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Development of Vitamin D Determination in Infant Formula by Column-Switching HPLC with UV Detector
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 Title & Authors
Development of Vitamin D Determination in Infant Formula by Column-Switching HPLC with UV Detector
Ko, Jin-Hyouk; Kwak, Byung-Man; Ahn, Jang-Hyuk; Shim, Sung-Lye; Kim, Kyong-Su; Yoon, Tae-Hyung; Leem, Dong-Gil; Jeong, Ja-Young;
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This study was carried out to develop an analytical method for the determination of vitamin D in infant formula. Vitamin D was determined by column-switching high-performance liquid chromatography (HPLC) equipped with a reversed phase column and UV detector after saponification and extraction of the formula with an organic solvent. A preseparation column (), focusing column (), analytical column () and UV-Vis detector (254 nm) were used. The limits of detection (LOD) and the limits of quantification (LOQ) for vitamin D were estimated to be and , respectively. The linearity, recovery, precision and accuracy of the analytical method for vitamin D were evaluated through the application of a SRM (Standard Reference Material) 1846 (National Institute of Standard & Technology, USA). The linearity of this method was calculated with a value of the coefficient of determination () . The recovery of vitamin D was . The intra-assay precision for vitamin D was between and , and the inter-assay precision for vitamin D ranged from to . The intra-assay accuracy for vitamin D was between and , and the inter-assay accuracy for vitamin D ranged from to . The proposed method is optimal for the separation and quantification of vitamin D from infant formula.
vitamin D;column-switching high-performance liquid chromatography;UV detection;infant formula;
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