Advanced SearchSearch Tips
Immunological Activity of Bovine Colostral Whey Protein Containing TGF-β from Imsil Province
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Immunological Activity of Bovine Colostral Whey Protein Containing TGF-β from Imsil Province
Yang, Hee-Sun; Oh, Hyun-Hee; Choi, Hee-Young; Park, Jong-Hyuk; Kim, Kyoung-Hee; Oh, Jeon-Hui; Jung, Hoo-Kil;
  PDF(new window)
This experiment was carried out in order to separate bovine colostral whey protein from Imsil province and to test the effect of immunological activity on RAW 264.7 cells. The colostral whey protein contained TGF- 7, 475 pg/g in total. We first tested the effect of the colostral whey protein on the proliferation of RAW 264.7 cells and it demonstrated cytotoxicity at concentrations greater than 20 mg/mL. Therefore, the immunological activities of colostral whey protein were investigated in maximum concentration of 10 mg/mL on LPS-induced RAW 264.7 cells. Results indicated that colostral whey protein inhibited the LPS-induced nitric oxide (NO) production in a dose-dependent manner. The colostral whey protein also suppressed the productions of proinflammatory cytokines (TNF-, IL-, IL-6) in a dose-dependent manner. In addition to the immunological activity, colostral whey protein led to the expression of heme oxygenase-1 (HO-1) in RAW 264.7 cells. In conclusion, colostral whey protein containing TGF- inhibited the production of NO, TNF-, IL-, and IL-6 via expression of HO-1.
colostrum;transforming growth factor-;immunological activity;
 Cited by
Ahn, J. H. and Park J. K. (2010) Effect of supplementation of fermented colostrum on growth and occurrence of diarrhea in holstein calves. J. Ani. Sci. Technol. 52, 281-286. crossref(new window)

An, M. J., Cheon, J. H., Kim, S. W., Park, J. J., Moon, C. M., Han, S. Y., Kim, E. S., Kim, T. I., and Kim, W. M. (2009) Bovine colostrums inhibits nuclear factor kB-mediated proinflammatory cytokine expression in intestinal epithelial cells. Nutr. Res. 29, 275-280. crossref(new window)

Choi, H. S., Jung, K. H., Lee, S. C., Yim, S. V., Chung, J. H., Kim, Y. W., Jeon, W. K., Hong H. P., Ko, Y. G., Kim, C. H., Jang, K. H., and Kang, S. A. (2009) Bovine colostrum prevents bacterial translocation in an intestinal ischemia/reperfusion- injured rat model. J. Med. Food. 12, 37-46. crossref(new window)

Elfstrand, L., Lindmak-Mnsson, H., Paulsson, M., Nyberg, L., and kesson, B. (2002) Immunoglobulins, growth factors and growth hormone in bovine colostrums and the effects of processing. Int. Dairy J. 12, 879-887. crossref(new window)

Jeon, S. K. (2001) Characterization of TGF-$\beta$ present in human and bovine colostrum. M.D. Thesis, Kangwon Univ., Chuncheon, Korea.

Jeong, I. Y., Jin, C. H., Pack, Y. D., Lee, H. J., Choi, D. S., Byun, M. W., and Kim, Y. J. (2008) Anti-inflammatory activity of an ethanol extract of Caesalpinia sappan L. in LPSinduced RAW 264.7 cells. J. Food Sci. Nutr. 13, 253-258. crossref(new window)

Jin, M., Suh, S. J., Yang, J. H., Lu, Y., Kim, S. J., Kwon, S., Jo, T. H., Kim, J. W., Park, Y. I., Ahn, G. W., Lee, C. K., Kim,C. H., Son, J. K., Son, K. H., and Chang, H. W. (2010) Antiinflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW 264.7 cells via NF-${\kappa}B$ and ERK1/2 inactivation. Food Chem. Toxicol. 48, 3073-3079. crossref(new window)

Kang, S. H. (2007) Milk-derived growth factors as neutraceuticals. Korean J. Dairy Sci. Technol. 25, 27-31.

Kim, J. W., Jeon, W. K., and Kim, E. J. (2005) Combined effects of bovine colostrum and glutamine in diclofenacinduced bacterial translocation in rat. Clin. Nutr. 24, 785- 793. crossref(new window)

Koo, G. C., Manyak, C. L., Dasch, J., Ellingsworth, L., and Shultz, L. D. (1991) Suppressive effects of monocytic cells and transforming growth factor-beta on natural killer cell differentiation in autoimmune viable motheaten mutant mice. J. Immunol. 147, 1197-1200.

