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Validation of Korean Meat Products and Processed Cheese for the Detection of GMO using p35S and tNOS Primers
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 Title & Authors
Validation of Korean Meat Products and Processed Cheese for the Detection of GMO using p35S and tNOS Primers
Shin, Hyo-Jin; Heo, Eun-Jeong; Moon, Jin-San; Kim, Ji-Ho; Kim, Young-Jo; Park, Hyun-Jung; Yoon, Yo-Han; Kim, Jin-Man; Wee, Sung-Hwan;
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 Abstract
In this study, 543 samples of press hams, sausages, processed ground meat and processed cheese acquired from retail markets in Seoul and Gyeonggi province in Korea from 2005 to 2010 were monitored using a one-step multiplex polymerase chain reaction (PCR) method that involves the amplification of specific soya or maize endogenous genes and the amplification of 35S promoter (p35S) and nopaline synthase terminator (tNOS) for GMO detection. Among the 543 samples, 477 samples were amplified for maize and/or soybean endogenous genes. Although one sausage sample collected in 2008 showed amplification of tNOS, the result was assumed to be false positive based on the results from further tests of other sausage samples of the same brand. Our results demonstrate the absence of GM soya and/or maze of livestock products in the Korean market during 2005-2010. In addition, the one-step multiplex PCR using previously constructed primer sets appears to be useful as a screening method for the detection of GMOs in processed livestock products. However, more specific methods should be established and employed to detect the event-specific GM gene for positive reaction samples by screening tests in processed livestock products.
 Keywords
livestock products;genetically modified maize;genetically modified soybean;multiplex polymerase chain reaction (PCR);
 Language
English
 Cited by
 References
1.
Abdullah, T., Radu, S., Hassan, Z., and Hashim, J. (2006) Detection of genetically modified soy in processed foods sold commercially in Malaysia by PCR-based method. Food Chem. 98, 575-579. crossref(new window)

2.
Chung, M., Jung, S., Kim, K., Kim, S., Song, S., and Cho, N. (2004) Development and application of polymerase chain reaction methods for detection of the GMO (genetically modified organism) in meat products. Korea J. Vet. Publ. Hlth. 28, 97-107.

3.
Food and Marketing Bureau (2000) Ministry of Agriculture, Forestry and Fisheries of Japan, Tokyo, Japan. Notification No. 1775.

4.
Forte, V., Di Pinto, A., Martino, C., Tantillo, G., Grasso, G., and Schena, F. (2005) A general multiplex-PCR assay for the general detection of genetically modified soya and maize. Food Cont. 16, 535-539. crossref(new window)

5.
Gachet, E., Martin, G., Vigneau, F., and Meyer, G. (1998) Detection of genetically modified organisms (GMOs) by PCR: a brief review of methodologies available. Trends Food Sci. Tech. 9, 380-388. crossref(new window)

6.
Greiner, R., Konietzny, U., and Villavicencio, A. L. C. H. (2005) Qualitative and quantitative detection of genetically modified maize and soy in processed foods sold commercially in Brazil by PCR-based methods. Food Cont. 16, 753-759. crossref(new window)

7.
Holst-Jensen, A., ROnning, S., LOvseth, A., and Berdal, K. (2003) PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem. 375, 985-993.

8.
Hupfer, C., Hotzel, H., Sachse, K., and Engel, K. (1998) Detection of the genetic modification in heat-treated products of Bt maize by polymerase chain reaction. Z. Lebensm. Unters. F A. 206, 203-207. crossref(new window)

9.
Hurst, C., Knight, A., and Bruce, I. (1999) PCR detection of genetically modified soya and maize in foodstuffs. Mol. Breeding 5, 579-586. crossref(new window)

10.
Hwang, S., Lee, C., Nam, Y., Kim, S., Oh, D., and Kim, Y. (2003a) Comparative study of DNA fragment according to steps of genetically modified soybean processed food. J. Food Hyg. Safety 18, 211-217.

11.
Hwang, Y., Kim, H., Kang, T., Lee, J., Lee, I., Shin, D., Kim, S., and Kim, K. (2003b) Physio-ecological characteristics of wild soybeans (Glycine soja) collected throughout Korea and their response to glyphosate. Korea J. Weed Sci. 23, 153-159.

