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Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats
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 Title & Authors
Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats
Nurliyani, Nurliyani; Kandarina, B.J. Istiti; Kusuma, Sari; Trisnasari, Yunita Dewi;
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Total spleen lymphocytes, lymphocyte proliferation, tumor necrosis factor- (TNF-), and interleukin-10 (IL-10) in spleen lymphocyte culture were studied in malnourished Wistar rats fed with goat milk yoghurt. Malnourished rats were created by using standard feed restriction as much as 50% of normal rats for 21 d. Goat milk yoghurt containing three types of microorganism e.g., Lactobacillus acidophilus, Sterptococcus thermophilus and Bifidobacterium longum derived from Lacto-B culture in powder form. After 21 d, the rats continued to receive restricted feeding and supplemented with goat milk yoghurt for 7 d. Total splenocytes were counted by hemocytometer. Splenocytes proliferation was expressed as stimulation index, whereas the TNF- and IL-10 of spleen lymphocyte culture were measured by ELISA technique. The total number of splenocytes and stimulation index of splenocytes in moderate malnourished and normal rats supplemented with goat milk yoghurt was not significantly different. The level of TNF- in the rat supplemented with goat milk yoghurt was lower (p<0.05) than the control group, whereas the level of IL-10 in the rat supplemented with goat milk yoghurt was higher (p<0.05) than the control group. In conclusion, goat milk yoghurt supplementation in malnourished rats could decrease TNF- as a representation of the pro-inflammatory cytokine, while it increases IL-10 as a representation of the anti-inflammatory cytokine.
goat milk yoghurt;IL-10;lymphocyte proliferation;malnutrition;TNF-;
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Al-Bana, N., Raghupathy, R., and Albert, M. J. (2008) Correlation of proinflammatory and anti inflammatory cytokine levels with histopathological changes in an adult mouse lung model of Campylobacter jejuni infection. Clin. Vaccine Immunol. 15, 1780-1787. crossref(new window)

Alferez, M. J. M, Barrionuevo, M., Aliaga, I. L., Sanz-Sampelayos, M. R., Lisbona, F., Robles, J. C., and Campos, M. S. (2001) Digestive utilization of goat and cow milk fat in malabsorption syndrome. J. Dairy Res. 68, 451-461.

Arora, R., Bhojak, N., and Joshi, R. (2013) Comparative aspects of goat and cow milk. IJESI. 2, 7-10.

Arribas, B., Rodriguez-Cabezas, M. E., Camuesco, D., Comalada, M., Bailon, E., Utrilla, P., Nieto, A., Concha, A., Zarzuelo, A., and Galvez, J. (2009) A probiotic strain of Escherichia coli, Nissle 1917, given orally exerts local and systemic antiinflammatory effects in lipopolysaccharide-induced sepsis in mice. Br. J. Pharmacol. 157, 1024-1033. crossref(new window)

Baroja, M. L., Kirjavainen, P. V., Hekmat, S., and Reid, G. (2007) Anti-inflammatory effects of probiotic yogurt in inflammatory bowel disease patients. Clin. Exp. Immunol. 149, 470-479. crossref(new window)

Bode, L. (2006) Recent advances on structure, metabolism, and function of human milk oligosaccharides. J. Nutr. 136, 2127-2130.

Borruel, N., Carol, M., Casellas, F., Antolin, M, de Lara, F., Espin, E., Naval, J., Guarner, F., and Malagelada, J. R. (2002) Increased mucosal tumour necrosis factor-$\alpha$ production in Crohn's disease can be downregulated ex vivo by probiotic bacteria. Gut 51, 659-664. crossref(new window)

Cesta. (2006) Normal structure, function and histology of the spleen. Toxicol Pathol. 34, 455-465. crossref(new window)

Cheung, H. T. and Nadakavukaren, M. J. (1983) Age-dependent changes in the cellularity and ultrastructure of the spleen of Fischer F344 rats. Mech Ageing Dev. 22, 23-33. crossref(new window)

Chi, D. S., Berry, D. L., Dillon, K. A., and Arbogast, B. W. (1982) Inhibition of in vitro lymphocyte response by streptozotocin- induced diabetic rat serum. A Function of Very-Low- Density Lipoproteins. Diabetes 37, 1098-1104.

Correia, M., Cravo, M., Marques-Vidal, P., Grimble, R., Dias- Pereira, A., Faias, S., Nobre-Leitao, C. (2007) Serum concentrations of TNF-alpha as a surrogate marker for malnutrition and worse quality of life in patients with gastric cancer. J. Clin. Nutr. 26, 728-735. crossref(new window)

Cortes-Barberena, E., Gonzalez-Marquez, H., Gomez-Olivares, J. L., and Ortiz-Muniz, R. (2008) Effects of moderate and severe malnutrition in rats on splenic T lymphocyte subsets and activation assessed by flow cytometry. Clin. Exp. Immunol. 152, 585-592. crossref(new window)

Corthesy, B., Gaskins, H. R., and Mercenier, A. (2007) Crosstalk between probiotic bacteria and the host immune system. J. Nutr. 137, 781S-790S.

