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Antioxidant Activity of Porcine Skin Gelatin Hydrolyzed by Pepsin and Pancreatin
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 Title & Authors
Antioxidant Activity of Porcine Skin Gelatin Hydrolyzed by Pepsin and Pancreatin
Chang, Oun Ki; Ha, Go Eun; Jeong, Seok-Geun; Seol, Kuk-Hwan; Oh, Mi-Hwa; Kim, Dong Wook; Jang, Aera; Kim, Sae Hun; Park, Beom-Young; Ham, Jun-Sang;
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Gelatin is a collagen-containing thermohydrolytic substance commonly incorporated in cosmetic and pharmaceutical products. This study investigated the antioxidant activity of gelatin by using different reagents, such as 2,2-azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), 2,2-di (4-tert-octylphenyl)-1-picrylhydrazyl (DPPH), and oxygen radical absorbance capacity-fluorescein (ORAC-FL) in a porcine gelatin hydrolysate obtained using gastrointestinal enzymes. Electrophoretic analysis of the gelatin hydrolysis products showed extensive degradation by pepsin and pancreatin, resulting in an increase in the peptide concentration (12.1 mg/mL). Antioxidant activity, as measured by ABTS, exhibited the highest values after 48-h incubation with pancreatin treatment after pepsin digestion. Similar effects were observed at 48 h incubation, that is, 61.5% for the DPPH assay and 69.3% for the ABTS assay. However, the gallic acid equivalent (GE) at 48 h was , whereas GE was obtained using the ABTS and DPPH assays, indicating about sixfold increase. In the ORACFL assay, antioxidant activity corresponding to of trolox equivalent was found in the gelatin hydrolysate after 24 h hydrolysis with pancreatin treatment after pepsin digestion, whereas this activity decreased at 48 h. These antioxidant assay results showed that digestion of gelatin by gastrointestinal enzymes prevents oxidative damage.
antioxidant activity;gelatin;pepsin;pancreatin;hydrolysis;
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Ao, J. and Li, B. (2012) Amino acid composition and antioxidant activities of hydrolysates and peptide fractions from porcine collagen. Food Sci. Technol. Int. 18, 425-434. crossref(new window)

Becker, G. L. (1993) Preserving food and health: Antioxidants make functional, nutritious preservatives. Food Processing 12, 54-56.

Calabrese, V., Lodi, R., Tonon, C., D'Agata, V., Sapienza, M., Scapagnini, G., Mangiameli, A., Pennisi, G., Giuffrida Stella, A. M., and Butterfield, D. A. (2005) Oxidative stress, mitochondrial dysfunction and cellular stress response in Friedrich's ataxia. J. Neurological Sci. 233, 145-162. crossref(new window)

Chang, O. K., Humbert, G., Gaillard, J. L., and Lee, B. O. (2006) Characterization of equine milk and cheese making. Korean J. Food Sci. An. 26, 368-374.

Chang, O. K., Perrin, C., Galia, W., Saulnier, F., Miclo, L., Roux, E., Driou, A., Humbert, G., and Dary, A. (2012) Release of the cell-envelope protease PrtS in the growth medium of Streptococcus thermophilus 4F44. Int. Dairy J. 23, 91-98. crossref(new window)

Chang, O. K., Ha, G. E., Han, K. S., Seol, K. H., Kim, H. W., Jeong, S. G., Oh, M. H., Park, B. Y., and Ham, H. S. (2013a). Novel antioxidant peptide derived from the ultrafiltrate of ovomucin hydrolysate. J. Agri. Food Chem. 61, 7294-7300. crossref(new window)

Chang, O. K., Seol, K. H., Jeong, S. G., Oh, M. H., Park, B. Y., Perrin, C., and Ham, J. S. (2013b) Casein hydrolysis by Bifidobacterium longum KACC91563 and antioxidant activities of peptides derived therefrom. J. Dairy. Sci. DOI: 10. 3168/jds.2013-6687 crossref(new window)

Cho, Y. (2009) Comparative study of lactic acid bacteria for antioxidative and casein phosphopeptide-producing activities. Graduate School, Chonnam National University, Korea.

