of nutritional factors for higher dextransucrase production by Leuconostoc mesenteroides NRRL B-640 using statistical approach. Bioresource Technol. 99, 7108-7114 crossref(new window)

Verellen, T. L. J., Bruggeman, G., Reenen, C. A. V., Dicks, L. M. T., and Vandamme, E. J. (1998) Fermentation optimization of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum 423. J. Ferment. Bioeng. 86, 174-179. crossref(new window)

Wang, Z. W. and Liu, X. L. (2008) Medium optimization for antifungal active substances production from a newly isolated Paenibacillus sp. using surface methodology. Bioresource Technol. 99, 8245-8251. crossref(new window)