Advanced SearchSearch Tips
Effects of Egg Shell Membrane Hydrolysates on Skin Whitening, Wound Healing, and UV-Protection
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Egg Shell Membrane Hydrolysates on Skin Whitening, Wound Healing, and UV-Protection
Park, Ki-Moon; Yoo, Jin-Hee; Shin, Young-Jae;
  PDF(new window)
This study was conducted to examine the effects of egg shell membrane hydrolysates (ESMH) on skin whitening, wound healing, and UV-protection. ESMH was divided into three groups by molecular weight (Fraction I: above 10 kDa of ESMH, Fraction II: 3 kDa-10 kDa of ESMH, Fraction III: below 3 kDa of ESMH). As a result, all of ESMHs showed over 90% of protein contents. The wound healing experiment using HaCaT cells showed that the fraction I was slightly superior to other fractions depending on the concentration though it was not significantly different. In the experiments of inhibition of tyrosinase and L-3,4-dihydroxyphenylalanine (L-DOPA) oxidation to verify the L-DOPA whitening effect, the whole ESMH (before fractioning) showed a similar amount of inhibition effect with arbutin (control). In the inhibition of melanin formation in B16-F1 melanoma cells, the fraction I showed a high inhibitory effect. In the experiment for protecting the skin from ultraviolet rays using HaCaT cells, all the fractions showed a higher rate of cell viability than the control. In conclusion, this study confirmed that the cosmetic effects of ESMHs such as skin whitening, wound healing, and UV-protection, which were divided depending on the molecule weight. We could confirm that the possibility of ESMHs as a material for functional cosmetics.
egg shell membrane;skin whitening;wound healing;UV-protection;
 Cited by
Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection,Yoo, Jinhee;Park, Kimoon;Yoo, Youngji;Kim, Jongkeun;Yang, Heejin;Shin, Youngjae;

한국축산식품학회지, 2014. vol.34. 1, pp.26-32 crossref(new window)
Effects of Egg Shell Membrane Hydrolysates on UVB-radiation-induced Wrinkle Formation in SKH-1 Hairless Mice, Korean Journal for Food Science of Animal Resources, 2015, 35, 1, 58  crossref(new windwow)
Preparation of copper-containing bioactive glass/eggshell membrane nanocomposites for improving angiogenesis, antibacterial activity and wound healing, Acta Biomaterialia, 2016, 36, 254  crossref(new windwow)
Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection, Korean Journal for Food Science of Animal Resources, 2014, 34, 1, 26  crossref(new windwow)
Arias, J. L., Fernandez, M. S., Dennis, J. E., and Caplan, A. I. (1991) Collgens of the chicken egg shell membrane. Connect. Tissue Res. 26, 37-43. crossref(new window)

Bollag, D. M. and Edelstein, S. J. (1991) Protein methods. Wiley-liss, New York, pp. 56-59.

Borski, R. J. (2000) Nongenomic membrane actions of glucocorticoids in vertebrates. Trends Endocrinol. Metab. 11, 427-436. crossref(new window)

Candilish, J. K. and Scougall, R. K. (1969) L-5-hydroxylysine as a constituent of the shell membrane of the hen's egg. Int. J. Protein Res. 1, 299-306.

Carmichael, J., Mitchell, J. B., DeGraff, W. G., Gamson, J., Gazdar, A. F., Johnson, B. E., Glatstein, E., and Minna, J. D. (1988) Chemosensitivity testing of human lung cancer cell lines using the MTT assay. Br. J. Cancer 57, 540-547. crossref(new window)

Daniel, F., Randall, L., Adams, G., Paul, D., Marshall, D., and Franklin, R. (2008) Therapeutic, nutraceutical and cosmetic applications for eggshell membrane and processed eggshell membrane preparations. US patent 20,080,234,195.

Gonzaga, E. R. (2009) Role of UV light in photodamage, skin aging, and skin cancer: importance of photo protection. Am. J .Clin. Dermatol. 1, 9-24.

Gurtner, G. C., Werner, S., Barrandon, Y., and Longaker, M. T. (2008) Wound repair and regeneration. Nature 453, 314- 321. crossref(new window)

Held, E., Sveinsdottir, S., and Agner, T. (1999) Effect of long term use of moisturizer on skin hydration, barrier function and susceptibility to irritants. Acta Dermatol. Veneresol. 79, 49. crossref(new window)

Hosgood, G. (2003) Wound repair and specific tissue response to injury. Saunders, Philadelphia, pp. 66-86.