Daddaoua, A., Puerta, V., Requena, P., Martinez, F. A., Guadix, E., Sanches de M. F., Zarzuel, A., Suarez, D. M., Boza, J. J., and Martinez, A. O. (2006) Goat milk oligosaccharides are anti-inflammatory in rats with hapten-induced colitis. J. Nutr. 136, 672-676.

Dask, S. K. (2009). Selection Criteria for Probiotics. Presented at XXXVII Dairy Industry Conference, Kala Academy, Panjim, Goa.

Dave, R. I. (1998). Factors affecting viability of yoghurt and probiotic bacteria in commercial starter cultures. A thesis submitted for the degree of Doctor of Philosophy. Centre for Bioprocessing and Food Technology, School of Life Sciences and Technology,Victoria University of Technology, Werribee Campus, Victoria, Australia.

de LeBlanc, A. M., del Carmen, S., Zurita-Turk, M., Rocha, C. S., van de Guchte, M., Azevedo, V., Miyoshi, A., and Le- Blanc, J. G. (2011) Importance of IL-10 modulation by probiotic microorganisms in gastrointestinal inflammatory diseases. ISRN Gastroenterol. 11, 1-11.

de LeBlanc, A. M. and Perdigon, G. (2010) The application of probiotic fermented milks in cancer and intestinal inflammation. P. Nutr. Soc. 69, 421-428. crossref(new window)

Dock, D. B., Agular-Nascimento, J. E., and Latorraca, M. Q. (2004) Probiotics enhance the recovery of gut atrophy in experimental malnutrition. Biocell 28, 143-150.

Franca, T. G. D., Ishikawa, L. L. W., Zorzella-Pezavento, S. F. G., Chiuso-Minicucci, F., da Cunha, M. L. R. S., and Sartori, A. (2009) Impact of malnutrition on immunity and infection. J. Venom. Anim. Toxins Incl. Trop. Dis. 15, 374-390. crossref(new window)

Galdeano, C. M. and Perdigon, G. (2006) The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity. Clin Vaccine Immunol . 13, 219-226. crossref(new window)

Galdeano, C. M., Nunez, I. N., de LeBlanc, A. M., Carmuega, E., Weill, R., and Perdigon, G. (2011) Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice. BMC Gastroenterol. 11, 6-14. crossref(new window)

Giacinto, C. D., Marinaro, M., Sanchez, M., Strober, W., and Boirivant, M. (2005) Probiotics ameliorate recurrent Th1- mediated murine colitis by inducing IL-10 and IL-10-dependent TGF-$\beta$-bearing regulatory cells. J. Immunol. 174, 3237- 3246. crossref(new window)

Grabig, A., Paclik, D., Guzy, C., Dankof, A., Baumgart, D. C., and Erckenbrecht, J. (2006). Escherichia coli strain Nissle 1917 ameliorates experimental colitis via toll-like receptor 2- and toll-like receptor 4-dependent pathways. Infect Immun. 74, 4075-4082. crossref(new window)

Hockertz, S. (1997) Augmentation of host defence against bacterial and fungal infections of mice pretreated with the non-pathogenic Escherichia coli strain Nissle 1917. Arzneimittelforschung 47, 793-796.

Hubert, M. F., Laroque, P., Gillet, J. P., and Keenan, K. P. (2000) The effect of diet, ad libitum feeding, and moderate and severe dietary on body weight, survival, clinical pathology parameters, and cause of death in control Sprague Dawley rats. Toxicol. Sci. 58, 195-207. crossref(new window)

Itoh, T., Miyake, Y., Onda, A., Kubo, J., Ando, M., Tsukamasa, Y., and Takahata, M. (2012) Immunomodulatory effects of heat-killed Enterococcus faecalis TH10 on murine macrophage cells. MicrobiologyOpen 1, 373-380. crossref(new window)

Jain, S., Yadav, H., Sinha, P. R., Naito, Y., and Marotta, F. (2008) Dahi containing probiotic Lactobacillus acidophilus and Lactobacillus casei has a protective effect against Salmonella enteridis infection in mice. Int. J. Immunopathol. Pharmacol. 21, 1021-1029.

Keller, J. M., McClellan-Greena, P. D., Lee, A. M., Arendt, M. D., Maier, P. P., Segars, A. L., Whitaker, J. D., Keil, D. E., and Peden-Adams, M. M. (2005) Mitogen-induced lymphocyte proliferation in loggerhead sea turtles: Comparison of methods and effects of gender, plasma testosterone concentration, and body condition on immunity. Vet. Immunol. Immunopathol. 103, 269-281. crossref(new window)

Kirjavainen, P. V., Elnezami, H. S., Salminen, S. J., Ahokas, J. T., and Wright, P. F. A. (1999) Effects of orally administered viable Lactobacillus rhamnosus GG and Propionibacterium freudenreichii subsp. Shermanii JS on mouse lymphocyte proliferation. Clin. Diagn. Lab. Immunol. 799-802.

