JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Proteomic Analysis and Protective Effects of Outer Membrane Proteins from Salmonella Gallinarum in Chickens
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Proteomic Analysis and Protective Effects of Outer Membrane Proteins from Salmonella Gallinarum in Chickens
Sun, Jisun; Cho, Youngjae; Jang, Joo-Hyun; Kang, Zheng-Wu; Han, Jang-Hyuk; Hahn, Tae-Wook;
  PDF(new window)
 Abstract
Salmonella Gallinarum (SG) is known as an important pathogen that causes fowl typhoid in chickens. To investigate SG outer-membrane proteins (OMPs) as a vaccine candidate, we used proteomic mapping and database analysis techniques with extracted OMPs. Also, extracted OMPs were evaluated in several aspects to their safety, immune response in their host and protective effects. Our research has established a proteomic map and database of immunogenic SG-OMPs used as inactive vaccine against salmonellosis in chickens. A total of 22 spots were detected by 2-dimensional gel electrophoresis and immunogenic protein analysis. Eight spots were identified by Matrix-Assisted Laser Desorption/Ionization-Time of Flight-Mass spectrometry (MALDI-TOF-MS) and peptide mass fingerprinting (PMF) and categorized into four different types of proteins. Among these proteins, OmpA is considered to be an immunogenic protein and involved in the hosts' immune system. To estimate the minimum safety dose in chickens, 35 brown layers were immunized with various concentrations of OMPs, respectively. Consequently, all chickens immunized with more than a dose were protected against challenges. Moreover, intramuscular administration of OMPs to chickens was more effective compared to subcutaneous administration. These results suggest that the adjuvanted SG-OMP vaccine not only induces both the humoral and cellular immune response in the host but also highly protects the hosts' exposed to virulent SG with OMPs extracted by our method.
 Keywords
Salmonella Gallinarum;outer membrane proteins (OMPs);proteomic analysis;protective effects;OMP vaccine;
 Language
Korean
 Cited by
 References
1.
Babu, U., Scott, M., Myers, M. J., Okamura, M., Gaines, D., Yancy, H. F., Lillehoj, H., Heckert, R. A., and Raybourne, R. B. (2003) Effects of live attenuated and killed Salmonella vaccine on T-lymphocyte mediated immunity in laying hens. Vet. Immunol. Immunopathol. 10, 39-44.

2.
Barrow, P. A., Huggins, M. B., and Lovell, M. A. (1994) Host specificity of Salmonella infection in chickens and mice is expressed in vivo primarily at the level of the reticuloendothelial system. Infect. Immun. 62, 4602-4610.

3.
Bouzoubaa, K., Nagaraja, K. V., Newman, J. A., and Pomeroy, B. S. (1987) Use of membrane proteins from Salmonella Gallinarum for prevention of fowl typhoid infection in chickens. Avian Dis. 31, 699-704. crossref(new window)

4.
Clarke, R. C. and Gyles, C. L. (1993) Salmonella. In: Pathogenesis of Bacterial Infections in Animal. Gyles, C. L., and Thoen, C. O. (eds). 2nd ed, Iowa State University Press, Ames, IA, pp. 199-153.

5.
Collins, F. M. and Campbell, S. G. (1982) Immunity to intracellular bacteria. Vet. Immunol. Immunopathol. 3, 5-66. crossref(new window)

6.
Cordwell, S. J. (2006) Technologies for bacterial surface proteomics. Curr. Opin. Microbiol. 9, 320-329. crossref(new window)

7.
Eswarappa, S. M., Janice, J., Balasundaram, S. V., Dixit, N. M., and Chakravortty, D. (2009) Host-specificity of Salmonella enterica serovar Gallinarum: Insights from comparative genomics. Infect. Genet. Evol. 9, 468-473. crossref(new window)

8.
Ewing, W. H. (1986) Edwards and Ewing's Identification of Enterobacteriaceae. 4th ed, Elsevier Science Publishing, NY, pp. 247-318.

9.
Guard-Petter, J. (2001) The chicken, the egg and Salmonella Enteritidis. Environ. Microbiol. 3, 421-430. crossref(new window)

10.
Lee, H. S., Lim, S. K., Cho, Y. S., Joo, Y. S., Kim, J.H., and Kim, J. M. (2007) Protective effects of mix-crude outer membrane protein Salmonella vaccine against salmonellosis in chickens and pigs. Korean J. Vet. Res. 42, 147-155.

11.
Lee, J. S., Jung, I. D., Lee, C. M., Park, J. W., Chun, S. H., Jeong, S. K., Ha, T. K., Shin, Y. K., Kim, D. J., and Park, Y. M. (2010) Outer membrane protein a of Salmonella enterica serovar Typhimurium activates dendritic cells and enhances Th1 polarization. BMC Microbiol. 15, 263-270.

12.
Lillehoj, H. S. (1991) Cell-mediated immunity in parasitic and bacterial diseases. In: Avian cellular immunology. Sharma, J. M. (ed) CRC Press, Boca Raton, FL, pp. 155-181.

13.
Meenakshi, M., Bakshi, C. S., Butchaiah, G., Bansal, M. P., Siddiqui, M. Z., Singh, V. P. (1999) Adjuvanted outer membrane protein vaccine protects poultry against infection with Salmonella Enteritidis. Vet. Res. Commun. 23, 81-90. crossref(new window)

14.
Popoff, M. Y., Bockemuhl, J., and Gheesling, L. L. (2004) Supplement 2002 (no. 46) to the Kauffmann-White scheme. Res. Microbiol. 155, 568-570. crossref(new window)

15.
Rabilloud, T., Kieffer, S., Procaccio, V., Louwagie, M., Courchesne, P. L., Patterson, S. D., Martinez, P., Garin, J., and Lunardi, J. (1998) Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: Toward a human mitochondrial proteome. Electrophoresis 19, 1006-1014. crossref(new window)

16.
Seo, Y. S., Lee, S. H., Shin, E. K., Kim, S. J., Jung, R., Hahn, T. W. (2006) Pulsed-field gel electrophoresis genotyping of Salmonella Gallinarum and comparison with random amplified polymorphic DNA. Vet. Microbiol. 115, 349-357. crossref(new window)

17.
Silva, E. N., Snoeyenbos, G. H., Weinack, O. M., and Smyser, C. F. (1981) Studies on the use of 9R strain of Salmonella Gallinarum as a vaccine in chickens. Avian Dis. 25, 38-52. crossref(new window)

18.
Smith, I. M. (1969) Protection against experimental fowl typhoid by vaccination with strain 9R reconstituted from the freeze-dried state. J. Comp. Pathol. 79, 197-205. crossref(new window)

19.
Valiente Moro, C., De Luna, C. J., Tod, A., Guy, J. H., Sparagano, O. A., and Zenner, L. (2009) The poultry red mite (Dermanyssus gallinae): A potential vector of pathogenic agents. Exp. Appl. Acarol. 48, 93-104. crossref(new window)

20.
Wigley, P., Hulme, S., Powers, C., Beal, R., Smith, A., and Barrow, P. (2005) Oral infection with the Salmonella enterica serovar Gallinarum 9R attenuated live vaccine as a model to characterize immunity to fowl typhoid in the chicken. BMC Vet. Res. 12, 2-7.

21.
Wu, M., Stockley, P. G., and Martin, W. J. 2nd. (2002) An improved western blotting technique effectively reduces background. Electrophoresis 23, 2373-2376. crossref(new window)