JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Casein Phosphopeptide (CPP)-Producing Activity and Proteolytic Ability by Some Lactic Acid Bacteria
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Casein Phosphopeptide (CPP)-Producing Activity and Proteolytic Ability by Some Lactic Acid Bacteria
Cho, Yoon-Hee; Oh, Se-Jong;
  PDF(new window)
 Abstract
Casein phosphopeptide (CPP) enhances calcium absorption in humans. Lactic acid bacteria (LAB) are capable of synthesis of cell-surface proteinase, which can hydrolyze milk protein and release several types of peptides in the medium. This study was conducted to characterize proteinase of LAB and to evaluate the CPP production from bovine milk. The content of CPP of milk produced by cell-free extract of LAB was determined based on the quantity of decomposed peptide from casein using the O-phthaldialdehyde (OPA) method. The proteolytic activity of LAB was assayed using fluorescein isothiocyanate (FITC)-labeled casein. Casein appeared to be a better substrate than whey proteins for extracellular proteinases of LAB. During fermentation, milk proteins were hydrolyzed by extracellular proteinase of LAB, resulting in an increase in the amount of free groups. Overall, the results presented here indicate that CPP produced by LAB may be a promising material for novel applications in the dairy industry.
 Keywords
casein phosphopeptide;casein phosphopeptide;lactic acid bacteria;proteolytic activity;
 Language
Korean
 Cited by
1.
재래식 메주에서 분리한 유산균들의 각종 효소활성 및 기능성,정지강;정연비;최혜선;한귀정;박건영;

한국식품영양과학회지, 2010. vol.39. 12, pp.1854-1859 crossref(new window)
2.
전통 발효식품으로부터 Protease 활성을 보유한 유산균의 분리 및 동정,국무창;조석철;박훈;김승섭;변유량;최운용;이현용;

산업식품공학, 2011. vol.15. 2, pp.182-187
3.
Glucose와 Yeast Extract를 이용하여 배양된 유산균을 이용한 하수 일차 슬러지의 가용화,이상민;최한나;신정훈;이은영;

한국미생물생명공학회지, 2012. vol.40. 4, pp.424-429 crossref(new window)
1.
The Anti-inflammatory Effects of Probiotic-produced Exopolysaccharide, Journal of Life Science, 2015, 25, 6, 709  crossref(new windwow)
 References
1.
Adamson, N. J. and Reynolds, E. C. (1995) Characterization of tryptic casein phosphopeptides prepared under industrially relevant conditions. Biotechnol. Bioeng. 45, 196-204. crossref(new window)

2.
Church, F. C., Swaisgood, H. E., Porter, D. H., and Catignani, G. L. (1983) Spectrophotometric assay using O-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J. Dairy. Sci. 66, 1219-1227. crossref(new window)

3.
Corsetti, A., Ombretta, M., Fabio, M., Patrick, F. F., and Marco, G. (2003) Production of caseinophosphopeptides from Na-caseinates prepared from the milk of several species by a proteinase of Lactobacillus helveticus PR4. Food Biotechnol. 17, 183-192. crossref(new window)

4.
Kim, S. H. (1993) Ca nutrition and osteoporosis in Korea. Korean J. Nutr. 26, 203-212.

5.
Kitts, D. D. and Yuan, Y. V. (1992) Caseinophosphopeptides and calcium bioavailability. Trends Food Sci. Technol. 3, 31- 35. crossref(new window)

6.
Klaver, F. A. M., Kingma, F., and Weerkamp, A. H. (1993) Growth and survival of bifidobacteria in milk. Neth. Milk Dairy J. 47, 151-164.

7.
Koroleva, N. S., Lagoda, I. V., Bannikova, L. A., Kosareva, L. V., Popelova, V. V., Rakhimova, N. G., Korelova, D. D., Gudachkova, V. M., and Shkundova, Y. V. (1983) Methods of producing acidophilus cultures. USSR Patent, S.U. 100, 44-74.

8.
Law, J. and Haandrikman, A. (1997) Proteolytic enzymes of lactic acid bacteria. Int. Dairy J. 7, 1-11. crossref(new window)

9.
Lee, J. W., Hwang, Y. S., Hong, S. N., and Im, H. S. (1993) Effects of dietary calcium levels on blood pressure and calcium metabolism in normotensive female young adults with the hypertension family history. Korean J. Nutr. 26, 728-742.

10.
Lee, K. S., Shin, Y. S., Jang, Y. H., Kweon, D. H., Prak, K. M., and Jin, Y. S. (2008) Production of casein phosphopeptides using Streptococcus faecalis var. liquefaciens cell immobilization. Korean J. Biotechnol. Bioeng. 23, 59-64.

11.
Lee, Y. S. and Kim, E. M. (2002) Effect of dietary Ca and Na levels on blood pressure and mineral metabolism in spontaneously hypertensive rats fed high fat diet. Korean J. Nutr. 35, 840-847.

12.
Naito, H. (1986) The mechanism of enhancement in intestinal calcium absorption with phosphopeptides derived during casein digestion. J. Jpn. Soc. Nutr. Food Sci. 39, 433-439. crossref(new window)

13.
Park, K. S. and Lee, K. A. (2002) A case study on the effect of Ca intake on depression and anxiety. Korean J. Nutr. 35, 45-52.

14.
Schaafsma, G. (1997) Bioavailability of calcium. IDF Bulletin. 322, 20-24.

15.
Shihata, A. and Shah, N. P. (2000) Proteolytic profiles of yogurt and probiotic bacteria. Int. Dairy J. 10, 401-408. crossref(new window)

16.
Singh, J. and Sharma, D. K. (1983) Proteolytic breakdown of casein and its fraction by lactic acid bacteria. Milchwissenschaft 238, 148-149.

17.
Twining, S. S. (1984) Fluorescin Isothiocyanate-labeled casein assay for proteolytic enzymes. Anal. Biochem. 143, 30-34. crossref(new window)