Church, F. C., Swaisgood, H. E., Porter, D. H., and Catignani, G. L. (1983) Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J. Dairy Sci. 66, 1219-1227. crossref(new window)

Cole, C. G. B. (2000) Gelatin. In: Encyclopedia of Food Science and Technology. Francis, F. J. (ed) John Wiley & Sons, New York, pp. 1183-1188.

Davalos, A., Miguel, M., Bartolome, B., and Lopez-Fandino, R. (2004) Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J. Food Protect. 67, 1939-1944.

de Mejia, E. and de Lumen, B. O. (2006) Soybean bioactive peptides: A new horizon in preventing chronic diseases. Sexuality, Reproduction & Menopause 4, 91-95. crossref(new window)

Frister, H., Meisel, H., and Schlimme, E. (1988) OPA method modified by use of N,N-dimethyl-2-mercaptoethylammonium chloride as thiol component. Fresenius' J. Anal. Chem. 330, 631-633. crossref(new window)

Gimenez, B., Aleman, A., Montero, P., and Gomez-Guillen, M. C. (2009) Antioxidant and functional properties of gelatin hydrolysates obtained from skin of sole and squid. Food Chem. 114, 976-983. crossref(new window)

Gomez-Guillen, M. C., Gimenez, B., Lopez-Caballero, M. E., and Montero. M. P. (2011) Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloid. 25, 1813-1827. crossref(new window)

Hernandez-Ledesma, B., Davalos, A., Bartolome, B., and Amigo, L. (2005) Preparation of antioxidant enzymatic hydrolysates from $\alpha$-lactalbumin and $\beta$-lactoglobulin. identification of active peptides by HPLC-MS/MS. J. Agric. Food Chem. 53, 588-593. crossref(new window)

Je, J. Y., Park, P. J., and Kim, S. K. (2004). Free radical scavenging properties of hetero-chitooligosaccharides using an ESR spectroscopy. Food Chem. Toxicol.42, 381-387. crossref(new window)

Kim, D. W., Park, K., Ha, G. E., Jung, J. R., Chang, O. K., Ham, J. S., Jeong, S. G., Park, B. Y., Song, J., and Jang, A. (2013) Anti-oxidative and neuroprotective activities of pig skin gelatin hydrolysates. Korean J. Food Sci. An. 33, 258-267. crossref(new window)

Kim, S., Kim, Y., Byun, H., Nam, K., Joo, D., and Shahidi, F. (2001) Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska Pollack skin. J. Agric. Food Chem. 49, 1984-1989. crossref(new window)

Korhonen, H. (2009) Milk-derived bioactive peptides: From science to applications. J. Funct. Foods 1, 177-187. crossref(new window)

Kullisaar, T., Songisepp, E., Mikelsaar, M., Zilmer, K., Vihalemm, T., and Zilmer, M. (2003) Antioxidative probiotic fermented goats' milk decreases oxidative stress-mediated atherogenicity in human subjects. British J. Nutr. 90, 449-456. crossref(new window)

Li, B., Chen, F., Wang, X., Ji, B., and Wu, Y. (2007) Isolation and identification of antioxidative peptides from porcine collagen hydrolysate by consecutive chromatography and electrospray ionization-mass spectrometry. Food Chem. 102, 1135-1143. crossref(new window)

Lin, L. and Li, B. (2006) Radical scavenging properties of protein hydrolysates from Jumbo flying squid (Dosidicus eschrichitii Steenstrup) skin gelatin. J. Sci. Food Agric. 86, 2290-2295. crossref(new window)

Madhujith, T., Izydorczyk, M., and Shahidi, F. (2006) Antioxidant properties of pearled barley fractions. J. Agric. Food Chem. 54, 3283-3289. crossref(new window)

Mark, S. and Alger, M. (1997) Polymer science dictionary. Springer. p. 152.

