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Rapid and Sensitive Detection of Salmonella spp. by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample
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 Title & Authors
Rapid and Sensitive Detection of Salmonella spp. by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample
Cho, Ae-Ri; Dong, Hee-Jin; Cho, Seongbeom;
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 Abstract
In this study, a rapid and sensitive detection tool for screening Salmonella spp. by using a loop-mediated isothermal amplification (LAMP) assay targeting the genomic sequence of the invA gene was developed. The inclusivity and exclusivity were both at 100% in the analysis, and the limit of detection (LOD) in a pure S. Enteritidis culture suspended in saline was CFU/mL at 18.17 min ( = 0.9446). The LODs of the LAMP assay in buffered peptone water with Salmonella (BPW) and duck carcass swab sample enriched in BPW with Salmonella (BPWS) after 0 and 12 h incubation were CFU/mL and CFU/mL, respectively. Comparing the LODs in BPW with those in BPWS, the LAMP assay was less influenced by compounds of duck carcass swab sample than the PCR assay. Sensitivity and specificity of the LAMP assay in 50 duck carcass swab samples enriched in BPW for 6 h were 96% and 84%, respectively, indicating that the LAMP assay is a rapid, simple and sensitive assay, which can be utilized as a potential screening tool of Salmonella spp. in duck carcass sample.
 Keywords
loop-mediated isothermal amplification;Salmonella spp.;screening;duck;
 Language
Korean
 Cited by
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Loop-mediated isothermal amplification assay for the rapid detection of swine influenza virus, Korean Journal of Veterinary Service, 2015, 38, 2, 107  crossref(new windwow)
2.
Visual detection of porcine circovirus 2 by loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye, Korean Journal of Veterinary Service, 2015, 38, 3, 145  crossref(new windwow)
3.
Application of loop-mediated isothermal amplification with propidium monoazide treatment to detect liveSalmonellain chicken carcasses, Poultry Science, 2016, pew341  crossref(new windwow)
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