JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Characterization of Anti-Listerial Substance Produced by Lactobacillus salivarius LCH1227
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Characterization of Anti-Listerial Substance Produced by Lactobacillus salivarius LCH1227
Shin, Yu-Ri; Lim, Kong-Boon; Chae, Jong-Pyo; Kang, Dae-Kyung;
  PDF(new window)
 Abstract
In this study, a LCH1227 bacterial strain that possesses anti-listerial activity was isolated from fermented food and identified as Lactobacillus salivarius LCH1227 based on its morphological and biochemical properties, as well as its 16S rRNA gene sequences. Anti-listerial substance also inhibited the growth of various Gram-positive bacteria, such as vancomycinresistant Enterococcus faecalis, Streptococcus agalactiae, Bacillus cereus, Lactobacillus fermentum. The highest level of production of antimicrobial substances from L. salivarius LCH1227 occurred during the early stationary phase. The antilisterial activity was found to be stable over a broad range of pH values (2.0-12.0) and after heat treatment. However, it was inactivated by proteolytic enzymes, indicating its proteinaceous nature. The apparent molecular mass of the partially purified anti-listerial substance, as measured by Tricine-SDS-PAGE, was approximately 5 kDa.
 Keywords
anti-listerial substance;Lactobacillus salivarius;Listeria monocytogenes;
 Language
Korean
 Cited by
1.
Molecular cloning, characterization and comparison of bile salt hydrolases fromLactobacillus johnsoniiPF01, Journal of Applied Microbiology, 2013, 114, 1, 121  crossref(new windwow)
 References
1.
Aasen, I. M., Moretro, T., Katla T., Axelsson, L., and Storro, I. (2000) Influence of complex nutrients, temperature and pH on bacteriocin production by Lactobacillus sakei CCUG 42678. Appl. Microbiol. Biotechnol. 53, 159-166. crossref(new window)

2.
Barrett, E., Hayes, M., O'Connor, P., Gardiner, G., Fitzgerald, G. F., Stanton, C., Ross, R. P., and Hill, C. (2007) Salivaricin P, one of a family of two-component antilisterial bacteriocins produced by intestinal isolates of Lactobacillus salivarius. J. Appl. Bacteriol. 73, 3719-3723.

3.
Cavard, D. and Lazdunski, C. J. (1979) Purification and molecular properties of a new colicin, Eur. J. Biochem., 96, 519. crossref(new window)

4.
Chang, J. Y., Lee, H. H., Kim, I. C., and Chang, H. C. (2001) Characterization of bacteriocin produced by Bacillus licheniformis CY2. J. Korean Soc. Food Sci. Nutr. 30, 410-414.

5.
Cleveland, J., Montville, T. J., Nes, I. F., and Chikindas, M. L. (2001) Bacteriocins: safe, natural antimicrobials for food preservation. Int. J. Food Microbiol. 71, 1-20. crossref(new window)

6.
Daba, H., Panadian, S., Gosselin, J. F., Simard, R., Huang, J., and Lacroix, C. (1991) Detection and activity of a bacteriocin produced by Leuconostoc mesenteroides. Appl. Environ. Microb. 57, 3450-3455.

7.
Daeschel, M. A. and Mckenney, M. C. (1990) Bactericidal activity of Lactobacillus plantarum C11. Food Microbiol. 7, 91-98. crossref(new window)

8.
De Klerk, H. C. and Smit, J. A. (1967) Properties of a Lactobacillus fermenti bacteriocin, J. Gen. Microb. 48, 1309.

9.
Delves-Broughton, J. (1990) Nisin and its uses as a food preservative. Food Technol. 44, 100-117.

10.
De Sad, A. M. S. and De Nadra, M. C. M. (1993) Characterization of bacteriocin produced by Pediococcus pentosaceus from wine. J. Appl. Bacteriol. 74, 406. crossref(new window)

11.
Diez-Gonzalez, F. (2007) Applications of bacteriocins in livestock. Curr. Issues Intestinal Microbiol. 8, 15-24.

12.
Ennahar, S., Sashihara, T., Sonomoto, K., and Ishizaki, A. (2000) Class IIa bacteriocins: biosynthesis, structure and activity. FEMS Microbiol. Rev. 24, 85-106. crossref(new window)

13.
Faber, J. M., Sanders, G. W., and Johnston, M. A. (1989) A survey of various foods for the presence of Listeria species. J. Food Prot. 52, 456-458.

