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Effect of Pig Skin Gelatin Hydrolysates on the Bone Mineral Density of Ovariectomized Rats
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 Title & Authors
Effect of Pig Skin Gelatin Hydrolysates on the Bone Mineral Density of Ovariectomized Rats
Park, Jeong-Eun; Ham, Jun-Sang; Kim, Hey-Kyung; Lee, Chi-Ho; Kim, Dong-Wook; Seol, Kuk-Hwan; Oh, Mi-Hwa; Kim, Dong-Hun; Jang, Ae-Ra;
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 Abstract
This study was conducted to examine the effects of low molecular weight gelatin hydrolysates (GH, less than 3kDa), extracted from pig skin collagen on the bone metabolism of ovariectomized (OVX) rats. The rats in the experimental groups were randomly segregated into six different treatment groups such as 1) NC, the normal rat fed AIN 93 diet (basal diet) only; 2) OC, the OVX rat fed the basal diet only; 3) GH 0.1, the OVX rat fed the basal diet with 0.1% GH; 4) GH 0.8, the OVX rat fed the basal diet with 0.8% GH; 5) G 0.1, the OVX rat fed the basal diet with 0.1% gelatin; 6) G 0.8, the OVX rat fed the basal diet with 0.8% gelatin. Body weight gain in the GH 0.1, GH 0.8, and G 0.8 was significantly higher than those in the NC and OC. Feed intake of the GH 0.1 and GH 0.8 was higher than that of the NC and OC, while no significant difference was found in feed efficiency ratio (FER). BMD of the GH 0.8 was higher than that of the OC. However, gelatin hydrolysates and gelatin resulted in higher BMC level compare to the OC. Serum HDL-cholesterol of rat fed GH and gelatin was higher than that of OC (p<0.05). LDL-C of the GH 0.1 and the GH 0.8 tended to be less than that of OC. Serum alkaline phosphatase (ALP) of the GH 0.1 was lower than that of the OC. The serum of GH 0.8 showed lower osteocalcin value than the OC (p<0.05). In addition, GOT and GPT levels significantly decreased in all treatment groups. These results indicated that gelatin hydrolysates from pig skin gelatin hydrolysates enhanced BMD and serum biochemical parameters related to bone metabolism. Therefore, the gelatin hydrolysates could be used as a beneficial material to improve bone health.
 Keywords
gelatin;collagen;osteoporosis;bone metabolism;pig skin;
 Language
Korean
 Cited by
1.
멸치(Engraulis japonicus)젓 잔사 분말칼슘이 난소적출 흰쥐의 골다공증에 미치는 영향,문지영;조은지;주성제;박종훈;윤성호;김만도;김영만;현숙경;임은서;김태훈;손병일;권현주;김병우;이은우;

한국수산과학회지, 2015. vol.48. 4, pp.426-431 crossref(new window)
1.
Effect of Calcium Extracted from Salt-fermented Anchovy Engraulis japonicus on Osteoporosis in Ovariectomized SD-Rats, Korean Journal of Fisheries and Aquatic Sciences, 2015, 48, 4, 426  crossref(new windwow)
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In vitro responses of hFOB1.19 cells towards chum salmon (Oncorhynchus keta) skin gelatin hydrolysates in cell proliferation, cycle progression and apoptosis, Journal of Functional Foods, 2013, 5, 1, 279  crossref(new windwow)
 References
1.
Anderson, J. J. B. (1999) Plant-based diets and bone health : nutritional implications. Am. J. Clin. Nutr. 70, 593-42.

