Advanced SearchSearch Tips
Antioxidant, Antimicrobial, and Cytotoxic Activities of Ovotransferrin from Egg White
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Antioxidant, Antimicrobial, and Cytotoxic Activities of Ovotransferrin from Egg White
Moon, Sun-Hee; Lee, Jae-Hoon; Lee, Yong-Ju; Paik, Ji-Yeon; Ahn, Dong-Uk; Paik, Hyun-Dong;
  PDF(new window)
The antioxidant, antimicrobial, and cytotoxic activities of ovotransferrin were investigated in vitro. The antioxidant capacity of ovotransferrin was evaluated using the 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging method, antimicrobial effects using the agar well diffusion method, and cytotoxicity using the 3-(4,5-dimethylthizol-2-yl)-2,5-diphenylatetetrazolium bromide (MTT) assay. The DPPH radical-scavenging capacity of ovotransferrin at 1 mg/mL level reached approximately 60% after 48 h of reaction. The antimicrobial effects of ovotransferrin against common food-borne pathogens, Staphylococcus aureus KCCM 32395, Bacillus cereus KCCM 40935, Listeria monocytogenes ATCC 15313, Escherichia coli O157:H7 ATCC 43895, and Helicobacter pylori HpKCTC 26695 were dose dependant. Gram-positive bacteria was more sensitive to ovotransferrin than gram-negative bacteria. Ovotransferrin showed stronger antimicrobial effect against L. monocytogenes than other gram-positive bacteria tested. The cytotoxicity of ovotransferrin was evaluated in human cancer cell lines, various tissue origins, including the larynx (Hep-2), stomach (AGS), lung (SK-MES-1), liver (HepG2), breast (MCF-7), cervix (HeLa), and colon (HT-29). Ovotransferrin displayed relatively high cytotoxicity ( inhibition effects) at 40 mg/mL. At lower concentrations (), however, ovotransferrin cytotoxic effects were not significant in all cancer cell lines tested. These results indicated that ovotransferrin has potential to be used as an antioxidant or antimicrobial agent in foods or a pharmaceutical agent against cancers.
ovotransferrin;antioxidant activity;antimicrobial activity;cytotoxic activity;
 Cited by
Cytotoxic and antigenotoxic activities of phosvitin from egg yolk, Poultry Science, 2014, 93, 8, 2103  crossref(new windwow)
Bullen, J. J., Rogers, H. J., and Griffiths, E. (1978) Role of iron in bacterial infection. Curr. Top. Microbiol. Immunol. 80, 1-35. crossref(new window)

Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D., and Mitchell, J. B. (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res. 47, 936-942.

Davis, C. and Reeves, R. (2002) High value opportunities from the chicken egg. A report for the rural industries research and development corporation. Rural Ind. Res. Dev. Corp. Publ., Kingston, Australia.

Davalos, A., Miguel, M., Bartolome, B., and Lopez-Fandino, R. (2004) Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J. Food Prot. 67, 1939-1944.

Giansanti, F., Massucci, M. T., Giardi, M. F., Nozza, F., Pulsinelli, E., and Nicolini, C. (2005) Antiviral activity of ovotransferrin derived peptides. Biochem. Biophys. Res. Commun. 331, 69-73. crossref(new window)

Huang, W.-Y., Majumder, K., and Wu, J. (2010) Oxygen radical absorbance capacity of peptides from egg white protein ovotransferrin and their interaction with phytochemicals. Food Chem. 123, 635-641. crossref(new window)

Ibrahim, H. R. (1997) Isolation and characterization of the bactericidal domain of ovotransferrin. J. Agric. Chem. Soc. Japan 71, 39-41.

Ibrahim, H. R., Sugimoto, Y., and Aoki, T. (2000) Ovotransferrin antimicrobial peptide (OTAP-92) kills bacteria through a membrane damage mechanism. Biochim. Biophys. Acta 1523, 196-205. crossref(new window)

Ibrahim, H. R., Hoq, M. I., and Aoki, T. (2007) Ovotransferrin possesses SOD-like superoxide anion scavenging activity that is promoted by copper and manganese binding. Int. J. Biol. Macromol. 41, 631-640. crossref(new window)

Ibrahim, H. R. and Kiyono, T. (2009) Novel anticancer acitivity of the autocleaved ovotransferrin against human colon and breast cancer cells. J. Agric. Food Chem. 57, 11383- 11390. crossref(new window)

Jang, A. R., Lee, M. H., and Kim, J. C. (2005) Protein characteristics of ovotransferrin under the pH and temperature and its antimicrobial activity. J. Anim. Sci. Technol. 47, 1033-1040. crossref(new window)

Jee, H.-S., Chang, K.-H., Moon, S.-H., Park, S.-H., and Paik, H.-D. (2008) Anti-Helicobacter pylori, cytotoxic, and antiinflammatory activities of white ginseng extract. Food Sci. Biotechnol. 17, 1106-1109.

