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Anti-oxidative and Neuroprotective Activities of Pig Skin Gelatin Hydrolysates
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 Title & Authors
Anti-oxidative and Neuroprotective Activities of Pig Skin Gelatin Hydrolysates
Kim, Dong Wook; Park, Kimoon; Ha, Goeun; Jung, Ju Ri; Chang, Ounki; Ham, Jun-Sang; Jeong, Seok-Geun; Park, Beom-Young; Song, Jin; Jang, Aera;
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 Abstract
This study was conducted to determine the antioxidative and neuroprotective effect of pig skin extracts (PS) and pig skin gelatin hydrolysates (LPS) using a human neuroblastoma cell line (SH-SY5Y). The extraction yield of PS was 3 fold higher than that of LPS. The protein content of PS was about 10 fold higher than that of LPS (p<0.05). Also LPS increased antioxidative activity dose dependently, and the activity was significantly higher than PS at all concentration (p<0.05). DPPH radical scavenging activity of LPS at 50 mg/mL was 92.97%, which was similar to vitamin C as a positive control. ABTS radical scavenging activity of LPS (20 mg/mL) was 89.83% and oxygen radical absorbance capacity of LPS at 1 mg/mL was Trolox Equvalent/g. No significant change of human neuroblastoma cells was determined by MTT test. Cell death by oxidative stress induced by and amyloid beta 1-42 () was protected by LPS rather than PS. Acetylcholine esterase was significantly inhibited, by up to 33.62% by LPS at 10 mg/mL. Therefore, these results suggest that pig skin gelatin hydrolysates below 3 kDa have potential to be used as anti-oxidative and neuroprotective functional additives in the food industry, while further animal test should be determined in the future.
 Keywords
gelatin hydrolysates;collagen;neuroblastoma cell;acetylcholine esterase;pig skin;
 Language
Korean
 Cited by
1.
제주 재래마와 더러브렛종과의 교잡종인 한라마 사골추출물과 효소분해물의 항산화 효과,김동욱;박재인;채현석;김영붕;장애라;

생명과학회지, 2013. vol.23. 9, pp.1147-1154 crossref(new window)
2.
Antioxidant Activity of Porcine Skin Gelatin Hydrolyzed by Pepsin and Pancreatin,;;;;;;;;;;

한국축산식품학회지, 2013. vol.33. 4, pp.493-500 crossref(new window)
3.
당나귀 사골과 껍질의 항산화기능 및 SK-N-SH세포 보호효과,김동욱;채현석;김남영;장애라;

생명과학회지, 2013. vol.23. 8, pp.1019-1024 crossref(new window)
4.
Comparison of Antioxidant Activities of Hydrolysates of Domestic and Imported Skim Milk Powders Treated with Papain,;;;;;;

한국축산식품학회지, 2015. vol.35. 3, pp.360-369 crossref(new window)
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Antioxidant Activity of Porcine Skin Gelatin Hydrolyzed by Pepsin and Pancreatin, Korean Journal for Food Science of Animal Resources, 2013, 33, 4, 493  crossref(new windwow)
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