2.
Chung, H. Y. (2008) Osteoporosis diagnosis and treatment 2007. Korean J. Soc. End. 23, 76-108. crossref(new window)

3.
Duda, R. J. Jr., O'Brien, J. F., Katzmann, J. A., Peterson, J. M., Mann, K. G., and Riggs, B .L. (1988) Concurrent assays of circulating bone Gla-protein and bone alkaline phosphatase : effects of sex, age, and metabolic bone disease. J. Clin. Endocrinol. Metab. 66, 951-957. crossref(new window)

4.
Friedewald, W. T., Ley, R. I., and Fredrickson, D. S. (1972) Estimation of the concentration of low density lipoprotein cholesterol the preparative ultracentrifuge. Clin. Chem. 18, 499-502.

5.
Guillerminet, F., Beaupied, H., Fabien-Soule, V., Tome, D., Benhamou, C-L., Roux, C., and Blais, A. (2010) Hydrolyzed collagen improves bone metabolism and biomechanical parameters in ovariectomized mice : An in vitro and in vivo study. Bone 46, 827-834. crossref(new window)

6.
Jahng, J. S. (1994) Prevention and treatment of the osteoporotic fracture. Korean J. Soc. Bone Metab. 1, 147-155.

7.
Jerome, S. P., Gabrielle, L., and Raul, F. (1998) Identification of collagen fibrils in scleroderma skin. J. Invest. Dermatol. 90, 48-54.

8.
Kanis, J. A., Melton, L. J., Christiansen, C., Johnston, C. C., and Khaltaev, N. (1994) The diagnosis of osteoporosis. J. Bone. Min. Res. 9, 1137-1141.

9.
Kim, G. H., Jeon, Y. J., Byun, H. G., Lee, Y. S., Lee, E. H., and Kim, S. K. (1998) Effect of calcium compounds from oyster shell bound fish skin gelatin peptide in calcium deficient rats. Korean J. Fish. Soc. 32, 149-159

10.
Kim, H. S., Yoon, H. D., Seong, J. H., Lee, Y. G., Xie, C. L., Kim, S. H., and Choi, W. S. (2009) Effects of soluble collagen peptides extract derived from Mugil cephalus scale on the blood glucose and lipid metabolism in diabetic rats. Korean J. Life Sci. 19, 1794-1801. crossref(new window)

11.
Kim, J. W., Kim, D. K., Kim, M. J., and Kim, S. D. (2010) Extraction and bleaching of acid-and pepsin-soluble collagens from shark skin and muscle. Korean J. Food Preserv. 17, 91-99.

12.
Kim, S. J. (2003) Study on the relationship between osteoporosis-cause factor and bone mineral density, Biochemical Marker. Pukyong National University.

13.
Koo, Y. M., Kim, S. H., Kim, E. Y., Lee, H. S., Choi, H., Sohn, Y. J., Jung, H. S., and Sohn, N. W. (2008) Effects of Lycii Fructus on the ovariectomized osteoporosis of rats. Korean J. Oriental Med. 29, 144-154.

14.
Kwon, M. C., Kim, C. H., Kim, H. S., Abdul Qadir Syed., Hwang, B. Y., and Lee, H. Y. (2007) Anti-wrinkle activity of low molecular weight peptides derived. Korean J. Food Sci. Technol. 39, 625-629.

15.
Lane, J. M. and Nydick, M. (1999) Osteoporosis: Current modes of prevention and treatment. Am. J. Acad. Orthop. Surg. 7, 19-31.

16.
Lee, H. J. (2002) Review of biochemical bone metabolism marker's change in osteoporosis incidence factors. Korean J. Soc. Phys. Therapy 14, 213-220.

17.
Lee, J. W., Kim, H. J., Jhee, O. H., Won, H. D., Yu, Y. J., Lee, M. H., Kim, T. H., Om, A. S., and Kang, J. S. (2005) Effects of alternative medicine extract on bone mineral density, bone strength and biochemical markers of bone metabolism in ovariectomized rats. Korean J. Food Nutr. 18, 72-80.

