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Effect of NaCl on Thermal Resistance, Antibiotic Resistance, and Human Epithelial Cell Invasion of Listeria monocytogenes
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 Title & Authors
Effect of NaCl on Thermal Resistance, Antibiotic Resistance, and Human Epithelial Cell Invasion of Listeria monocytogenes
Lee, Jin-Hee; Yoon, Hyun-Joo; Lee, Sun-Ah; Yoon, Yo-Han;
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 Abstract
This study evaluated the effects of NaCl on heat resistance and Caco-2 cell invasion of Listeria monocytogenes in broth media and sausage. A 10-strain mixture of L. monocytogenes was inoculated in tryptic soy broth containing 0.6% yeast extract (TSBYE), and sausage formulated with 0, 2, 4, and 6% NaCl. The medium was stored at 7, 15, 20, and for 3-16 d, and medium samples were withdrawn at the appropriate time and challenged to 55, 60, and to evaluate the thermal resistance of the pathogen. Sausage samples were stored at 7 and , and they were exposed to to evaluate thermal resistance. NaCl-habituated L. monocytogenes strains NCCP10811 and NCCP10943 were examined for 12 antibiotics and Caco-2 cell invasion assay (only L. monocytogenes NCCP10943). Bacterial populations of L. monocytogenes generally increased (p<0.05) during the heat challenge as NaCl concentrations increased in both TSBYE and sausage samples. The antibiotic resistance of L. monocytogenes was not observed () when it was exposed to a single concentration of NaCl in TSBYE, but the pathogen obtained resistance to some antibiotics when exposed to a sequential increase of NaCl concentration. Invasion efficiency of L. monocytogenes NCCP10943 was not increased () with NaCl concentration increase. These results indicate that NaCl may increase the resistance of L. monocytogenes to heat and to some antibiotics, but may not increase Caco-2 cell invasion of L. monocytogenes.
 Keywords
Listeria monocytogenes;NaCl;heat resistance;antibiotics;Caco-2 cell line;
 Language
English
 Cited by
1.
Effect of NaCl on Heat Resistance, Antibiotic Susceptibility, and Caco-2 Cell Invasion ofSalmonella, BioMed Research International, 2013, 2013, 1  crossref(new windwow)
2.
The Correlation between NaCl Adaptation and Heat Sensitivity of Listeria monocytogenes, a Foodborne Pathogen through Fresh and Processed Meat, Korean Journal for Food Science of Animal Resources, 2016, 36, 4, 469  crossref(new windwow)
3.
NaCl Influences Thermal Resistance and Cell Morphology ofEscherichia coliStrains, Journal of Food Safety, 2016, 36, 1, 62  crossref(new windwow)
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