Ishihara, Y., Oka, M., Tsunakawa, M., Tomita, K., Hatori, M., Yamamoto, H., Kamei, H., Miyaki, T., Konishi, M., and Oki, T. (1991) Melanostatin, a new melanin synthesis inhibitor. Production, isolation, chemical properties, structure and biological activity. J. Antibiot. 44, 25-32. crossref(new window)

Jeon, S. H., Kim, K. H., Koh, J. U., and Kong, K. H. (2005) Inhibitory effects on L-Dopa oxidation of tyrosinase by skinwhitening agents. Bull. Korean Chem. 26, 1135-1137. crossref(new window)

Kang, H. H. (1997) Anti-aging in cosmetics. J. Soc. Cosmet. Scientists Korea 23, 57-73.

Karna, E., Nazaruk, J., Szoka, L., and Palka, J. A. (2011) Scutellarin-dependent inhibition of collagen biosynthesis in cultured fibroblasts. Nat. Prod. Res. 25, 1789-1795. crossref(new window)

KOSIS (2011) Korean Statistical Information Service, Korea.

Leach, R. M., Rucker, R. B., and Dyke, G. P. V. (1981) Egg shell membrane protein : A non elastin desmosine/ iso-desmosine containing protein, Arch. Biochem. Biophys. 207, 353-359. crossref(new window)

Marrot, L. and Meunier, J. R. (2008) Skin DNA photo damage and its biological consequences. J. Am. Acad. Dermatol. 58, 139-148. crossref(new window)

Matsumoto, H., Ohara, H., Itoh, K., Nakamura, Y., and Takahashi, S. (2006) Clinical effects of fish type I collagen hydrolysate on skin properties. ITE Lett. 7, 386-390.

Meyskens, F. L. and Fuller, B. B. (1980) Characterization of the effects of different retinoids on the growth and differentiation of a human melanoma cell line and selected subelines. Cancer Res. 40, 2194.

Mishima, Y., Hatta, S., Ohyama, Y., and Inazu, M. (1988) Induction of melanogenesis suppression: cellular pharmacology and mode of differential action. Pigment Cell Res. 1,367-374. crossref(new window)

Morimura, S., Nagata, H., Uemura, Y., Fahmi, A., Shigematsu, T., and Kida, K. (2002) Development of an effective process for utilization of collagen from livestock and fish waste. Process Biochem. 37, 1403-1412. crossref(new window)

Nakagawa, K., Sakurai, K., Ueda, K. Y., and Ueda, T. (2011) Age-related changes in elastic properties and moisture content of lower labial mucosa. J. Oral Rehabil. 38, 235-241. crossref(new window)

Oikarinen, A., Karvonen, J., Uitto, J., and Hannuksela, M. (1985) Connective tissue alterations in skin exposed to natural and therapeutic UV-radiation. Photodermatol. 2, 15-26.

Okubo, T., Akachi, S., and Hatta, H. (1997) Structure of hen eggs and physiology of egg laying. Hen eggs 1-12.

Park, J. G., Hyun, J. W., Lim, K. H., Shin, J. E., Won, Y. J., Yi, Y. D., Shin, K. H., Chang, I. M., and Woo, W. S. (1993) Antineoplastic effects from traditional medicinal plants. Kor. J. Pharmacogn. 24, 223-230.

Pawelek, J. M. and Korner, A. M. (1982) Biosynthesis of mammalian melanin. Am. Sci. 70, 136-145.

Vignardet, C., Guillaumeb, Y. C., and Michela, L. (2001) Comparison of two hard keratinous substrates submitted to the action of a keratinase using an experimental design. Intl. J. Pharm. 224, 115-122. crossref(new window)

Xiang, L., Chung, G. L., Ellen, Y. S. C., and Jason, C. H. S. (1992) Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl. Environl. Microbiol. 58, 3271-3275.

Yada, Y., Higuchi, K., and Imokawa, G. (1991) Effects of endothelins on signal transduction and proliferation in human melanocytes. J. Biol. Chem. 266, 18352-18357.

You, J. K., Chung, M. J., Kim, D. J., Deo, D. J., Park, J. H., Kim, T. W., and Choe, M. (2009) Antioxidant and tyrosinase inhibitory effects of Paeonia suffruticosa water extract. J. Korean Soc. Food Sci. Nutr. 38, 292-296. crossref(new window)