Advanced SearchSearch Tips
The Effects of Dietary Soybean Fermented with Aspergillus oryzae or Bacillus natto on Egg Production and Egg Lipid Composition in Layer
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
The Effects of Dietary Soybean Fermented with Aspergillus oryzae or Bacillus natto on Egg Production and Egg Lipid Composition in Layer
Hong, Hee-Ok; Abanto, Oliver D.; Kim, Ki-Hyun; Nam, Ki-Taeg; Son, Jong-Youn; Jung, Woo-Suk; Nam, In-Sik; Hwang, Seong-Gu;
  PDF(new window)
This study was conducted to determine the effects of dietary low grade soybean, fermented with Aspergillus oryzae (FSB 1) or Bacillus subtilis var. natto (FSB 2), on egg production and quality, fat and cholesterol content, and the fatty acid (FA) profile of eggs by lipid layer. A total of 18 Hi-Line strain layers, 22 wk of age, were randomly assigned to three dietary treatments: no fermented soybean (control), control with 15% FSB 1 (C + FSB 1), and control with 15% FSB 2 (C + FSB 2). The rate of egg production and egg weight were evaluated between two periods: one was from the 1st to 4th wk and the other was from the 5th to 8th wk. At the 8th wk, a total of 30 eggs were randomly selected from each treatment group and analyzed for physical quality, fat content, fatty acid composition and cholesterol content. The results showed that egg production was increased in hens fed with diets containing fermented soybeans from the 5th to 8th wk period (p<0.01). A similar tendency was observed through eight weeks` cumulative egg production (p<0.05). There were no significant differences in egg production between the C + FSB 1 and C + FSB 2 treatment groups (p>0.05). Egg weight and other physical properties did not vary between treatment groups (p>0.05). Egg yolks among different treatment groups were similar in fat content, but egg yolks in the C + FSB 1 and C + FSB 2 groups had lower oleic acid (p<0.05), higher linoleic, -linolenic, and arachidonic acids (p<0.01), and lower cholesterol content (p<0.05) than those in the control group. In conclusion, supplementation of fermented low grade soybeans might be useful as a functional feedstuff to improve egg production and quality for a healthy human diet.
fermented soybean;egg production;fatty acid composition;egg cholesterol;laying hen;
 Cited by
Abdulrahim, S. M., Haddadin, S. Y., Hashlamoun, E. A., and Robinson, R. K. (1996) The influence of Lactobacillus acidophilus and bacitracin on layer performance of chicken and cholesterol content of plasma and egg yolk. Br. Poult. Sci. 37, 341-346. crossref(new window)

Anthony, M., Clarkson, T., Weddle, D., and Wolfe. M. (1995) Effect of soy protein and phytoestrogens on cardiovascular risk factors in rhesus monkey. J. Nutr. 125, 803S-804S.

AOAC (1990) Official Methods of Analysis. 15th ed., Association of Official Analytical Chemists, Washington, DC.

Boue, S. M., Carter, C. H., Ehrlich, K. C., and Cleveland, T. E. (2000) Induction of the soybean phytoalexins coumestrol and glyceollin by Aspergillus. J. Agric. Food Chem. 48, 2167-2172. crossref(new window)

Carroll, K. (1991) Review of clinical studies on cholesterollowering response to soy protein. J. Am. Diet Assoc. 91, 820-827.

Chah, C. C., Nelson., R. A. Carlson, C. W., Semeniuk, G., Palmer, I. S., and Hesseltine, C. W. (1976) Fungus-fermented soybeans benefit the life cycle of Japanese quail (Coturnix coturnix japonica). Poult. Sci. 55, 975-981. crossref(new window)

Chowdhury, S. R., Chowdhurry, S. D., and Smith, T. K. (2002) Effects of dietary garlic on cholesterol metabolism in laying hens. Poult. Sci. 81, 1856-1862. crossref(new window)

Chung, W.-Y., Kim, S.-K., and Son, J.-Y. (2008) Isoflavones contents and physiological activities of soybeans fermented with Aspergillus oryzae or Bacillus natto. J. Korean Soc. Food Sci. Nutr. 37, 141-147. crossref(new window)

Diel, P., Smolnikar, K., Schultz, T., Laudenbach-Leschowsky, U., Michna, H., Bolt, H. M., and Vollmer, G. (2001) Phytoestrogens and carcinogenesis - Differential effects of genistein in experimental models of normal and malignant rat endometrium. Hum. Reprod. 16, 997-1006. crossref(new window)

