Advanced SearchSearch Tips
Antiobesity and Cholesterol-Lowering Effects of Bifidobacteria animalis DY-64 in Rats Fed a High-Fat/High-Cholesterol Diet
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Antiobesity and Cholesterol-Lowering Effects of Bifidobacteria animalis DY-64 in Rats Fed a High-Fat/High-Cholesterol Diet
Choi, Seong-Ho; Lee, Myung-Yul; Jhon, Deok-Young; Choi, Yang-Il; Lee, Jae-Joon;
  PDF(new window)
The present study was carried out to investigate the antiobesity and hypocholesterolemic effects of Bifidobacteria animalis DY-64 (B. animalis DY-64), a lactic acid bacterium isolated from the human intestine, in rats fed a high-fat/high-cholesterol diet for 4 weeks. Forty male Sprague-Dawley rats were divided into four groups and fed either a normal (N) or high-fat/high-cholesterol (HFC) diet without or with oral administration of B. animalis DY-64 (N-BA, HFC-BA). The gain in body weight, and liver and adipose tissue weights of the HFC group were heavier than that of the HFC-BA group. Serum total cholesterol (TC), LDL-cholesterol, and leptin levels of the HFC group, which were significantly elevated compared to those of the N group, dropped by 19, 18, 21, and 13% in the HFC-BA group, respectively, whereas the serum HDL-cholesterol level markedly increased. However, serum TG, LDL-cholesterol, HDL-cholesterol, and leptin levels were not significantly different among the N groups (N, N-BA) with or without B. animalis DY-64 administration. TC and TG levels of the liver as well as the TG level of the adipose tissue were significantly reduced in the HFC-BA group. In addition, HR-LPL activity in adipose tissue was also lower in the HFC-BA group than in the HFC group. These results suggest that B. animalis DY-64 isolated from the human intestine exerts hypocholesterolemic effects by reducing serum and liver cholesterol levels and plays a role in the prevention of obesity induced by HFC diet.
Bifidobacteria animalis DY-64;high-fat/high-cholesterol diet;obesity;cholesterol;rat;
 Cited by
An, H. M., Park, S. Y., Lee, D. K., Kim, J. R., Cha, M. K., Lee, S. W., Lim, H. T., Kim, K. J., and Ha, N. J. (2011) Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats. Lipids Health Dis. 10, 116-124. crossref(new window)

Biggs, H. G., Erikson, T. M., and Moorehead, W. R. (1975) A manual colorimetric assay of triglyceride in serum. Clin. Chem. 21, 437-441.

Considine, R. V., Shinha, M. K., and Heiman, M. L. (1996) Serum immunoreactive leptin concentrations in normal weight and obese humans. New Engl. J. Med. 334, 292-295. crossref(new window)

Cruz, M. L. and Williamson, D. H. (1992) Refeeding meal-fed rats increased lipoprotein lipase activity and deposition of dietary [$^{14}C$] lipid in white adipose tissue and decrease oxidation to $^{14}CO_2$. Biochem. J. 285, 773-778.

Donohue, D. C. (2006) Safety of probiotics. Asia. Pac. J. Clin. Nutr. 15, 563-569.

Eckel, R. H. (1989) Lipoprotein lipase: A multifunctional enzyme relevant to common metabolic disease. New Eng. J. Med. 320, 1060-1068. crossref(new window)

Folch, J., Lees, M., and Sloane-Stanley, G. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497-509.

Fried, S. K., Turkenkopf, I. J., Goldberg, I. J., Doolittle, M. H., Kirchgessner, T. G., Schotz, M. C., Johnson, P. R., and Greenwood, M. R. C. (1991) Mechanisms of increased lipoprotein lipase in fat cells of obese Zucker rats. Am. J. Physiol. 261, E653-E660.

Friedman, J. M. (2002) The function of leptin in nutrition, weight, and physiology. Nutr. Rev. 60, S1-S14.

Friedwald, W., Levy, R., and Fredrickson, D. (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18, 499-502.

Fuller, R. (1992) History and development of probiotics. Probiotics-The Scientific Basis Champman and Hall, London: Fukker, R. 1-18.

