Publisher : Korean Society for Food Science of Animal Resources
DOI : 10.5851/kosfa.2013.33.6.696
Title & Authors
Detection of Meat Origin (Species) Using Polymerase Chain Reaction Park, Yong Hyun; Uzzaman, Md. Rasel; Park, Jeong-Woon; Kim, Sang-Wook; Lee, Jun Heon; Kim, Kwan-Suk;
A quick and reliable method for identifying meat origin is developed to ensure species origin of livestock products for consumers. The present study examined the identification of meat sources (duck, chicken, goat, deer, pig, cattle, sheep, and horse) using PCR by exploiting the mitochondrial 12S rRNA and mitochondrial cytochrome b genes. Species-specific primers were designed for some or all mitochondrial 12S rRNA nucleotide sequences to identify meat samples from duck, chicken, goat, and deer. Mitochondrial cytochrome b genes from pig, cattle, sheep, and horse were used to construct species-specific primers, which were used to amplify DNA from different meat samples. Primer sets developed in this study were found to be superior for detecting meat origin when compared to other available methods, for which the discrimination of meat origin was not equally applicable in some cases. Our new development of species-specific primer sets could be multiplexed in a single PCR reaction to significantly reduce the time and labor required for determining meat samples of unknown origin from the 8 species. Therefore, the technique developed in this study can be used efficiently to trace the meat origin in a commercial venture and help consumers to preserve their rights knowing origin of meat products for social, religious or health consciousness.
mtDNA 12S rRNA gene;cytochrome b gene;PCR;species-specific primer;multiplexing;meat (species) identification;
Chen, S. Y., Yao, Y. G., and Liu, Y. P. (2012) Species identification of ten common farm animals based on mitochondrial 12S rRNA gene polymorphisms. Anim. Biotechnol. 23, 213-220.
Chikuni, K., Tabata, T., Kosugiyama, M., Monma, M., and Saito, M. (1994a) Polymerase chain reaction assay for detection of sheep and goat meats. Meat Sci. 37, 337-345.
Chikuni, K., Tabata, T., Saito, M., and Monma, M. (1994b) Sequencing of mitochondrial cytochrome b genes for the identification of meat species. J. Anim. Sci. Technol. 65, 571-579.
Fei, S., Okayama, T., Yamanoue, M., Nishikawa, I., Mannen, H., and Tsuji, S. (1996) Species identification of meats and meat products by PCR. J. Anim. Sci. Technol. 67, 900-905.
Girish P. S., Anjaneyulu A. S. R., Viswas K. N., Anand M., Rajkumar N., Shivakumar B. M., and Sharma Bhaskar. (2004) Sequence analysis of mitochondrial 12S rRNA gene can identify meat species. Meat Sci. 66, 551-556.
Kesmen, Z., Sahin, F., and Yetim, H. (2007) PCR assay for the identification of animal species in cooked sausages. Meat Sci. 77, 1-5.
Matsunaga, T., Chikuni, K., Tanabe, R., Muroya, S., Shibata, K., Yamada, J., and Shinmura, Y. (1999) A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci. 51, 143-148.
Parson, W., Pegoraro, K., Niederstatter, H., Foger, M., and Steinlechner, M. (2000) Species identification by means of the cytochrome b gene. Int. J. Legal Med. 114, 23-28.