Fasina, Y. O., Garlich, J. D., Classen, H. L., Ferket, P. R., Havenstein, G. B., Grimes, J. L., Qureshi, M. A., and Christensen, V. L. (2004) Response of turkey poults to soybean lectin levels typically encountered in commercial diets. 1. Effect on growth and nutrient digestibility. Poult. Sci. 83, 1559-1571. crossref(new window)

Feng, J., Liu, X., Xu, Z. R., Liu, Y. Y., and Lu, Y. P. (2007a) Effects of Aspergillus oryzae 3.042 fermented soybean meal on growth performance and plasma biochemical parameters in broilers. Anim. Feed Sci. Technol. 134, 235-242. crossref(new window)

Feng, J., Liu, X., Xu, Z. R., Lu, Y. P. and Liu, Y. Y. (2007b) Effects of Aspergillus oryzae fermented soybean meal on growth performance, digestibility of dietary components and activities of intestinal enzymes in weaned piglets. Anim. Feed Sci. Technol. 134, 295-303. crossref(new window)

Feng, J., Liu, X., Xu, Z. R., Wang, Y. Z., and Liu, J. X. (2007c) Effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers. Poult. Sci. 86, 1149-1154. crossref(new window)

Ford, J. A. Jr., Clark, S. G., Walters, E. M., Wheeler, M. B., and Hurley, W. L. (2006) Estrogenic effects of genistein on reproductive tissues of ovareictomized gilts. J. Anim. Sci. 84, 834-842.

Hirabayashi, M., Matsui, T., Yano, H., and Nakajima, T. (1998) Fermentation of soybean meal with Aspergillus usamii reduces phosphorus excretion in chicks. Poult Sci. 77, 552-556. crossref(new window)

Hong, K. J., Lee, C. H., and Kim, S. W. (2004) Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals. J. Med. Food. 7, 430-435. crossref(new window)

Jackson, C.-J. C., Dini, J. P., Lavandier, C., Rupasinghe, H. P. V., Faulkner, H., Poysa, V., Buzzell, D. and DeGrandis, S. (2002) Effects of processing on the content and composition of isoflavones during manufacturing of soy beverage and tofu. Process Biochem. 37, 1117-1123. crossref(new window)

Jeff-Agboola, Y. A. and Oguntuase, O. S. (2006) Effects of Bacillus sphaericus on proximate composition of soybean (Glycine max) for the production of Soy Iru. Pakistan J. Nutri. 5, 606-607. crossref(new window)

Jiang, R., Chang, X., Stoll, B., Ellis, K. J., Shypallo, R. J., Weaver, E., Campbell, J., and Burrin, D. G. (2000) Dietary plasma proteins used more efficiently than extruded soy protein for lean tissue growth in early-weaned pigs. J. Nutr. 130, 2016-2019.

Jiang, Y. Z., Jiang, S. Q., Lin, Y. C., Xi, P. B., Yu, D. Q. and Wu, T. X. (2007) Effects of soybean isoflavone on growth performance, meat quality, and antioxidation in male broilers. Poult. Sci. 86, 1356-1362. crossref(new window)

Kim, J. H., Hwangbo, J., Choi, N.-J., Park, H. G., Yoon, D.-H., Park, E.-W., Lee, S.-H., Park, B.-K., and Kim, Y. J. (2007) Effect of dietary supplementation with conjugated linoleic acid, with oleic, linoleic, or linolenic acid, on egg quality characteristics and fat accumulation in the egg yolk. Poult. Sci. 86, 1180-1186. crossref(new window)

Li, D. F., Nelssen, J. L., Reddy, P. G., Blecha, F., J. Hancok, D., Allee, G., Goodb, R. D., and Klemm, R. D. (1990) Transient hypersensitivity to soybean meal in the early weaned pig. J. Anim. Sci. 68, 1790-1799.

