JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Plant Oils and Minerals for the Inhibition of Lipase Activity of Staphylococcus aureus Isolated from Fermented Pork Meat
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Plant Oils and Minerals for the Inhibition of Lipase Activity of Staphylococcus aureus Isolated from Fermented Pork Meat
Cho, Sang-Buem; Chang, Woo-Kyung; Kim, Yun-Jung; Moon, Hyung-In; Joo, Jong-Won; Choi, In-Soo; Seo, Kun-Ho; Kim, Soo-Ki;
  PDF(new window)
 Abstract
Staphylococcus aureus lipase is regarded as a virulence factor. The response of lipase activity to various factors can provide important insights concerning the prevention of S. aureus during meat fermentation. This study was conducted to evaluate the main effects of nutrients used in culture media, and their combined effects on the inhibition of lipase activity and cell growth of pathogenic S. aureus SK1593 isolated from fermented pork meat. A Plackett-Burman design was used to evaluate the main effects of variables, including olive oil, soybean oil, grapeseed oil, sesame oil, , , , , and KCl. Significant negative effects on lipase activity were detected with soybean oil, grapeseed oil, , and . Additionally, these nutrients were further selected as variables for the investigation of their combined effect on lipase activity, via response surface methodology. In order to confirm the regression model, a situation that only inhibits lipase activity was simulated. The predicted lipase activity and cell growth of the simulated situation were 14.0 U/mL and (CFU/mL), respectively, and the estimated value of those in the same medium showed 15.14 U/mL and (CFU/mL) respectively. The lipase activity of the simulated medium was inhibited approximately 5-fold as compared to the basal medium, but no significant differences in cell counts were noted to exist between the basal and simulated media. These results suggest that soybean oil, grapeseed oil, , and can be used to inhibit the growth of pathogenic S. aureus during the process of meat fermentation.
 Keywords
Staphylococcus aureus SK1953;lipase activity;Plackett-Burman design;response surface model;
 Language
English
 Cited by
1.
볏짚 유래 수용성 탄수화물 생산에 있어 cellulase, hemicellulase 및 xylanase 최적혼합조건,조상범;이상석;김창현;류경선;박희준;명현;최낙진;

생명과학회지, 2012. vol.22. 1, pp.74-79 crossref(new window)
2.
Optimization of Carbon Sources to Improve Antioxidant Activity in Solid State Fermentation of Persimmon peel Using Lactic Acid Bacteria,;;;

한국초지조사료학회지, 2012. vol.32. 4, pp.361-368 crossref(new window)
3.
양돈용 사료 첨가제 개발을 위하여 구기자 부산물로부터 메탄올수용액을 이용한 총 폴리페놀 추출조건 최적화,심관섭;나종삼;오성진;최낙진;

한국유기농업학회지, 2012. vol.20. 1, pp.91-99
4.
감초폴리페놀 추출효율에 있어 열처리, 에탄올 농도, 추출시간 및 용매비율이 미치는 영향 탐색,채정일;류경선;서강석;김경훈;오영균;장선식;최창원;최낙진;

한국유기농업학회지, 2012. vol.20. 3, pp.399-409
1.
Optimization of Carbon Sources to Improve Antioxidant Activity in Solid State Fermentation of Persimmon peel Using Lactic Acid Bacteria, Journal of The Korean Society of Grassland and Forage Science, 2012, 32, 4, 361  crossref(new windwow)
2.
Optimum Enzyme Mixture of Cellulase, Hemicellulase, and Xylanase for Production of Water-Soluble Carbohydrates from Rice Straw, Journal of Life Science, 2012, 22, 1, 74  crossref(new windwow)
 References
1.
Al-Waili, N. S. (2005) Mixture of honey, beeswax and olive oil inhibits growth of Staphylococcus aureus and Candida albicans. Arch. Med. Res. 36, 10-13. crossref(new window)

2.
Ananou, S., Garriga, M., Jofre, A., Aymerich, T., Gavez, A., Maqueda, M., Martiez-Bueno, M., and Valdivia, E. (2010) Combined effect of enterocin AS-48 and high hydrostatic pressure to control food-borne pathogens inoculated in low acid fermented sausages. Meat Sci. 84, 594-600. crossref(new window)

3.
Ananou, S., Maqueda, M., Martiez-Bueno, M., Gavez, A., and Valdivia, E. (2005) Control of Staphylococcus aureus in sausages by enterocin AS-48. Meat Sci. 71, 549-556. crossref(new window)

4.
Box, G. E. P., and Behnken, D. W. (1960) Some new three level designs for the study of quantitative variables. Technometrics. 2, 455-475. crossref(new window)

5.
Chauhan, K., Trivedi, U., and Patel, K. C. (2007) Statistical screening of medium components by Plackett-Burman design for lactic acid production by Lactobacillus sp. KCP01 using date juice. Bioresour. Technol. 98, 98-103. crossref(new window)

