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A Challenging Study to Identify Target Proteins by a Proteomics Approach and Their Validation by Raising Polyclonal Antibody
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 Title & Authors
A Challenging Study to Identify Target Proteins by a Proteomics Approach and Their Validation by Raising Polyclonal Antibody
Jeong, Da-Woon; Park, Beom-Young; Kim, Jin-Hyoung; Hwang, In-Ho;
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This study was conducted to validate the theoretical feasibility of a technique to identify biomarkers in Korean native black pig (KNP) and a commercial Landrace breed. Using two-dimensional electrophoresis, we found six proteins (NADH dehydrogenase Fe-S protein 1, an unnamed protein product, similar to T-complex protein 1, annexin V = CaBP33 isoform, fatty acid-binding protein, and catechol O-methyltransferase), which appeared in KNP alone. We raised polyclonal antibodies (used as the primary antibody) for Western blotting to confirm the characteristics of the six KNP proteins. As a result, catechol O-methyltransferase, annexin V = CaBP33 isoform, and the unnamed protein product presented thicker bands in KNP than those in Landrace. Moreover, catechol O-methyltransferase was shown to be more feasible as a biomarker for KNP. However, cross-reactivity was observed with the polyclonal antibodies for KNP and the other three proteins (NADH dehydrogenase, a protein similar to T-complex protein 1, and fatty acid-binding protein). This study only showed limited results from a limited number of animals; however, our research suggests possibilities for future studies.
Korean native black pig;landrace;proteomics;longissimus dorsi muscle;polyclonal antibody;biomarker;
 Cited by
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