Kechaou, E. S., Dumay, J., Donnay-Moreno, C., Jaouen, P., Gouygou, J. P., Bergé, J. P., Amar, R. B. (2009) Enzymatic hydrolysis of cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) viscera using commercial proteases: effects on lipid distribution and amino acid composition. J. Biosci. Bioeng. 107, 158-164. crossref(new window)

Ko, K. Y. and Ahn, D. U. (2008) An economic and simple purification procedure for the large-scale production of ovotransferrin from egg white. Poultry Sci. 87, 1441-1450. crossref(new window)

Ko, K. Y., Mendonca, A. F., and Ahn, D. U. (2008a) Effect of ethylenediaminetetraacetate and lysozyme on the antimicrobial activity of ovotransferrin against Listeria monocytogenes. Poultry Sci. 87, 1649-1658. crossref(new window)

Ko, K. Y., Mendonca, A. F., and Ahn, D. U. (2008b) Influence of zinc, sodium bicarbonate, and citric acid on the antibacterial activity of ovotransferrin against Escherichia coli O157:H7 and Listeria monocytogenes in model systems and ham. Poultry Sci. 87, 2660-2670. crossref(new window)

Lee, K. A., Kim, K.-T., Nah, S.-Y., Chung, M.-S., Cho, S. W., and Paik, H.-D. (2011) Antimicrobial and antioxidative effects of onion peel extracted by the subcritical water. Food Sci. Biotechnol. 20, 543-548. crossref(new window)

Li-Chan, E. and Nakai, S. (1989) Biochemical basis for the properties of egg white. Crit. Rev. Poult. Biol. 2, 21-58.

Mine, Y. (2007) Egg proteins and peptides in human healthchemistry, bioactivity and production. Curr. Pharm. Des. 13, 875-884. crossref(new window)

Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxixity assay. J. Immunol. Methods 65, 55-63. crossref(new window)

Nakamura, S. and Kato, A. (2000) Multi-functional biopolymer prepared by covalent attachment of galactomannan to egg-white proteins through naturally occurring Maillard reaction. Nahrung. 44, 201-206. crossref(new window)

Oguro, T., Ohaki, Y., Asano, G., Ebina, T., and Watanabe, K. (2001) Ultrastructural and immunohistochemical characterization on the effect of ovomucin in tumor angiogenesis. Jpn. J. Clin. Electron Microsc. 33, 89-99.

Park, W. B., Choung, B. Y., Park, S. M., Kim, H. S., and Lyu, S. Y. (1999) Effects of drying process of mistletoes on cytotoxicities against cultured HL-60 and Molt-4 cells. Food Sci. Biotechnol. 8, 391-396.

Rath, N. C., Anthony, N. B., Kannan, L., Huff, W. E., Huff, G. R., and Chapman, H. D. (2009) Serum ovotransferrin as a biomarker of inflammatory diseases in chickens. Poultry Sci. 88, 2069-2074. crossref(new window)

Schade, A. L. and Caroline, L. (1944) Raw hen egg white and the role of iron in growth inhibition of Shigella dysenteriae, Staphylococcus aureus, Escherichia coli and Saccharomyces cerevisiae. Science 100, 14-15. crossref(new window)

Shen, S., Chahal, B., Majumder, K., You, S.-J., and Wu, J. (2010) Identification of novel antioxidative peptides derived from a thermolytic hydrolysate of ovotransferrin by LC-MS/ MS. J. Agric. Food Chem. 58, 7664-7672. crossref(new window)

Taye, B., Giday, M., Animut, A., and Seid, J. (2011) Antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia. Asian Pac. J. Trop. Biomed. 370-375.

Valenti, P., Visca, P., Antonini, G., and Orsi, N. (1985) Antifungal activity of ovotransferrin towards genus Candida. Mycopathologia 89, 169-175. crossref(new window)

Voidarou, C., Alexopoulos, A., Plessas, S., Karapanou, A., Mantzourani, I., Stavropoulou, E., Fotou, K., Tzora, A., Skoufos, I., and Bezirtzoglou, E. (2011) Antibacterial activity of different honeys against pathogenic bacteria. Anaerobe 17, 375-379. crossref(new window)

Watanabe, K., Tsuge, Y., Shimoyamada, M., Ogama, N., and Ebina, T. (1998) Antitumor effects of Pronase-treated fragments, glycopeptides, from ovomucin in hen egg white in a double grafted tumor system. J. Agric. Food Chem. 46, 3033-3038. crossref(new window)

Williams, J. (1968) A comparison of glycopeptides from the ovotransferrin and serum transferrin of the hen. Biochem. J. 108, 57-67.

Wu, J. P. and Alexandra, A. L. (2011) Ovotransferrin: Structure, bioactivities, and preparation. Food Res. Int. 46, 480-487.

Xie, H., Newberry, L., Clark, F. D., Huff, W. E., Huff, G. R., and Balog, J. M. (2002) Changes in serum ovotransferrin levels in chickens with experimentally induced inflammation and diseases. Avian Dis. 46, 122-131. crossref(new window)

Xu, X., Shigeru, K., and Mine, Y. (2007) Antioxidant activity of tryptic digests of hen egg yolk phosvitin. J. Sci. Food Agric. 87, 2604-2608. crossref(new window)

Yamamoto, T. (1997) Hen Eggs: Their Basic and Applied Science. In: Insights into the structure-function relationship of ovalbumin, ovotransferrin and lysozyme. CRC Press, Boca Raton, FL, 37-59.

Yi, H. J., Kim, J. Y., Kim, K. H., Lee, H. J., and Lee, H. J. (2003) Anticancer activity of peptide fractions from egg with hydrolysate against mouse lymphoma cells. Food Sci. Biotechonol. 12, 224-227.