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Determination of Lactulose and Furosine Formation in Heated Milk as a Milk Quality Indicator
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 Title & Authors
Determination of Lactulose and Furosine Formation in Heated Milk as a Milk Quality Indicator
Cho, Young-Hee; Hong, Sung-Moon; Kim, Cheol-Hyun;
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During heat treatment and storage of milk, deteriorative reaction takes place, which consequently influence on the milk quality. In this study, formation of lactulose and furosine under different thermal conditions and storage conditions, and the ratio of lactulose and furosine (LU/FU) in presence of reconstituted milk powder were determined to establish chemical indicators for heat damages of milk and the adulteration of fresh milk in dairy field. The lactulose and furosine contents linearly increased with increased heating temperature and heating time. It showed high correlation between the formation of lactulose and furosine, and the treatment temperature and time (p<0.05). The lactulose and furosine concentration of HTST milk and UHT milk noticeably increased during storage at , but there was no noticeable increase of lactulose and furosine concentration at lower storage temperature. In the raw milk, the lactulose and furosine contents greatly increased with the addition of reconstituted milk. The increase level of furosine was much higher than that of lactulose, which consequently resulted in the lower LU/FU ratio in milk as increase of added reconstituted milk amounts. As comparing with raw milk, there was more than twice reduction in LU/FU ratios after the addition of reconstituted milk (p<0.05). It can be concluded that lactulose and furosine are suitable milk quality indicators of heat damage and for demonstrating improper addition of reconstituted milk powder.
lactulose;furosine;chemical indicator;milk quality;
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The influence of ultra-pasteurization by indirect heating versus direct steam injection on skim and 2% fat milks, Journal of Dairy Science, 2017, 100, 3, 1688  crossref(new windwow)
Akalin, A. S. and Gonc, S. (1997) Lactulose and 5-HMF contents in market milks. Milchwissenschaft 52, 377-379.

Andrews, G. (1984) Distinguishing pasteurized, UHT and sterilized milks by their lactulose content. J. Soc. Dairy Tech. 37, 92-95. crossref(new window)

Corzo, N., Delgado, T., Troyano, E., and Olano, A. (1994) Ratio of lactulose to furosine as indicator of quality of commercial milks. J. Food Prot. 57, 737-739.

De Block, J., Merchiers, M., Van Renterghem, R., and Moermans, R. (1996) Evaluation of two methods for the determination of lactulose in milk. Int. Dairy J. 6, 217-222. crossref(new window)

De Rafael, D., Villamiel, M., and Olano, A. (1997) Formation of lactulose and furosine during heat treatment of milk at temperatures of 100-120${^{\circ}C}$. Milchwissenschaft 52, 76-78.

Delgado, T., Corzo, N., Santa-Maria, G., Jimeno, M. L., and Olano. A. (1992) Determination of furosine in milk samples by ion-pair reversed phase liquid chromatography. Chromatographia 33, 374-376. crossref(new window)

Guerra-Hernandez, E., and Corzo, N. (1996) Furosine determination in baby cereals by ion-pair reversed-phase liquid chromatography. Anal. Tech. Instrument. 73, 729-731.

Guerra-Hernandez, E., Corzo, N., and Garcia-Villannova, B. (1999) Maillard reaction evaluation by furosine determination during infant cereal processing. J. Cereal Sci. 29, 171-176. crossref(new window)

Guerra-Hernandez, E., Leon, C., Corzo, N., Garcia-Villannova, B., and Romera, J. M. (2002) Chemical changes in powdered infant formulas during storage. Int. J. Dairy Technol. 55, 171-176. crossref(new window)

Hewedy, M., Kiesner, M. C., Meissner, K., Hartkopf, J., and Erbersdobler, H. F. (1994) Effects of UHT heating of milk in an experimental plant on several indicators of heat treatment. J. Dairy. Res. 61, 305-308. crossref(new window)

Hurrell, R. F., and Finot, P. A. (1983) Storage of milk powders under adverse conditions. Br. J. Nutr. 49, 343-354. crossref(new window)

IDF (1991) Heat treated milk Determination of lactulose content: High performance liquid chromatography (reference method) Bulletin of the International Dairy federation 147/1991, Brussels, pp. 621-623

IDF (1992) Influence of technology on the quality of heat treated milk and fluid milk products. Bulletin of the International Dairy federation, B-Doc. 222, Brussels.

Kulmyrzaev, A. and Dufour, E. (2002) Determination of lactulose and furosine in milk using front-face fluorescence spectroscopy. Lait 82, 725-735 crossref(new window)

Lan, X. Y., Wang, J. Q., BU, D. P., Shen, J. S., Zheng, N., and Sun, P. (2010) Effects of heating temperatures and addition of reconstituted milk on the heat indicators in milk. J. Food Sci. 75, C653-C658. crossref(new window)

Marconi, E., Messia, M.C., Amine, A., Moscone, D., Vernazza, F., Stocchi, F., and Palleschi, G. (2004) Heat-treated milk differentiation by a sensitive lactulose assay. Food Chem. 84, 447-450. crossref(new window)

Montilla, A., Calvo, M., Santa-maria, G., Gorzo, N., and Olano, A. (1996) Correlation between lactulose and furosine in UHT-heated milk. J. Food Prot. 59, 1061-1064.

Montilla, A. and Olano, A. (1997) Effect of the protein content and dilution on the lactulose/furosine ratio in heat-treated milk. Milchwissenschaft 52, 506-507.

Mortier, L., Braekman, A., Caltuyvels, D., Van Renterghem, R., and De block, J. (2000) Intrinsic indicators for monitoring heat damage of consumption milk. Biotechnol. Agron. Soc. Environ. 4, 221-225.

Pellegrino, L. (1994) Influence of fat content on some heatinduced changes in milk and cream. Neth. Milk Dairy J. 48, 71-80.

Pellegrino, L., De Noni, I., and Resmini, P. (1995) Coupling of lactulose and furosine indices for quality evaluation of sterilized milk. Int. Dairy J. 5, 647-659. crossref(new window)

Resmini, P. and Pellegrino, L. (1994) HPLC of furosine in for evaluating Maillard reaction damage in skimmilk powders during processing and storage. Bulletin of the International Dairy federation 298/1994. Brussels, pp. 31-36.