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Antioxidative Changes of Blueberry Leaf Extracts in Emulsion-Type Sausage during In Vitro Digestion
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 Title & Authors
Antioxidative Changes of Blueberry Leaf Extracts in Emulsion-Type Sausage during In Vitro Digestion
Hur, Sun-Jin; Kim, Doo-Hwan; Chun, Se-Chul; Lee, Si-Kyung;
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This study was conducted to investigate the effects of in vitro human digestion on the antioxidant activity of blueberry leaf extracts (BLE) in emulsion-type sausages (ETS). Leaves from four cultivars of blueberries (Bluecrop, Bluegold, Duke, and Northland) collected from a wild blueberry farm were extracted with 80% ethanol. ETS were prepared with 0.2% BLE. The samples were then passed through an in vitro human digestion system which simulates the composition of the mouth, stomach, and small intestine juice. Only one phenolic compound (chlorogenic acid) was detected in the BLE. Northland BLE had appreciably higher amounts of chlorogenic acid than that of other BLE, both before and after in vitro human digestion. Antioxidant activity of any BLE was not influenced by in vitro human digestion, whereas the antioxidant activity of chlorogenic acid standard increased in response to in vitro human digestion in both 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric-reducing ability of plasma (FRAP). In the present study, the antioxidant activities of the BLE were not strongly influenced by in vitro human digestion, and the antioxidant activity depended on the chlorogenic acid content of ETS. Thus, compounds from blueberry leaves may have important applications in the future as natural antioxidants for meat products.
antioxidant activity;blueberry leaf;emulsion-type sausage;in vitro human digestion;
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Anonym. (2013). Blueberry variety review. Nate Nourse Nourse Farms. Accessed Aug. 21, 2013

Bermudez-Soto, M. J., Tomas-Barberan, F. A., and Garcia-Conesa, M. T. (2007). Stability of polyphenols in chokeberry (Aronia melanocarpa) subjected to in vitro gastric and pancreatic digestion. Food Chem. 102, 865-874. crossref(new window)

Ehlenfeldt, M. K., and Prior, R. L. (2001). Oxygen radical absorbance capacity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. J. Agr. Food Chem. 49, 2222-2227. crossref(new window)

Hur, S. J., Decker, E. A., and McClements, D. J. (2009). Influence of initial emulsifier type on microstructural changes occurring in emulsified lipids during in vitro digestion. Food Chem. 114, 253-262. crossref(new window)

Jeong, C. H., Choi, G. N., Kim, J. H., Kwak, J. H., Kim, D. O., Kim, Y. J., and Heo, H. J. (2010). Antioxidant activities from the aerial parts of Platycodon grandiflorum. Food Chem. 118, 278-282. crossref(new window)

Kim, S. M., Shang, Y. F., Um, B. H. (2010). Preparative separation of chlorogenic acid by centrifugal partition chromagography from highbush blueberry leaves (Vaccinium corymbosum L.). Phytochem. Anal. 21, 457-462. crossref(new window)

Marques, V., and Farah, A. (2009). Chlorogenic acids and related compounds in medicinal plants and infusions. Food Chem. 113, 1370-1376. crossref(new window)

McDougall, G. J., Fyffe, S., Dobson, P., and Stewart, D. (2005). Anthocyanins from red wine - Their stability under simulated gastrointestinal digestion. Phytochem. 66, 2540-2548. crossref(new window)

Molan, A. L., Lila, M. A., and Mawson, J. (2008). Satiety in rats following blueberry extract consumption induced by appetite-suppressing mechanisms unrelated to in vitro or in vivo antioxidant capacity. Food Chem. 107, 1039-1044. crossref(new window)

Naczk, M., Grant, S., Zadernowski, R., and Barre, E. (2006). Protein precipitating capacity of phenolics of wild blueberry leaves and fruits. Food Chem. 96, 640-647. crossref(new window)

Prior, R. L., Cao, G., Martin, A., Sofic, E., McEwen, J., O'Brien, C., Lischner, N., Ehlenfeldt, M., Kalt, W., Krewer, G., and Mainland, C. M. (1998). Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species. J. Agr. Food Chem. 46, 2686-2693. crossref(new window)

Sellappan, S., Akoh, C. C., and Krewer, G. (2002). Phenolic compounds and antioxidant capacity of georgia-grown blueberries and blackberries. J. Agr. Food Chem. 50, 2432-2438. crossref(new window)

Silberberg, M., Morand, C., Mathevon, T., Besson, C., Manach, C., Scalbert, A., and Remesy, C. (2006). The bioavailability of polyphenols is highly governed by the capacity of the intestine and of the liver to secrete conjugated metabolites. Eur J. Nutr. 45, 88-96. crossref(new window)

Stevenson, D. E., Cooney, J. M., Jensen, D. J., Zhang, J., and Wibisono, R. (2007). Comparison of the relative recovery of polyphenolics in two fruit extracts from a model of degradation during digestion and metabolism. Mol. Nutr. Food Res. 51, 939-945. crossref(new window)

Su, M. S. and Chien, P. J. (2007). Antioxidant activity, anthocyanins, and phenolics of rabbiteye blueberry (Vaccinium ashei) fluid products as affected by fermentation. Food Chem. 104, 182-187. crossref(new window)

Versantvoort, C. H. M., Oomen, A. G., Van de Kamp, E., Rompelberg, C. J. M., and Sips, A. J. A. M. (2005). Applicability of an in vitro digestion model in assessing the bioaccessibility of mycotoxins from food. Food Chem. Toxicol. 43, 31-40. crossref(new window)

Weber, C. (2012). Blueberry variety review. Cornell University Fruit Resources. Accessed Aug. 21, 2013.

Zheng, W., and Wang, S. Y. (2002). Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. J. Agr. Food Chem. 51, 502-509.