Advanced SearchSearch Tips
Prevalence and Antimicrobial Resistance of Enterococus faecalis and Enterococcus faecium Isolated from Beef, Pork, Chicken and Sashimi
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Prevalence and Antimicrobial Resistance of Enterococus faecalis and Enterococcus faecium Isolated from Beef, Pork, Chicken and Sashimi
Sung, Chang-Hyun; Chon, Jung-Whan; Kwak, Hyo-Sun; Kim, Hyunsook; Seo, Kun-Ho;
  PDF(new window)
In this study, a total of 256 samples of retail raw meats (beef, pork and chicken) and sashimi were investigated for the presence of Enterococcus faecalis and Enterococcus faecium. We isolated a total of 117 E. faecalis and E. faecium from the samples, with contamination rates ranging from 18.8% for sashimi samples to 68.8% of chicken samples. E. faecalis was the predominant species recovered from all of the retail raw meats beef (42.2%), pork (42.2%), chicken (65.6%) and sashimi (12.5%). Among 117 isolates, 61 isolates (52.1%) were resistant to tetracycline, 32 isolates (27.4%) were resistant to erythromycin, 23 isolates (19.7%) were resistant to chloramphenicol, 16 isolates (13.7%) were resistant to ripampin, 10 isolates (8.5%) were resistant to gentamycin, 9 isolates (7.7%) were resistant to ciprofloxacin and 1 isolate (0.9%) was resistant to ampicillin and penicillin G. No resistance to amoxicillin + clavulanic acid and vancomycin was observed. Although no strain was resistant to vancomycin, the vanB gene was observed in 9 of 117 of Enterococcus (7.7%) demonstrating potential risk of vancomycin-resistant Enterococcus (VRE). Our results indicate that E. faecalis and E. faecium were highly prevalent in retail raw meats, but most strains were sensitive to tested antibiotics.
Enterococcus;prevalence;antimicrobial resistance;vancomycin-resistant Enterococcus;
 Cited by
축산물과 수산물에서 분리된 장구균의 항생제 감수성 및 유전형 분석,김윤정;오미현;김용훈;김순한;박건상;주인선;

한국식품위생안전성학회지, 2014. vol.29. 3, pp.228-233 crossref(new window)
Monitoring of Antimicrobial Resistance and Genetic Analysis of Enterococcus spp. Isolated from Beef, Pork, Chicken and Fish in Korea, Journal of Food Hygiene and Safety, 2014, 29, 3, 228  crossref(new windwow)
Cauwerts, K., Decostere, A., De Graef, E. M., Haesebrouck, F., and Pasmans, F. (2007) High prevalence of tetracycline resistance in Enterococcus isolates from broilers carrying the erm(B) gene. Avian Pathol. 36, 395-399. crossref(new window)

Cha, C. H., An, H. K., and Kim, J. U. (2010) Detection of vancomycin-resistant enterococci using multiplex real-time PCR assay and melting curve analysis. Korean J. Lab. Med. 30, 138-146. crossref(new window)

Chadwick, P. R., Woodford, N., Kaczmarski, B., Gray, S., Barrell, R. A., and Oppenheim, B. A. (1996) Glycopeptide resistant enterococci isolated from uncooked meat. J. Antimicrob. Chemother. 38, 908-909. crossref(new window)

De Fatima Silva Lopes, M., Ribeiro, T., Abrantes, M., Figueiredo Marques, J. J., Tenreiro, R., and Crespo, M. T. (2005) Antimicrobial resistance profiles of dairy and clinical isolates and type strains of enterococci. Int. J. Food Microbiol. 103, 191-198. crossref(new window)

Franz, C. M., Holzapfel, W. H., and Stiles, M.E. (1999) enterococci at the crossroads of food safety. Int. J. Food Microbiol. 47, 1-24. crossref(new window)

Ham, H. J. (2007) E. faecalis and E. faecium isolated in dried marine products. J. Fd. Hyg. Safety 22, 294-299.

Harwood, V. J., Brownell, M., Perusek, W., and Whitlock, J. E. (2001) Vancomycin-resistant Enterococcus spp. isolated from wastewater and chicken feces in the United States. Appl. Environ. Microbiol. 67, 4930-4933. crossref(new window)

Kang, T. M., Cho, S. K., and Park, J. H. (2008) Antibiotic resistances of enterococcus isolated from salad and sprout. Kor. J. Microbiol. Biotechnol. 36, 142-148.