Kwon, O. H., Lee, J. S., Choi, H. S., Hong, H. P., Jang, K. H., Park, J. H., Kang, S. A., and Ko, Y. G. (2010) Antioxidant and anfticytokine effects of abovine colostrum in intestinal ischemia/reperfusion injured rat motel. Food Sci. Biotechnol. 19, 1295-1301. crossref(new window)

Liblau, R. S., Singer, S. M., and McDevitt, H. O. (1995) Th1 and Th2 cells in the pathogenesis of organ-specific autoimmune disease. Immunol. Today 16, 34-38. crossref(new window)

Matsuno, R., Aramaki, Y., and Tsuchiya, S. (2001) Involvement of TGF-$\beta$ in inhibitory effects of negatively charged liposomes on nitric oxide production by macrophages stimulated with LPS. Biochem. Biophys. Res. Commun. 281, 614- 620. crossref(new window)

Mitani, T., Terashima, M., Yoshimura, H., Nariai, Y., and Tanigawa, Y. (2005) TGF-${\beta}1 $ enhances degradation of IFN- $\gamma$-induced iNOS protein via proteasomes in RAW 264.7 cells. Nitric Oxide 13, 78-87. crossref(new window)

Nam, M. S., Bae, H. C., Kim, P. H., Kim, W. S., and Goh, J. S. (2002) Purification of TGF-${\beta}1$ from bovine colostrums. Korean J. Food Sci. Ani. Resour. 22, 343-347.

Pakkanen, R. and Aalto, J. (1997) Growth factors and antimicrobial factors of bovine colostrums. Int. Dairy J. 5, 285- 297.

Park, J. D., Son, D. H., Jeong, K. S. (2003) Stability of immunoglobulin G(IgG) by heat treatment. Korean J. Food Preserv. 10, 236-240.

Playford, R. J., Floyd, D. N., MacDonald, C. E., Calnan, D. P., Adenekan, R. O., Johnson, W., Goodlad, R. A., and Marchbank, T. (1999) Bovine colostrums is health food supplement which prevents NSAID induced gut damage. Gut 44, 653- 658. crossref(new window)

Rodriguez, C., Castro, N., Capote, J., Morales-delaNuez, A., Moreno-Indias, I., Sanchez-Macias, D., and Arguello, A. (2008) Effect of colostrum immunoglobulin concentration on immunity in Majorera goat kids. J. Dairy Sci. 92, 1696-1701.

Simmen, F. A., Cera, K. R., and Maha, D. C. (1990) Stimulation by colostrums or mature milk of gastrointestinal tissue development in newborn pigs. J. Ani. Sci. 68, 3596-3603. crossref(new window)

Srisook, K., Palachot, M., Mongkol, N., Srisook, E., and Sarapusit, S. (2011) Anti-inflammatory effect of ethyl acetate extract from Cissus quadrangularis Linn may be involved with induction of heme oxygenase-1 and suppression of NF-${\kappa}B$ activation. J. Ethnopharm. 133, 1008-1014. crossref(new window)

Su, H. C., Leite-Morris, K. A., Braun, L., and Biron, C. A. (1991) A role for transforming growth factor-${\beta}1$ in regulating natural killer cells and T lymphocytes proliferative responses during acute infection with lymphocytic choriomeningitis virus. J. Immunol. 147, 2712-2727.

Sugisawa, H., Itou, T., and Sakai, T. (2001) Promoting effect of colostrums on the phagocytic activity of bovine polymorphonuclear leukocytes in vitro. Biol. Neonate 79, 140-144. crossref(new window)

Teintenier-Cousin, C., Lefranc-Millot, C., Froidevaux, G., Slomianny, M., Guillochon, D., and Vetcigne, D. (2009) Preparation from bovine colostrum of a fraction rich in latent transforming growth factor-${\beta}2$ and cleared of most allergenic proteins by one-step Cibacron Blue chromatography. Int. Dairy J. 19, 286-294. crossref(new window)

Uruakpa, F. O., Ismond, M. A. H., and Akoboundu, E. N. T. (2002) Colostrum and its benefits: a review. Nutr. Res. 22, 755-767. crossref(new window)

Yang, H. J., and Lee, S. W. (2006) Antimicrobial activities of lactoferrin and its hydrolysate obtained the colostrums of hanwoo and Holstein cattle. J. Anim. Sci. Technol. 48, 595- 602. crossref(new window)