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Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection
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 Title & Authors
Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection
Yoo, Jinhee; Park, Kimoon; Yoo, Youngji; Kim, Jongkeun; Yang, Heejin; Shin, Youngjae;
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This study was conducted to examine the effects of eggshell membrane hydrolysates (ESMH) on the anti-inflammatory, anti-wrinkle, anti-microbial activity, and moisture-protection for cosmetic use. Whole ESMH (before fractionation), and fraction I (>10 kDa), fraction II (3-10 kDa), and fraction III (<3 kDa) of the hydrolysates were assessed in this experiment. As lipopolysaccharide (LPS) and IFN- caused the inflammation on Raw264.7 cell, whole ESMH and fraction I showed to be effective in inhibiting the induction of cell inflammation depending on the concentration, and also showed outstanding effect to suppress the skin inflammation. Fraction I inhibited collagenase and elastase activities to a greater extent than the other fractions, while all fractions had antibiotic effects at concentrations of 10 mg/disc and 20 mg/disc. In addition, it showed the moisture protection effects of skin on the holding amount and losing amount of moisture in upper-inner arm of the human body with a relatively low loss rate in skin, which confirmed that the hydrolyzed fractions of ESM helps to form the superior protective layer of moisture. It was concluded that ESMH fractions with different molecular weights, especially the 10 kDa fraction, have anti-lipopolysaccharide, anti-IFN--induced inflammation, anti-collagenase and elastase activities, and thus can be used as a cosmetic agent to protect skin.
eggshell membrane;anti-inflammation;anti-aging;antibiotic;moisture protection;
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Antonicelli, F., Bellon, G., Debelle, L., and Hornebeck, W. (2007) Elastin-elastases and inflamm aging. Curr. Top. Dev. Biol. 79, 99-155. crossref(new window)

Ashton, D. H. and Busta, F. F. (1968) Relief of casein inhibition of Bacillus stearothermophilus by iron, calcium, and magnesium. Appl. Microbiol. 16, 628-635.

Blanken, R., Van Vilsteren, M. J. T., Tupker, R. A., and Conraads, J. (1989) Effect of mineral oil and linoleic-acid-containing emulsions on the skin vapour loss of sodium-lauryl sulphate-induced irritant skin reactions. Contact Dermatitis 20, 93-97. crossref(new window)

Bredt, D. S. and Snyder, S. H. (1994) Nitric oxide: A physiologic messenger molecule. Annu. Rev. Biochem. 63, 175-195. crossref(new window)

Candilish, J. K. and Scougall, R. K. (1969) L-5-hydroxylysine as aconstituent of the shell membrane of the hen's egg. Int. J. Protein Res. 1, 299-306.

Carmichael, W. W. (1988) Toxins of freshwater algae, In A. T. Tu (ed.). Handbook of natural toxins, Marine toxins and venoms. Marcel Dekker, Inc., New York. 3, 121-147.

Chan, E. S., Fernandez, P., Merchant, A. A., Montesinos, M. C., Trzaska, S., Desai, A., Tung, C. F., Khoa, D. N., Pillinger, M. H., Reiss, A. B., Tomic-Canic, M., Chen, J. F., Schwarzschild, M. A., and Cronstein, B. N. (2006) Adenosine A2A receptors in diffuse dermal fibrosis: Pathogenic role in human dermal fibroblasts and in a murine model of scleroderma. Arthritis Rheum. 54, 2632-2642. crossref(new window)

Chiou, W. F., Chou, C. J., and Chen, C. F. (2001) Camptothecin suppresses nitric oxide biosynthesis in Raw264.7 macrophages. Life Science 69, 625-635. crossref(new window)

Griffiths, C. E., Russman, A. N., and Majmudar, G. (1993) Restoration of collagen formation in photodamaged human skin by tretinoin (retinoic acid). New Engl. J. Med. 329, 530-535. crossref(new window)

Grubauer, G., Elias, P. M., and Feingold, K. R. (1989) Transepidermal water loss: The signal for recovery of barrier structure and function. J. Lipid Res. 30, 323-333.

Haratake, A., Uchida, Y., and Schmuth, M. (1998) UVB-induced alterations in permeability barrier function: Roles for epidermal hyperproliferation and thymocyte-mediated response. J. Invest. Dermatol. 108, 769-775.

Jacobi, O. K. (1959) About the mechanism of moisture regulation in the horny layer of skin. Proc. Sci. Sec. Toilet. Goods Assoc. 31, 22-24.

