JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Immune Modulation Effect of Pig Placenta Extracts in a Mouse Model: Putative Use as a Functional Food Supplement
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Immune Modulation Effect of Pig Placenta Extracts in a Mouse Model: Putative Use as a Functional Food Supplement
Park, Hyun-Jung; Suh, Han-Geuk; Kim, Jin-Hoi; Jang, Ae-Ra; Jung, Hyun-Jung; Lee, Sung-Dae; Ha, Woo-Tae; Lee, Ran; Kim, Ji-Hyuk; Kim, Sang-Ho; Sung, Si-Heung; Moon, Sang-Ho; Kim, Bo-Kyung; Song, Hyuk;
  PDF(new window)
 Abstract
This study was performed to establish an effective extraction method of pig placenta extract that could be used for a putative functional food supplement with immunomodulatory effects. In the present study, we used different temperatures (4, 37, 60, 80, and ) and different solvents (chloroform, NaOH, and phosphate buffered saline [PBS]) to extract the pig placenta. Among the different placenta extracts yielded by the different extraction methods, placenta extract (PE) in PBS at for 30 min (referred to as PE-PBS80) showed a significant increase of nitric oxide production of up to 22.97 cells at a 1 mg/mL dose (p<0.05 ) in J774A.1 cells than other extracts and control tested. Using PE-PBS80, further animal challenges were performed to identify the immune-enhanced effects. As a result, orally administered PE-PBS80 showed a significant increase in blood T and B cell activities and immunoglobulin (IgG and IgM) production. IgG and IgM levels increased to 41.53 mg/mL at a 20 mg dose on day 7 and to 27.38 mg/mL at a 10 mg dose on day 14, respectively (p<0.05). Furthermore, PE-PBS80 was also able to significantly enhance the immune modulator cytokine levels (p<0.05) compared to the control and vehicle treatments. Among the evaluated cytokines, the tumor necrosis factor- (TNF-) level increased to 28.89 pg/mL at extract doses of 20 and 50 mg, the interleukin- (IL-) level increased to 21.52 pg/mL at extract doses of 10, 20, 50 and 75 mg and the interferon (IFN)- level increased to 18.24 pg/mL at extract doses of 10, 20, and 50 mg. Therefore, this study presents an effective method for extracting pig placenta extracts and also demonstrates that pig placenta extracts had significant immunomodulatory effects not only at the cellular level but also in a mouse model, suggesting that this material could be used as an excellent candidate functional food supplement.
 Keywords
placental extract;immune modulator;immunoglobulin;cytokine;temperature;lymphocyte;
 Language
English
 Cited by
1.
Effect of Naturally Derived Substances on Motion Parameters of In Vitro Non-Freezing Preserved Pig Sperm,;;;;;;;;

Reproductive and Developmental Biology, 2013. vol.37. 1, pp.9-16 crossref(new window)
 References
1.
Banerjee, K. K., Bishayee, A., and Chatterjee, M. (1992) Anti-inammatory effect of human placental extract: a biochemical mechanistic approach. Rivista. Europea. per le Scienze Medichee. Farmacologiche. 14, 361-366.

2.
Benedict, C., Scheller, J., Rose-John, S., Born, J., and Marshall, L. (2009) Enhancing influence of intranasal interleukin-6 on slow-wave activity and memory consolidation during sleep. FASEB J. 23, 3629-36. crossref(new window)

3.
Biswas, T. K., Auddy, B., Bhattacharya, N. P., Bhattacharya, S., and Mukherjee, B. (2001) Wound healing activity of human placental extracts in rats. Act. Pharmacol. Sin. 22, 1113-1116.

4.
Bobe, P., Doric, M., Kinsky, R. G., and Voisin, G. A. (1984) Modulation of mouse anti-SRBC antibody response by placental extracts. Cell. Immunol. 89, 355-364. crossref(new window)

5.
Bobe, P., Kanellopoulos-Langevin, C., Bleux, C., and Voisin, G.A. (1986) Modulation of mouse anti-SRBC antibody responses by placental extracts. 11. Antigen specificity and regulatory role of B and T cell populations affected by two distinct placental fractions. J. Immunol. 136, 574-581.

6.
Chatzidakis, I., and Mamalaki, C. (2010) T cells as sources and targets of TNF: implications for immunity and autoimmunity. Curr. Dir. Autoimmun. 11, 105-118. crossref(new window)

7.
Dayer, J., M., Beutler, B., and Cerami, A. J. (1985) Cachetin/tumor necrosis factor stimulates collagenase and prostaglandin E2 production by human synovial cells and dermal fibroblast. Exp. Med. 62, 2163-2168.