Mendis, E., Rajapakse, N., Byun, H., and Kim, S. (2005) Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects. Life Sci. 77, 2166-2178. crossref(new window)

Miguel, M., Contreras, M. M., Recio, I., and Aleixandre, A. (2009) ACE-inhibitory and antihypertensive properties of a bovine casein hydrolysate. Food Chem. 112, 211-214. crossref(new window)

Minervini, F., Algaron, F., Rizzello, C. G., Fox, P. F., Monnet, V., and Gobbetti, M. (2003) Angiotensin I-converting-enzymeinhibitory and antibacterial peptides from Lactobacillus helveticus PR4 proteinase-hydrolyzed caseins of milk from six species. Appl. Environ. Microbiol. 69, 5297-5305. crossref(new window)

Nimalaratne, C., Lopes-Lutz, D., Schieber, A., and Wu, J. (2011) Free aromatic amino acids in egg yolk show antioxidant properties. Food Chem. 129, 155-161. crossref(new window)

Oboh, G., Adefegha, S. A., Ademosun, A. O., and Unu, D. (2010) Effects of hot water treatment on the phenolic photochemicals and antioxidant activities of lemon grass (Cymbopogon itratus). Electron. J. Environ. Agric. Food Chem. 9, 503-513.

Ou, B., Hampsch-Woodill, M., and Prior, R. L. (2001) Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J. Agric. Food Chem. 49, 4619-4626. crossref(new window)

Prior, R. L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B., and Jacob, R. (2003) Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORACFL)) of plasma and other biological and food samples. J. Agric. Food Chem. 51, 3273-3279. crossref(new window)

Que, F., Mao, L., Zhu, C., and Xie, G. (2006) Antioxidant properties of Chinese yellow wine, its concentrate and volatiles. LWT-Food Sci. Technol. 39, 111-117. crossref(new window)

Rajapakse, N., Mendis, E., Jung, W. K., Je, J. Y., and Kim, S. K. (2005) Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties. Food Res. Int. 38, 175-182. crossref(new window)

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol.Med. 26, 1231-1237. crossref(new window)

Ren, J., Zhao, M., Shi, J., Wang, J., Jiang, Y., Cui, C., Kakuda, Y., and Xue, S. J. (2008) Purification and identification of antioxidant peptides from grass carp muscle hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry. Food Chem. 108, 727-736. crossref(new window)

Rho, S. J., Lee, J. S., Chung, Y. I., Kim, Y. W., and Lee, H. G. (2009) Purification and identification of an angiotensin I-converting enzyme inhibitory peptide from fermented soybean extract. Proc. Biochem. 44, 490-493. crossref(new window)

Samaraweera, H., Zhang, W. G., Lee E. J., and Ahn, D. U. (2011) Egg yolk phosvitin and functional phosphopeptidesreview. J. Food Sci. 76, 143-150.

Sarmadi, B. H. and Ismail, A. (2010) Antioxidative peptides from food proteins: A review. Peptides. 31, 1949-1956. crossref(new window)

Singh, S., Rao, K. V. Rama., Venugopal, K., and Manikandan, R. (2002) Alteration in dissolution characteristics of gelatincontaining formulations: A review of the problem, test methods, and solutions. Pharmaceutical Technol. 26, 36-58.

Wellman-Labadie, O., Picman, J., and Hincke, M. T. (2008) Comparative antibacterial activity of avian egg white protein extracts. Br. Poult. Sci. 49, 125-132. crossref(new window)

Yang, J., Ho, H., Chu, Y., and Chow, C. (2008) Characteristic and antioxidant activity of retorted gelatin hydrolysates from cobia (Rachycentron canadum) skin. Food Chem. 110, 128-136. crossref(new window)