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Anti-oxidative and Neuroprotective Activities of Pig Skin Gelatin Hydrolysates
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 Title & Authors
Anti-oxidative and Neuroprotective Activities of Pig Skin Gelatin Hydrolysates
Kim, Dong Wook; Park, Kimoon; Ha, Goeun; Jung, Ju Ri; Chang, Ounki; Ham, Jun-Sang; Jeong, Seok-Geun; Park, Beom-Young; Song, Jin; Jang, Aera;
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This study was conducted to determine the antioxidative and neuroprotective effect of pig skin extracts (PS) and pig skin gelatin hydrolysates (LPS) using a human neuroblastoma cell line (SH-SY5Y). The extraction yield of PS was 3 fold higher than that of LPS. The protein content of PS was about 10 fold higher than that of LPS (p<0.05). Also LPS increased antioxidative activity dose dependently, and the activity was significantly higher than PS at all concentration (p<0.05). DPPH radical scavenging activity of LPS at 50 mg/mL was 92.97%, which was similar to vitamin C as a positive control. ABTS radical scavenging activity of LPS (20 mg/mL) was 89.83% and oxygen radical absorbance capacity of LPS at 1 mg/mL was Trolox Equvalent/g. No significant change of human neuroblastoma cells was determined by MTT test. Cell death by oxidative stress induced by and amyloid beta 1-42 () was protected by LPS rather than PS. Acetylcholine esterase was significantly inhibited, by up to 33.62% by LPS at 10 mg/mL. Therefore, these results suggest that pig skin gelatin hydrolysates below 3 kDa have potential to be used as anti-oxidative and neuroprotective functional additives in the food industry, while further animal test should be determined in the future.
gelatin hydrolysates;collagen;neuroblastoma cell;acetylcholine esterase;pig skin;
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