6.
Ebrahimpour, A., Rahman, R., Ean Ch'ng, D., Basri, M., and Salleh, A. (2008) A modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM. BMC Biotech. 8, 96-101. crossref(new window)

7.
Gonzaez-Fandos, M. E., Sierra, M., Garci-Lopez, M. L., Garci-Fernadez, M. C., and Otero, A. (1999) The influence of manufacturing and drying conditions on the survival and toxinogenesis of Staphylococcus aureus in two Spanish dry sausages (chorizo and salchichon). Meat Sci. 52, 411-419. crossref(new window)

8.
Grippa, E., Valla, R., Battinelli, L., Mazzanti, G., Saso, L., and Silvestrini, B. (1999) Inhibition of Candida rugosa lipase by berberine and structurally related alkaloids, evaluated by high-performance liquid chromatography. Biosci. Biotechnol. Biochem. 63, 1557-1562. crossref(new window)

9.
Gupta, R., Gupta, N., and Rathi, P. (2004) Bacterial lipases: an overview of production, purification and biochemical properties. Appl. Microbiol. Biotechnol. 64, 763-781. crossref(new window)

10.
Hasanuzzaman, M., Umadhay-Briones, K. M., Zsiros, S. M., Morita, N., Nodasaka, Y., Yumoto, I., and Okuyama, H. (2004) Isolation, identification, and characterization of a novel, oil-degrading bacterium, Pseudomonas aeruginosa T1. Curr. Microbiol. 49, 108-114.

11.
Jofre, A., Aymerich, T., and Garriga, M. (2008) Assessment of the effectiveness of antimicrobial packaging combined with high pressure to control Salmonella sp. in cooked ham. Food Control. 19, 634-638. crossref(new window)

12.
Joseph, B., Ramteke, P. W., and Kumar, P. A. (2006) Studies on the enhanced production of extracellular lipase by Staphylococcus epidermidis. J. Gen. Appl. Microbiol. 52, 315-320. crossref(new window)

13.
Kuroda, M., Nagasaki, S., Ito, R., and Ohta, T. (2007) Sesquiterpene farnesol as a competitive inhibitor of lipase activity of Staphylococcus aureus. FEMS Microbiol. Lett. 273, 28-34. crossref(new window)

14.
Lakshmi, B. S., Kangueane, P., Abraham, B., and Pennathur, G. (1999) Effect of vegetable oils in the secretion of lipase from Candida rugosa (DSM 2031). Lett. Appl. Microbiol. 29, 66-70. crossref(new window)

15.
Lappe, R., Motta, A. S., Sant'Anna, V., and Brandelli, A. (2009) Inhibition of Salmonella enteritidis by cerein 8A, EDTA and sodium lactate. Int. J. Food Microbiol. 135, 312- 316. crossref(new window)

16.
Longshaw, C. M., Farrell, A. M., Wright, J. D., and Holland, K. T. (2000) Identification of a second lipase gene, gehD, in Staphylococcus epidermidis: comparison of sequence with those of other staphylococcal lipases. Microbiology. 146, 1419-1427.

17.
Pereira, V., Lopes, C., Castro, A., Silva, J., Gibbs, P., and Teixeira, P. (2009) Characterization for enterotoxin production, virulence factors, and antibiotic susceptibility of Staphylococcus aureus isolates from various foods in Portugal. Food Microbiol. 26, 278-282. crossref(new window)

18.
Plackett, R. L., and Burman, J. P. (1946) The design of optimum multifactorial experiments. Biometrika. 33, 305-325. crossref(new window)

19.
Rathi, P., Goswami, V. K., Sahai, V., and Gupta, R. (2002) Statistical medium optimization and production of a hyperthermostable lipase from Burkholderia cepacia in a bioreactor. J. Appl. Microbiol. 93, 930-936. crossref(new window)

20.
Rosenstein, R., and Gotz, F. (2000) Staphylococcal lipases: Biochemical and molecular characterization. Biochimie. 82, 1005-1014. crossref(new window)

21.
Ryu, H., Kim, H., Choi, W., Kim, M., Park, S., Han, N., Oh, T., and Lee, J. (2006) New cold-adapted lipase from Photobacterium lipolyticum sp. nov. that is closely related to filamentous fungal lipases. Appl. Microbiol. Biotechnol. 70, 321-326. crossref(new window)

22.
Tamura, K., Dudley, J., Nei, M., and Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596-1599. crossref(new window)

23.
Tamura, K., Nei, M., and Kumar, S. (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. U S A. 101, 11030-11035. crossref(new window)

24.
Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680. crossref(new window)

25.
Walavalkar, G. S., and Bapat, M. M. (2002) Staphylococcus warneri BW 94--a new source of lipase. Indian J. Exp. Biol. 40, 1280-1284.