18.
Lee, Y. A. and Kim, M. H. (2008) Effects of sea tangle extract on formation of collagen and collagen cross-link in ovariectomized rats. Korean J. Life Sci. 18, 1578-1583. crossref(new window)

19.
Lee, Y. B., Lee, H. J., Kim, K. S., Lee, J. Y., Nam, S. Y., Cheon, S. H., and Shon, H. S. (2004) Evaluation of the preventive effects of isoflavone extract on bone loss in ovariectomized rats. Biosci. Biotechnol. Biochem. 68, 1040-1045. crossref(new window)

20.
Liu, D., Liang, L., Regenstein, J. M., and Zhou, P. (2012) Extraction and characterization of pepsin-solubilised collagen from fins, scales, skins, bones and swim bladders of bighead carp (Hypophthalmichthys nobilis). Food Chem. Doi:10.1016/j.foodchem.2012.02.032. crossref(new window)

21.
Liu, G. and Peacock, M. (1998) Age-related changes in serum undercarboxylated osteocalcin and its relationships with bone density, bone quality, and hip fracture. Calcif. Tissue Int. 62, 286-289. crossref(new window)

22.
Maeda, M., Tani, S., Sano, A., and Fujioka, K. (1999) Microstructure and release characteristics of the mini pellet, a collagen-based drug delivery system for controlled release of protein drugs. J. Con. Release 62, 313-324. crossref(new window)

23.
Nomura, Y., Oohashi, K., Watanabe, M., and Kasugai, S. (2005) Increase in bone mineral density through oral administration of shark gelatin to ovariectomized rats. Nutrition 21, 1120-1126. crossref(new window)

24.
Nordin, B. E. C., Wishart, J. M., Clifton, P. M., McArthur, R., Scopacasa, F., Need, A. G., Morris, H. A., O'Loughlin, PD., and Horowitz, M. (2004) A longitudinal study of bonerelated biochemical changes at the menopause. J. Clin. Endocrinol. 61, 123-130. crossref(new window)

25.
Notelovitz, M. 1993. Osteoporosis: screening, prevention, and management. Fert. Ster. 59, 707-725.

26.
Oh, K. W. (2008) Diabetes and osteoporosis. Korean J. Bone Metab. 15, 91-98.

27.
Park, S. S., Lee, H. J., Yoon, W. J., Kang, G. J., Yang, E. J., Kim, H. S., Choo, C. S., Kang, H. K., and Yoo, E. S. (2010) Effects of horse bone extracts on the induced postmenopausal osteoporosis in rats. Korean J. Pharmacogn. 41, 204-209.

28.
Park, Y. H., Yoon, S., Chung, S. Y., Yang, S. O., Yoo, T. M., Yang, J. S., and Kwon, D. J. (2001) The effect of isoflavone supplementation on bone metabolism in ovariectomized SD rats. Korean J. Food Sci. Nutr. 30, 657-666.

29.
Pei, M., Yu, C., and Qu, M. (2000) Expression of collagen type I, II, and III in loose body of osteoarthritis. J. Orthop. Sci. 5, 288-293. crossref(new window)

30.
Price, P. A., Parthemore, J. G., and Deftos, L. J. (1980) New biochemical marker for bone metabolism. Measurement by radioimmunoassay of bone GLA protein in the plasma of normal subjects and patients with bone disease. J. Clin. Invest. 66, 878-883. crossref(new window)

31.
Seguro, K. and Motoki, M. (1990) Functional properties of enzymatically phosphorylated soybean proteins. Agric. Biol. Chem. 54, 1271-1276. crossref(new window)

32.
Wronski, L. (1995) Response of femoral neck to estrogen depletion and parathyroid hormone in age rats. J. Bone. 16, 551-557. crossref(new window)

33.
Yook, T. H., Bae, J. S., Kim, Y. J., Kim, D. K., Jang, I. K., and Lee, C. H. (2006) Effects of cervi pontotrichum cornu and Carthami semen on the experimental osteoporosis induced by ovariectomy in rats. Korean J. Oriental Phys Path. 20, 1226-1232.