JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Inhibitory Effect of Polysaccharide from Kefir Grain on the Infection of MA-104 Cell by Human Rotavirus
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Inhibitory Effect of Polysaccharide from Kefir Grain on the Infection of MA-104 Cell by Human Rotavirus
Lee, Jong-Ik; Song, Kwang-Young; Chon, Jung-Whan; Hyeon, Ji-Yeon; Seo, Kun-Ho;
  PDF(new window)
 Abstract
The inhibitory effect of a polysaccharide from kefir on human rotavirus infection in MA-104 cells was investigated. The extracted polysaccharide was separated as fraction I in unbound materials and as fractions II, III, and IV in bound materials. Adding polysaccharide fractions II (4.8 mg/mL), III (5.3 mg/mL), and IV (1.4 mg/mL) inhibited the infection of MA-104 cells by human rotavirus. The 50% inhibitory concentrations (IC 50) were 0.075, 0.083, and 0.022 mg/mL, respectively. Based on these results, the kefir polysaccharide has anti-rotavirus activity. In conclusion, a polysaccharide from kefir had more than a 97% inhibition effect against human rotavirus infection in MA-104 cells.
 Keywords
kefir;polysaccharide;human rotavirus;
 Language
Korean
 Cited by
 References
1.
Dasilva, J. A. L. and Goncalves, M. P. (1990) Studies on a purification method for locust bean gum by precipitation with isopropanol. Food Hydrocolloid 4, 277-287. crossref(new window)

2.
Ferreira, I. M., Pinho, O., Monteiro, D., Faria, S., Cruz, S., Perreira, A., Roque, A.C., and Tavares, P. (2010) Effect of kefir grains on proteolysis of major milk proteins. J. Dairy Sci. 93, 27-31. crossref(new window)

3.
Hosono, A., Tanabe, T., and Otani, H. (1990) Binding properties of lactic acid bacteria isolated from kefir milk with mutagenic amino acid pyrolyzates. Milchwissenschaft 45, 647-651.

4.
Ismail, A. A., EL-Nockrashy, S. A., and Khorshid, M. A. (1983) A beverage from separated bufallo milk fermented with kefir grains. J. Dairy Technol. 36, 117-118. crossref(new window)

5.
Kaljot, K. T., Shaw, R. D., Rubin, D. H., and Greenberg, B. H. (1988) Infectious rotavirus enters cells direct cell membrane penetration, not by endocytosis. J. Virol. 62, 1136-1144.

6.
Kandler, O. and Kunath, P. (1983) Lactobacillus kefir a component of the microflora of kefir. Syst. Appl. Microbiol. 4, 286-294. crossref(new window)

7.
Kemp, N. (1984) Kefir, the champagne of cultured dairy products. Cultured Dairy Prod. J. 19, 29-30.

8.
Kim, J. H. (2003) Studies in the characteristics of roby lactic acid bacteria and exopolysaccharide isolated from kefir culture. M.S. thesis, Konkuk Univ., Seoul, Korea.

9.
La Riviere, J. W. M., Kooiman, P., and Schmidt, K. (1967) Kefiran, a novel polysaccharide produced in the kefir grain by Lactobacillus brevis. Arch. Microbiol. 59, 269-278. crossref(new window)

10.
Lim, Y. S. and Lee, S. K. (2009) Characteristics of exopolysaccharide produced in goat milk yogurt cultured with Streptococcus thermophilus LFG isolated from kefir. Korean J. Food Sci. Ani. Resour. 29, 143-150. crossref(new window)

11.
Murofushi, M., Mizuguchi, J., Aibara, K., and Matuhasi, T. (1986) Immunopotentiative effect of polysaccharide from kefir grain, KGF-C, administered orally in mice. Immunopharmacol. 12, 29-35. crossref(new window)

12.
Murofushi, M., Shiomi, M., and Aibara, K. (1983) Effect of orally administered polysaccharide from kefir grain on delayed-type hypersensitivity and tumor growth in mice. Jpn. J. Med. Sci. Biol. 36, 49-53. crossref(new window)

13.
Quirós, A., Hernández-Ledesma, B., Ramos, M., Amigo, L., and Recio, I. (2005) Angiotensin-converting enzyme inhibitory activity of peptides derived from caprine kefir. J. Dairy Sci. 88, 3480-3487. crossref(new window)

14.
Riepenhoff-Talty, M., Offor, E., Klossner, K., Kowalski, E., Carmody, P.J., and Ogra, P.L. (1985) Effect of malnutrition on rotavirus infection in mice. Pediatr. Res. 19, 1250-1253. crossref(new window)

15.
Rodriguez, W. J., Kim, H. W., Arrobrio, J. O., Brandt, C. D., and Chanock, R. M. (1977) Clinical features of acute gastroenteritis associated with human reovirus-like agent in infants and young children. J. Pediatr. 91, 188-193. crossref(new window)

16.
Shiomi, M., Sasaki, K., Murofushi, M., and Aibara, K. (1982) Antirumor activity in mice of orally administered polysaccharide from kefir grain. Jpn. J. Med. Sci. Biol. 35, 75-80. crossref(new window)

17.
Toba, T., Uemura, H., Mukai, T., Itoh, T., and Adachi, S. (1991) A new fermented milk using capsular polysaccharideproducing Lactobacillus kefiranofaciens isolated from kefir grains. J. Dairy Res. 58, 497-502. crossref(new window)

18.
Yokoi, H., Watanabe, T., Fuji, Y., Toba, T., and Adachi, S. (1990) Isolation and characterization of polysaccharide-producing bacteria from kefir grains. J. Dairy Sci. 73, 1684-1689. crossref(new window)