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Development of Polyclonal Antibodies to Abdominal and Subcutaneous Adipocyte for Producing Fat-reduced High Quality Pork
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 Title & Authors
Development of Polyclonal Antibodies to Abdominal and Subcutaneous Adipocyte for Producing Fat-reduced High Quality Pork
Choi, Chang-Weon; Baek, Kyung-Hoon; Choi, Chang-Bon; Oh, Young-Kyoon; Hong, Seong-Koo;
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 Abstract
The aim of the present study was to develop polyclonal antibodies to regional inedible adipocytes of pigs and investigate the effect of these antibodies on adipocytes in vitro. As antigens, abdominal and subcutaneous adipocyte PMPs from pigs were injected into sheep 3 times per 3 wk intervals for passive immunization, and non-immunized serum, antisera against abodominal (AAb) or subcutaneous adipocyte PMPs (SAb) were collected before and after the injections. Titers of the antisera obtained from sheep and their cross-reactivities with the heart, kidney, liver, lung, muscle, and spleen of pig were determined by ELISA. Isolation and culture of abdominal and subcutaneous adipocytes from pigs were performed to analyze LDH concentration. At a 1:1,000 dilution, little antibody reactivity was observed for non-immunized serum whereas both AAb and SAb had relatively strong reactivity up to a dilution of 1:16,000. These findings may indicate that strong antibodies against adipocyte PMPs can be developed using an immunological approach. Extremely low reactivity of AAb and SAb was detected with the PMPs of the organs. Both antisera most strongly reacted with each adipocyte PMPs and showed statistically (p<0.05) higher cross-reactivities compared with the non-immunized serum. In conclusion, these results may indicate that the present polyclonal antibodies against regional inedible adipocyte PMPs are well developed and are safe against cross-reactivities with the organs of pigs. Further studies on the in vivo nutritional safety and fat reduction of these antibodies in pigs will be required fat-reduced high quality pork production.
 Keywords
high quality pork;antibody;subcutaneous adipocyte;cross-reactivity;
 Language
Korean
 Cited by
1.
체지방 감소 다클론 후보 항체가 비거세돈의 체중, 분 소화율 및 혈액 대사물질에 미치는 영향,최창원;백경훈;조성백;오영균;홍성구;최창본;

Journal of Animal Science and Technology, 2010. vol.52. 5, pp.375-382 crossref(new window)
2.
흰쥐 체지방 감소를 위한 지방부위별 특이 다클론 항체개발 연구,최창원;박동우;조익환;오영균;장선식;최낙진;감동근;백경훈;

농업생명과학연구, 2012. vol.46. 5, pp.47-55
3.
체지방 감소 다클론 후보 항체가 흰쥐의 체중, in vivo 타장기 안전성 및 혈액성상에 미치는 영향,최창원;

농업생명과학연구, 2013. vol.47. 3, pp.49-59
4.
Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG,;;;;;;

한국축산식품학회지, 2014. vol.34. 3, pp.339-345 crossref(new window)
1.
Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG, Korean Journal for Food Science of Animal Resources, 2014, 34, 3, 339  crossref(new windwow)
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