Garrow, J. S. (1992) Treatment of obesity. Lancet 340, 409-413. crossref(new window)

Haslam, D. W. (2005) Obesity. Lancet 366, 1197-1209. crossref(new window)

Iwashita, S., Tandia, M., Terui, N., Ootsuka, Y., Shu, M., Kang, D. (2002) Direct measurement of renal sympathetic nervous activity in high-fat diet-related hypertensive rats. Life Sci. 71, 537-546. crossref(new window)

Lee, D. K., Jang, S., Baek, E. H., Kim, M. J., Lee, K. S., Shin, H. S., Chung, M. J., Kim, J. E., Lee, K. O., and Ha, N. J. (2009) Lactic acid bacteria affect serum cholesterol levels, harmful enzyme activity, and fecal water content. Lipids Health Dis. 8, 21-19. crossref(new window)

Lee, H. Y., Park, J. H., Seok, S. H., Baek, M. W., Kim, D. J., Lee, K. E., Paek, K. S., Lee, Y. H., and Park, J. H. (2006) Human originated bacteria, Lactobacillus rhamnous PL60, produce conjugated linoleic acid and show anti-obesity effects in diet-induced obese mice. Biochim. Biophys. Acta. 1761, 736-744. crossref(new window)

Lee, K., Paek, K., Lee, Y. H., Park, J. H., and Lee, Y. (2007) Antiobesity effect trans-10, cis-12-conjugated linoleic acid-producing Lactobacillus planetarium PL62 on diet-induced obese mice. J. Appl. Microbiol. 103, 1140-1146. crossref(new window)

Nettleton, J. A., Polak, J. F., Tracy, R., Burke, G. L., and Jacobs Jr, D. R. (2009) Dietary patterns and incident cardiovascular disease in the multi-ethnic study of atherosclerosis. Am. J. Clin. Nutr. 90, 647-654. crossref(new window)

Nicolosi, R. J., Wilson, T. A., Lawton, C., and Handelman, G. J. (2001) Dietary effects on cardiovascular disease risk factors: beyond saturated fatty acids and cholesterol. J. Am. Coll. Nutr. 20, 421-427. crossref(new window)

Nilsson-Ehle, P and Schotz, M. A. (1976). A stable, radioactive substrate emulsion for assay of lipoprotein lipase. J. Lipid Res. 17, 536-541.

Reeves, P. G., Nielson, F. H., and Fahey Jr, G C. (1993) AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition adhoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123, 1939-1951.

Ong, Z. M. and Kern, P. A. (1989) Effect of feeding and obesity on lipoprotein lipase activity, immunoreactive protein, and messenger RNA levels in human adipose tissue. J. Clin. Invest. 84, 305-311. crossref(new window)

Rosenfeld, L. (1989) Lipoprotein analysis. Arch. Pathol. Lab. Med. 113, 1101-1110.

Taylor, G. R. J. and Williams, C. M. (1998) Effects of probiotics and prebiotics on blood lipids. Br. J. Nutr. 80, S225-S230.

Xiao, J. Z., Kondo, S., Takahashi, N., Oshida, K., Hiramatsu, A., Iwatsuki, K., Kobubo, S., and Hosono, A. (2003) Effects of milk products fermented by Bifidibaterium longum on blood lipids in rats and healthy adult male volunteers. J. Dairy Sci. 86, 2452-2461. crossref(new window)

Xie, N., Cui, Y. N., Zhao, X., Yang, Z. W., Wang, Z. G., Fu, N., Tang, Y., Wang, X. H., Liu, X. W., Wang, C. L., Lu, F. G. (2011) Effects of two Lactobacillus strains on lipid metabolism and intestinal microflora in rats fed a high-cholesterol diet. BMC Complemetary Alternative Med. 11, 53-63. crossref(new window)

Yin, Y. N., Yu, Q. F., Fu, N., Liu, X. W., and Lu, F. G. (2010) Effects of four Bifidobacteria on obesity in high-fat diet induced rats. World J. Gastroenterol. 16, 3394-401. crossref(new window)

Zlatkis, A. and Zak, B. (1969) Study of a new cholesterol reagent. Anal. Biochem. 29, 143-148. crossref(new window)