14.
Fredericq, P. (1958) Colicins and colicinogenic facters. Symp. Soc. Exp. Biol. 12, 104.

15.
Galvez, A., Abriouel, H., Lopex, R. L., and Ben Omar, N. (2007) Bacteriocin-based strategies for food biopreservation. Int. J. Microbiol. 30, 51-70.

16.
Gillor, O., Kirkup, B. C., and Riley, M. A. (2004) Colicins and microcins: the next generation antimicrobials. Adv. Appl. Microbiol. 54, 129-146. crossref(new window)

17.
Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., and Williams, S. T. (1994) Regular, nonsporing gram-positive rods. In: Bergey's Manual of Determinative Bacteriology. 9th ed. Williams and Wilkins. Baltimore, USA. pp. 565-570.

18.
Jeoger, M. and Klaenhammer, T. R. (1986) Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 418. J. Bacteriol. 167, 439.

19.
Johnson, J. L., Doyle, M. P., and Cassens, R. G. (1990) Listeria monocytogenes and other Listeria spp. in meat and meat products (a review). J. Food Prot. 53, 81-91.

20.
Karaioannoglou, P. G. and Xenos, G. C. (1980) Survival of Listeria monocytogenes in meatballs. Hell. Vet. Med. 23, 111-117.

21.
Kim, H. T., Park, J. Y., Lee, G. G., and Kim, J. H. (2003) Isolation of a bacteriocin-producing Lactobacillus plantarum strain from Kimchi. Food Sci. Biotechnol. 12, 166-170.

22.
Laura, J. P. and Elmer, H. M. (1990) Listeria monocytogenes threat to a safe food supply; A review. J. Dairy Sci. 37, 912-928.

23.
Lee, H. J., Joo, Y. J., Park, C. S., Kim, S. H., Hwang, I. K., Ahn, J. S., and Mheen, T. I. (1999) Purification and characterization of a bacteriocin produced by Lactococcus lactis subsp. lactis H-559 isolated from Kimchi. J. Biosci. Bioeng. 88, 153-159. crossref(new window)

24.
Lim S. J., Jang, S. S., and Kang, D. K. (2007) Probiotic properties of Lactobacillus salivarius CPM-7 isolated from chicken feces. Kor. J. Microbiol. Biotechnol. 35, 98-103

25.
Liu, W. and Hansen, J. N. (1990) Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis. Appl. Environ. Microb. 56, 2551-2558.

26.
Mayr, H. A., Hedges, A. J., and Berkeley, R. C. W. (1972) Methods for studying bacteriocin. In: Methods in Microbiology, Bergen, T. and Norris, J. R. (eds) Academic Press, New York, pp. 313-342.

27.
Muriana, P. W. and Klaenhammer, T. R. (1991) Clonining, phenotypic expression, and DNA sequence of the gen for lactacin F, a bacteriocin produced by Lactobacillus acidophilus. J. Bacteriol. 55, 1779.

28.
Murry, E. G. D., Webb, R., and Swann, M. B. R. (1926) Disease of rabbits characterized by large mononuclear leucocytosis caused by a hither to undescribed bacterium Bacillus monocytogenes(n. sp.). J. Pat. Bact. 29, 407-439. crossref(new window)

29.
Oravcov, K., Trnckov, T., Kuchta, T., and Kaclkov, E. (2008) Limitation in the detection of Listeria monocytogenes in food in the presence of competing Listeria innocua. J. Appl. Microbiol. 104, 429-37.

