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Development of Analysis Method for Cholesterol in Infant Formula by Direct Saponification
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 Title & Authors
Development of Analysis Method for Cholesterol in Infant Formula by Direct Saponification
Kim, Jin-Man; Park, Jung-Min; Yoon, Tae-Hyung; Leem, Dong-Gil; Yoon, Chang-Yong; Jeong, Ja-Young; Jeong, In-Seek; Kwak, Byung-Man; Ahn, Jang-Hyuk;
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 Abstract
An improved cholesterol analysis method was developed for powdered infant formula by gas chromatographic separation after liquid-liquid extraction and partition. In the official Korea Food Standard method for cholesterol analysis, the water phase and solvent phase were not well separated in the case of emulsified foods such as powdered infant formulas and baby foods. For the rapid and simple sample preparation method, an optimized direct saponification condition was established for heating temperature, heating time, and KOH concentration. From the results, the optimum conditions were as follows: heating temperature , heating time 60 min, and 16 M KOH 10 mL for a 2 g infant formula sample; improved separation condition for gas chromatography was as follows: the initial oven condition was for 25 min, the oven temperature was increased to by /min ratio, and finally the oven temperature remained at for 9 min. The developed method could be implemented for the study of cholesterol, providing the advantages of reduced inspection time and cost in emulsified foods such as infant formula.
 Keywords
cholesterol;direct saponification;gas chromatography;analysis method;infant formula;
 Language
Korean
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