8.
Due, H. T., Masse, A., Bode, P., Kinsky, R. G., and Voisin, G. A. (1985) Deviation of humoral and cellular alloimmune reactions by placental extracts. J. Reprod. Immunol. 7, 27-39. crossref(new window)

9.
Fillion, C., Chaouat, G., Reinaud, P., Charpigny, J. C., and Martal J. (1991) Immmoregulatory effects of ovine trophoblast in protein (oTP): all 5 isofoirns suppress PHA-induced lymphocyte proliferation. J. Reprod. Immunol. 19, 237-249. crossref(new window)

10.
Georgieva, R., Stefanov, D., Fichorova, R., Dimitrova, E. (1995) Effects of the whole extract and the chromatographic fractions of the pig placenta on lymphocyte proliferation and humoral immune response. Theriogenology 44, 539-551. crossref(new window)

11.
Green, L. C., Wagner, D. A., Glogowski, J., Skipper, P. L., Wishnok, J. S., Tannenbaum, S. R. (1982) Analysis of nitrate, nitrite, and $[^{15}N]$nitrate in biological fluids. Anal. Biochem. 126, 131-138. crossref(new window)

12.
Gupta, G. S., Kinsky, R. G., Due, H. T., and Voisin, G. A. (1984) Effects of placental extracts on the immune response to histocompatibility antigens: class deviation of alloantibody response and allograft enhancement. Am. J. Reprod. Immunol. 6, 117- 123. crossref(new window)

13.
Gupta, R. and Chattopadhyay, D. (2009) Glutamate is the chemotaxis-inducing factor in placental extracts. Amino Acids 37, 359-366. crossref(new window)

14.
Huang, H., McIntosh, J., and Hoyt, D. G. (2003) An efficient, nonenzymatic method for isolation and culture of murine aortic endothelial cells and their response to inflammatory stimuli. In Vitro Cell Dev. Biol. Anim. 39, 43-50. crossref(new window)

15.
Kamijo, R., Harada, H., Matsuyama, T., Bosland, M., Gerecitano, J., Shapiro, D., Le, J., Koh, S. I., Kimura, T., and Green S. J. (1994) Requirement for transcription factor IRF-1 in NO synthase induction in macrophages. Science 263, 1612-1615. crossref(new window)

16.
Kim, J. H., Lee, J. W., Kim, Y. I., and Lee M. H. (2003) The effect of placental extract on the expression of tyrosinase, TRP-1 and TRP-2 in SK30 melanoma cells. J. Kor. Derma. Assoc. 41, 1612-1618.

17.
Kong, M. H., Lee, E. J., and Lee, S. Y. (2008) Effect of human placental extract on menopausal symptoms, fatigue, and risk factors for cardiovascular disease in middle-aged Korean women. Menopause 15, 296-303. crossref(new window)

18.
Kumar, S., Kanti, B., Liaquat, A., and Biswapati, M. (2003) Anti-inammatory and antiplatelet aggregation activity of human placental extract. Act. Pharmacol. Sini. 24, 187-192.

19.
Liang, W., Huang, Y., Yang, X., Zhou, Z., Pan, A., Qian, B., Huang, C., Chen, J., and Zhang, D. (2006) Oral immunization of mice with plant-derived fimbrial adhesion FaeG induces systemic and mucosal K88ad enterotoxigenic Escherichia coli-specific immune response. FEMS Immunol. Med. Microbiol. 46, 393-399. crossref(new window)

20.
Morikawa, A., Koide, N., Kato, Y., Sugiyama, T., Chakravortty, D., Yoshida, T., and Yokochi, T. (2000) Augmentation of nitric oxide production by gamma interferon in a mouse vascular endothelial cell line and its modulation by tumor necrosis factor alpha and lipopolysaccharide. Infect. Immun. 68, 6209-6214. crossref(new window)

21.
Qu, J. and Thomas, K. (1993) Regulation of inhibin secretion in human placental cell culture by epidermal growth factor, transforming growth factors, and activin. J. Clin. Endocrinol. Metab. 77, 925-931. crossref(new window)

22.
Rosenthal, M. (1982) The application of an extract of human placenta in the treatment of rheumatic affections. Int. J. Tiss. Reac. 4, 147-151.

23.
Skotnicka, B. and Hassman, E. (2008) Proinflammatory and immunoregulatory cytokines in the middle ear effusions. Int. J. Pediatr. Otorhinolaryngol. 72, 13-17. crossref(new window)

24.
Tiwary, S., Shukla, D., Tripathi, A., Agrawal, S., Singh, M., and Shukla, V. (2006) Effect of placental-extract gel and cream on non-healing wounds. J. Wound Care 15, 325-328. crossref(new window)

25.
Togashi, S., Takahashi, N., Iwama, M., Watanabe, S., Tamagawa, K., and Tetsuya, F. (2002) Antioxidative collagenderived peptides in human-placenta extract. Placenta 23, 497-502. crossref(new window)

26.
Uehara, Y., Minowa, O., and Mori, C. (1995) Placental defect and embryonic lethality in mice lacking hepatocyte growth factor/scatter factor. Nature 373, 702 -705. crossref(new window)

27.
Walter, R., Schaffner, A., and Schoedon, G. (1994) Differential regulation of constitutive and inducible nitric oxide production by inflammatory stimuli in murine endothelial cells. Biochem. Biophys. Res. Comm. 202, 450-455. crossref(new window)

28.
Wang, R., Ghahary, A., Shen, Y. J., Scott, P. G., and Tredget, E. E. (1996) Human dermal fibroblasts produce nitric oxide and express both constitutive and inducible nitric oxide synthase isoforms. J. Invest. Dermatol. 106, 419-427. crossref(new window)

29.
Wolf, H. K., Zarnegar, R., and Oliver, L. (1991) Hepatocyte growth factor in human placenta and trophoblastic disease. Am. J. Pathol. 138, 1035-1043.

30.
Wu, J., Yang, T., and Wang, C. (2008) Laennec protects murine from concanavalin A induced liver injury through inhibition of inammatory reactions and hepatocyte apoptosis. Biol. Pharm. Bull. 31, 2040-2044. crossref(new window)