James, A. E. K., Timothy, D. W., and Gorden, L. (1996) Inhibition of human leujocyte and porcine pancreatic elastase by homologues of bovine pancreatic tyrosinase inhibitors. Biochemistry 35, 9090-9096. crossref(new window)

Jiang, S. J., Chu, A. W., Lu, Z. F., Pan, M. H., Che, D. F., and Zhou, X. J. (2007) Ultraviolet B-induced alterations of the skin barrier and epidermal calcium gradient. Exp. Dermatol. 16, 985-992. crossref(new window)

Kang, H. H. (1997) Anti-aging in cosmetics. J. Soc. Cosmet. Scientists Korea 23, 57-73.

Masaki, H. (2010) Role of antioxidants in the skin: Antiaging effects. J. Dermatol. Sci. 58, 85-90. crossref(new window)

Middleton, J. D. (1974) Development of a skin cream designed to reduce dry and flaky skin. J. Soc. Cosmet. Chem. 25, 519-534.

Nagase, H., Visse, R., and Murphy, G. (2006) Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc. Res. 69, 562-573. crossref(new window)

Nathan, C. (1997) Inducible nitric oxide synthase: What difference does it make? J. Clin. Invest. 94, 5243-5248.

Ohara, H., Ito, K., Iida, H., and Matsumoto, H. (2009) Improvement in the moisture content of the stratum corneum following 4 weeks of collagen hydrolysate ingestion. Food Sci. Tech. Res. 56, 137-145 crossref(new window)

Oram, J. D. and Reiter, B. (1968) Inhibition of bacteria by lactoferrin and other iron-chelating agents. Biochem. Biophys. Acta. 170, 351-365. crossref(new window)

Park, C. S. (2008) Skin barrier and beauty foods. Food Sci. Indus. 40, 19-26.

Park, J. G., Hyun, J. W., Lim, K. H., Shin, J. E., Won, Y. J., Yi, Y. D., Shin, K. H., Chang, I. M., and Woo, W. S. (1993) Antineoplastic effects from traditional medicinal plants. Kor. J. Pharmacogn. 24, 223-230.

Park, K. M., Yoo, J. H., and Shin, Y. G. (2012) Effects of egg shell membrane hydrolysates on skin whitening, wound healing, and UV-protection. Korean J. Food Sci. An. 32, 308-315. crossref(new window)

Pillai, S., Oresajo, C., and Hayward, J. (2005) Ultraviolet radiation and skin aging: Roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - A review. Int. J. Cosmet. Sci. 27, 17-34. crossref(new window)

Prall, J. K. (1966) Scaliness of human skin. Archives De Biochemie Et Cosmetologie. 9, 87-88.

Rittié, L. and Fisher, G. J. (2002) UV-light-induced signal cascades and skin aging. Ageing Res. Rev. 1, 705-720. crossref(new window)

Roskos, K. V. and Guy, R. H. (1989) Assessment of skin barrier function using transepidermal water loss: Effect of age. Pharm Res. 6, 949-953. crossref(new window)

Sorg, O., Antille, C., Kaya, G., and Saurat, J. H. (2006) Retinoids in cosmeceuticals, Dermatol. Ther. 19, 289-296. crossref(new window)

Tsuji, N., Moriwaki, S., Suzuki, Y., Takema,Y., and Imokawa, G. (2001) The role of elastases secreted by fibroblasts in wrinkle formation: Implication through selective inhibition of elastase activity. Photochem. Photobiol. 74, 283-290. crossref(new window)

Tsukahara, K., Nakagawa, H., Moriwaki, S., Takema, Y., Fujimura, T., and Imokawa, G. (2006) Inhibition of ultraviolet- B-induced wrinkle formation by an elastase-inhibiting herbal extract: Implication for the mechanism underlying elastaseassociated wrinkles. Int. J. Dermatol. 45, 460-480. crossref(new window)

Tsukahara, K., Takema, Y., Moriwaki, S., Tsuji, N., Suzuki, Y., and Fujimura, T. (2001) Selective inhibition of skin fibroblast elastase elicits a concentration - dependent prevention of ultraviolet B-induced wrinkle formation. J. Invest. Dermatol. 117, 671-677. crossref(new window)

Vignardet, C. G., Friedrich, Y. C., and Millet, J. (1999) A first order experiment design to assess soluble proteins released by a new keratinase from Doratomyces microsporus on human substrate. Int. J. Pharm. 191, 95-102. crossref(new window)

Willoughby, D. A. (1974) Human arthritis applied to animal models. Towards a better therapy. Ann. Rheum. Dis. 34, 471-478.

Yi, F., Guo, Z. X., Zhang, L. X., Yu, J., and Li, Q. (2004) Soluble eggshell membrane protein: preparation, characterization and biocompatibility. Biomaterials 25, 4591-4599. crossref(new window)