12.
KFDA (2000) Labeling standards for genetically modified foods. KFDA Notification, Korea. No. 2000-43.

13.
Kim, C., Yi, H., Park, S., Yeon, J., Kim, D., Kim, D., Lee, K., Lee, T., Paek, I., and Yoon, W. (2006) Monitoring the occurrence of genetically modified soybean and maize around cultivated fields and at a grain receiving port in Korea. J. Plant Biol. 49, 218-223. crossref(new window)

14.
KOSIS (2005) Statistical Database. Available from: http://www.nso.go.kr/eng/searchable/kosis-list.html.

15.
Kuribara, H., Shindo, Y., Matsuoka, T., Takubo, K., Futo, S., Aoki, N., Hirao, T., Akiyama, H., Goda, Y., and Toyada, M. (2002) Novel reference molecules for quantitation of genetically modified maize and soybean. J. AOAC Int. 85, 1077-1089.

16.
Lee, C., Kim, Y., Hwang, S., and Kang, S. (2004a) Changes in DNA fragments in BT11 corn caused by processing conditions and their monitoring. Korea J. Food Sci. Technol. 36, 299-305.

17.
Lee, S., Park, Y., Kim, J., Park, K., and Kim, Y. (2004b) Qualitative PCR method for detection of genetically modified maize lines NK603 and TC1507. Agric. Chem. Biotechnol. 47, 185-188.

18.
Lin, H., Chiang, J., and Shih, D. (2001) Detection of genetically modified soybeans by PCR method and immunoassay kits. J. Food Drug Anal. 9, 160-166.

19.
MAF (2000) Guideline for labeling of genetically modified agricultural products. Korea MAF notification. No. 2000-31.

20.
Matsuoka, T., Kuribara, H., Akiyama, H., Miura, H., Goda, Y., Kusakabe, Y., Isshiki, K., Toyoda, M., and Hino, A. (2001) A multiplex PCR method of detecting recombinant DNAs from five lines of genetically modified maize. J. Food Hyg. Soc. Jpn. 42, 24-32. crossref(new window)

21.
Meyer, R. (1995) Nachweis gentechnologisch veranderter Pflanzen mittels der Polymerase Kettenreaktion (PCR) am Beispiel derFlavr Savr TM-Tomate. Z. Lebensm. Unters. F A. 201, 583-586. crossref(new window)

22.
Meyer, R., Chardonnens, F., Hubner, P., and Luthy, J. (1996) Polymerase chain reaction (PCR) in the quality and safety assurance of food: detection of soya in processed meat products. Z. Lebensm. Unters. F A. 203, 339-344. crossref(new window)

23.
Miraglia, M., Berdal, K., Brera, C., Corbisier, P., Holst- Jensen, A., Kok, E., Marvin, H., Schimmel, H., Rentsch, J., and Van Rie, J. (2004) Detection and traceability of genetically modified organisms in the food production chain. Food Chem. Toxicol. 42, 1157-1180. crossref(new window)

24.
Phillips, P. and McNeill, H. (2000) A survey of national labeling policies for GM foods. Ag. Bio. Forum. 3, 219-224.

25.
Powell, H., Gooding, C., Garrett, S., Lund, B., and McKee, R. (1994) Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction. Lett. Appl. Microbiol. 18, 59-61. crossref(new window)

26.
Regulation (EC). (2003) No 1829/2003 on genetically modified food and feed. Official J. European Union 268, 10.

27.
Rho, J., Lee, T., Jung, S., Park, Y., and Kim, Y. (2004) Qualitative and quantitative PCR methods for detection of three lines of genetically modified potatoes. J. Agric. Food Chem. 52, 3269-3274. crossref(new window)

28.
Rossen, L., Nørskov, P., Holmstrøm, K., and Rasmussen, O. F. (1992) Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int. J. Food Microbiol. 17, 37-45. crossref(new window)

29.
Tengel, C., Schussler, P., Setzke, E., Balles, J., and Sprenger- Haussels, M. (2001) PCR-based detection of genetically modified soybean and maize in raw and highly processed foodstuffs. Biotech. 31, 426-429.

30.
Wernars, K., Heuvelman, C., Chakraborty, T., and Notermans, S. (1991) Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese. J. Appl. Microbiol. 70, 121-126. crossref(new window)

31.
Wurz, A., Bluth, A., Zeltz, P., Pfeifer, C., and Willmund, R. (1999) Quantitative analysis of genetically modified organisms (GMO) in processed food by PCR-based methods. Food Cont. 10, 385-389. crossref(new window)