Katie, F. and Carol, P. (2009) The ecology, epidemiology and virulence of Enterococcus. Microbiology 155, 1749-1757. crossref(new window)

Kim, A., Cho, M. I., Her, M., Jung, B. Y., Lim, S. K., Jung, S. C., Song, C. H., and Lee, J. Y. (2011) Quinupristin/dalfopristin resistance patterns in Enterococcus faecium isolated from chicken farms in South Korea. Kor. J. Vet. Publ. Hlth. 35, 91-96.

Kim, S. H., Kim, J. S., and Park, J. H. (2007) Antibiotic resistance of Enterococcus isolated from the processed grain foods, saengsik and sunsik. Food Sci. Biotechnol. 16, 470-476.

Klein, G., Pack, A., and Reuter, G. (1998) Antibiotic resistance patterns of enterococci and occurrence of vancomycin-resistant enterococci in raw minced beef and pork in Germany. Appl. Environ. Microbiol. 64, 1825-1830.

Kummerer, K. (2003) Significance of antibiotics in the environment. J. Antimicrob. Chemother. 52, 5-7. crossref(new window)

Lee, H. I., Lee, S. J., and Choi, S. S. (2009) Antimicrobial resistance patterns of enterococci spp. isolated from raw milk samples. J. Fd. Hyg. Safety 24, 373-377.

Mandel, G. L., Bennett, J. E., Dolin, R., and Mandell, D. (1995) Streptococcus bovis, and Leuconostoc spp. In: A principles and practice of infectious disease. Mcellering, R. C. (ed) Churchill Living Stone, NY, pp. 1826-1835.

NCCLS (National Committee for Clinical Laboratory Standards) (2004) Performance standards for antimicrobial susceptibility testing. NCCLS document M100-S14. National Committee for Clinical Laboratoy Standards. Wayne, PA., p.65.

Noble, W. C., Virani, Z., and Cree, R. G. (1992) Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS. Microbiol. Lett. 93, 195-198. crossref(new window)

Oh, E. G., Son, K. T., Yu, H. S., Kim, J. H., Lee, T. S., and Lee, H. J. (2008) Antimicrobial susceptibility pattern of Enterococcus faecalis and E. faecium from fish farms in the southern coast of Korea. J. Kor. Fish Soc. 41, 435-439. crossref(new window)

Park, H. S., Chung, H. K., and Lee, H. H. (1992) Antimicrobial susceptibility of Enterococcus species isolated from clinical materials. J. Korean Soc. Microbiol. 27, 103-114.

Park, I. J., Lee, W. G., Lee, H., Yong, D., Lee, K., Kim, E. C., Jeong, S. H., Park, Y. J., Choi, T. Y., Uh, Y., Shin, J. H., Lee, J., Ahn, J. Y., Lee, S. H., and Woo, G. J. (2006) Mechanism of vanB phenotype in vancomycin-resistant enterococci carrying vanA gene. Korean J. Lab. Med. 26, 412-417. crossref(new window)

Pavia, M., Nobile, C. G., Salpietro, L., and Angelillo, I. F. (2000) Vancomycin resistance and antibiotic susceptibility of enterococci in raw meat. J. Food Prot. 63, 912-915. crossref(new window)

Quednau, M., Ahrn, S., Petersson, A. C., and Molin, G. (1998) Antibiotic-resistant strains of Enterococcus isolated from Swedish and Danish retailed chicken and pork. J. Appl. Microbiol. 84, 1163-1170. crossref(new window)

Schlefer, K. H. and Kilpper-Balz, R. (1987) Molecular and chemotaxonomic approach to the classification of streptococci, enterococci and lactococci. Rev. Syst. Appl. Microbiol. 10, 1-18. crossref(new window)

Sood, S., Malhotra, M., Das, B. K., and Kapil, A. (2008) Enterococcal infections and antimicrobial resistance. Indian J. Med. Res. 128, 111-121.

Sung, C. H., Cheon, J. H., Hyeon, J. Y., Hwang, I. G., Kwak, H. S., Yoon, S. H., Lee, J. S., Chung, Y. H., Song, K. Y., and Seo, K. H. (2010) Prevalence and antimicrobial resistance of Vibrio parahaemolyticus isolated from raw fishes. Kor. J. Vet. Publ. Hlth. 34, 197-202.

Tailer, S. A. N., Bailey, E. M., and Rybak, M. J. (1993) Enterococcus, an emerging pathogen. Ann. Pharmacother. 27, 1231-1242. crossref(new window)