Liu, J., Chang, S. K. C., and Wiesenborn, D. (2005) Antioxidant properties of soybean isoflavone extract and tofu in vitro and in vivo. J. Agric. Food Chem. 53, 2333-2340. crossref(new window)

Mathivanan, R., Selvaraj, P., and Nanjappan, K. (2006) Feeding of fermented soybean meal on broiler performance. Int. J. Poult. Sci. 5, 868-872. crossref(new window)

Micevych, P. and Sinchak, K. (2008) Estradiol regulation of progesterone synthesis in the brain. Mol. Cell Endocrinol. 290, 44-50. crossref(new window)

Mimura, A. and Yasaki, S. (1998) Microbial transformation of soybean germ components to antioxidative isoflavonoids. Soy Protein Res. 1, 46-51.

NAS-NRC (National Academy of Science, National Research Council). (1984) Nutrient Requirements of Poultry. Washington, DC.

PABTFF-NRC (Panel on the Application of Biotechnology to Traditional Fermented Foods, National Research Council). (1992) Application of Biotechnology in Traditional Fermented Foods. The National Academies Press, Washington, DC.

Potter, S. (1998) Soy protein and cardiovascular disease: the impact of bioactive components in soy. Nutr. Rev. 56, 231-235.

Rios, D. R. A., Rodrigues, E. T., Cardoso, A. P. Z., Montes, M. B. A., Franceschini, S. A., and Toloi, M R. T. (2008) Lack of effects of isoflavones on the lipid profile of Brazilian postmenopausal women. Nutr. 24, 1153-1158. crossref(new window)

Ruiz-Larrea, M. B., Mohan, A. R., Paganga, G., Miller, N. J., Bolwell, G. P., and Riceevans, C. A. (1997) Antioxidant activity of phytoestrogenic isoflavones. Free Radic. Res. 26, 63-70. crossref(new window)

Sahin, N., Onderci, M., Balci, T. A., Cikim, G., Sahin, K., and Kucuk, O. (2007) The effect of soy isoflavones on egg quality and bone mineralization during the late laying period of quail. Br. Poult. Sci. 48, 363-369. crossref(new window)

Salma, U., Miah, A. G., Tareq, K. M. A., Maki, T., and Tsujii, H. (2007) Effect of dietary Rhodobacter capsulatus on eggyolk cholesterol and laying hen performance. Poult. Sci. 86, 714-719. crossref(new window)

Thorp, A. A., Howe, P. R., Mori, T. A., Coates, A. M., Buckley, J. D., Hodgson, J., Mansour, J., and Mever, B. J. (2008) Soy food composition does not lower LDL cholesterol in either equol or nonequol producers. Am. J. Clin. Nutr. 88, 298-304.

Wei, Q.-K., Chen, T.-R., and Chen, J.-T. (2008) Use of Bacillus subtilis to enrich isoflavone aglycones in fermented natto. J. Sci. Food Agric. 88, 1007-1011. crossref(new window)

Wei, H., Rowen, R., Cai, Q., Barnes, S., and Wang, Y. (1995) Antioxidant and antiproliferative effect of soybean isoflavon genistein. Proc. Soc. Exp. Biol. Med. 208, 124-130. crossref(new window)

Woclawek-Potocka, I., Bah, M. M., Korzekwa, A., Pistula, M. K., Wiczkowski, W., Depta, A., and Skarzynski, D. J. (2005) Soybean-derived phytoestrogens regulate prostaglandin secretion in endometrium during cattle estrous cycle and early pregnancy. Exp. Biol. Med. 230, 189-199.

Wood, B. J. B. (1998) Microbiology of Fermented Foods. Vol. 2. 2nd ed. Springer, USA.

Xu, C.-L., Ji, C., Ma, Q., Hao, K., Jin, Z.-Y., and Li, K. (2006) Effects of a dried Bacillus subtilis culture on egg quality. Poult. Sci. 85, 364-368. crossref(new window)

Yousef, M. I., Kamel, K. I., Esmail, A. M., and Baghdadi, H. H. (2004) Antioxidant activities and lipid lowering effects of isoflavon in male rabbits. Food Chem. Toxicol. 42, 1497-1503. crossref(new window)

Zamora, R. G. and Veum, T. L. (1988) Nutritive value of whole soybean fermented with Asperdillus oryzae and Rhizopus oligosporus as evaluated by neonatal pigs. J. Nutr. 118, 438-444.

Zamora, R. G. and Veum, T. L. (1979) The nutritive value of dehulled soybeans fermented with Aspergillus oryzae or Rhizopus oligosporus as evaluated by rats. J. Nutr. 109, 1333-1339.