30.
Paik, H. D., Koo, K. M., Kim, J. G., and Lee, N. K. (2003) Optimization for lacticin SA72 production by Lactococcus lactis SA72 isolated from Jeot-gal. Kor. J. Microbiol. Biotechnol. 31, 46-50.

31.
Parente, E., Ricciardi, A., and Addario, G. (1994) Influence of pH on growth and bacteriocin production by Lactococcus lactis subsp. lactis 140 NWC during batch fermentation. Appl. Microbiol. Biotechnol. 41, 388-394.

32.
Pavlova, S. I., Kilic, A. O., Kilic, S. S., So, J. S., Nader-Macias, M. E., Simoes, J. A., and Tao, L. (2002) Genetic diversity of vaginal Lactobacillus from women in different countries based on 16S rRNA gene sequences. J. Appl. Microbiol. 92, 451-459. crossref(new window)

33.
Piard, J. C., Delorme, F., Giraffa, G., Commissaire, J., and Desmazeud, M. (1990) Evidence for a bacteriocin produced by Lactococcus lactis CNRZ 481. Neth. Milk Dairy J. 44, 143-158.

34.
Pilasombut, K., Sakpuaram, T., Wajjwalku, W., Nitisinprasert, S., Swetwiwathana, A., Zendo, T., Fujita K., Nakayama, J. and Sonomoto, K. (2006) Purification and amino acid sequence of a bacteriocins produced by Lactobacillus salivarius K7 isolated from chicken intestine. J. Sci. Technol. 28, 121-131.

35.
Ray, S. K., Kim, W. J., Johnson, M. C., and Ray, B. (1989) Conjugal transfer of a plasmid encoding bacteriocin production and immunity in Pediococcus acidilactici H. J. Appl. Bacteriol. 66, 393. crossref(new window)

36.
Ramaswamy, V., Cresence, V. M., Rejitha, J. S., Lekshmi, M. U., Dharsana, K. S., Prasad, S. P., and Vijila, H. M. (2007) Listeria-review of epidemiology and pathogenesis. J. Microbiol. Immunol. Infect. 40, 4-13.

37.
Rammelsberg, M. and Radler, F. (1990) Antibacterial polypeptide of Lactobacillus species. J. Appl. Bacteriol. 69, 177-184. crossref(new window)

38.
Reevs, P. (1965) The bacteriocins. Bacteriol. Rev. 29, 24.

39.
Ruiz-Barba, J. L., Piard, J. C., and Jimenez-Diaz, R. (1991) Plasmid profile and curing of plasmid in Lactobacillus plantarum strains isolated from green olive fermentations. J. Appl. Bacteriol. 71, 417. crossref(new window)

40.
Ryan, M. P., Meaney, W. J., Ross, R. P., and Hill, C. (1998) Evaluation of lacticin 3147 and a teat seal containing this bacteriocin for inhibition of mastitis pathogens. Appl. Environ. Microbiol. 64, 2287-2290.

41.
Schgger, H. and Von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379. crossref(new window)

42.
Sohn, J. H., Lee, J. H., Yi, H., Chun, J., Bae, K. S., Ahn, T. Y., and Kim, S. J. (2004) Kordia algicida gen. no., sp. nov., an algicidal bacterium isolated from red tide. Int. J. Syst. Environ. Microbiol. 54, 675-680. crossref(new window)

43.
Tagg, J. R., Dajani, A. S., and Wannamaker, L. W. (1976) Bacteriocins of gram-positive bacteria. Bacteriol. Rev. 40, 722.

44.
Tagg, J. R. and Mcgiven, A. R. (1971) Assay system for bacteriocin. Appl. Microbiol. 21, 943.

45.
Vasavada, P. C. (1988) Pathogenic bacteria in milk (a review). J. Dairy Sci. 71, 2809-2816. crossref(new window)