Kompan, D. and Komprej, A. (2012) The effect of fatty acids in goat milk on health. In: Milk production - An up-to-date overview of animal nutrition, management and health. Chaiyabutr, N. (ed) InTech, Croatia, pp. 1-26.

Kuhn, R., Lohler, J., Rennick, D., Rajewsky, K., and Muller, W. (1993) Interleukin-10-deficient mice develop chronic enterocolitis. Cell 75, 263-274. crossref(new window)

Kwon, H. K., Lee, C. G., So, J. S., Chae, C. S., Hwang, J. S., Sahoo, A., Nam, J. H., Rhee, J. H., Hwang, K. C., and Im, S. H. (2010) Generation of regulatory dendritic cells and CD4+ Foxp3+ T cells by probiotics administration suppresses immune disorders. PNAS. 107, 2159-2164. crossref(new window)

Lara-Villoslada, F., Debras, E., Nieto, A., Concha, A., Galvez, J., Lopez-Huertas, E., Boza, J., Obled, C., and Xaus, J. (2006) Oligosaccharides isolated from goat milk reduce intestinal inflammation in a rat model of dextran sodium sulfate- induced colitis. Clin. Nutr. 25, 477-488. crossref(new window)

Mantovani, G., Madeddu, C., Maccio, A., Gramignano, G., and Lusso, M. (2004) Cancer-related anorexia/cachexia syndrome and oxidative stress: An innovative approach beyond current treatment. Canc. Epidem. Biomark. Prev. 13, 1651- 1659.

Martinez-Ferez, A., Rudloff, S., Guadix, A., Henkel, C. A., Pohlentz, G., Boza, J. J., Guadix, E. M., and Kunz, C. (2006) Goats' milk as a natural source of lactose-derived oligosaccharides: Isolation by membrane technology. Int. Dairy J. 16, 173-181. crossref(new window)

Mengheri, E. (2008) Health, probiotics, and inflammation. J. Clin. Gastroenterol. 42, S177-178. crossref(new window)

Meydani, S. N. and Ha, W. K. (2000) Immunologic effects of yogurt. Am. J. Clin. Nutr. 71, 861-872.

Nunez, I. N, Galdeano, C. M., Carmuega, E., Weill, R., de Le- Blanc, A. M., and Perdigon, G. (2013) Effect of a probiotic fermented milk on the thymus in Balb/c mice under non-severe protein-energy malnutrition. Br. J. Nutr. 110, 500-508. crossref(new window)

Ortiz, R., Cortes, L., Cortes, E., and Medina, H. (2008) Malnutrition alters the rates of apoptosis in splenocytes and thymocyte subpopulations of rats. Clin. Exp. Immunol. 155, 96-106.

Palacios, M. G., Cunnick, J. E., Winkler, D. W., and Vleck, C. M. (2007) Immunosenescence in some but not all immune components in a free-living vertebrate, the tree swallow. Proc. R. Soc. 274, 951-957. crossref(new window)

Park, Y. W. (1994) Hypo-allergenic and therapeutic significance of goat milk. Small Ruminant Res. 14, 151-159. crossref(new window)

Pavlovic, H., Hardi, J., Slacanac, V., Halt, M., and Kocevski, D. (2006) Inhibitory effect of goat and cow milk fermented by Bifidobacterium longum on Serratia marcescens and Campylobacter jejuni. Czech J. Food Sci. 24, 164-171.

Perdue, M. H. (1999) Mucosal immunity and inflammation III. The mucosal antigen barrier: cross talk with mucosal cytokines. AM J. Physiol-Gastr L. 227, G1-G5.

Prada, F. J. A., Macedo, D. V., and de Melo, M. A. R. (2007) Oxidative stress during rehabilitation from protein malnutrition associated with aerobic exercise in rats. Braz. Arch. Biol. Technol. 50, 45-55. crossref(new window)

Ramiro-Puig, E., Perez-Cano, F. J., Ramirez-Santana, C., Castellote, C., Izquierdo-Pulido, M., Permanyer, J., Franch, A., and Castell, M. (2007) Spleen lymphocyte function modulated by a cocoa-enriched diet. Clin. Exp. Immunol. 149, 535-542. crossref(new window)

Razafindrakoto, O., Ravelomanana, N., Rasolofo, A., Rakotoarimanana, R. D., Gourgue, P., Coquin, P., Briend, A., and Desjeux, J. F. (1993) Le lait de chevre peut-il remplacer le lait de vachechez l'enfant malnutri? Lait 73, 601-611. crossref(new window)

Reeves, P. G., Neilsen, F. H., and Fahey, Jr. G. C. (1993) AIN- 93 purified diets for laboratory rodents: Final report of the American institute of nutrition Ad Hoc writing committee on the formulation of the AIN-76A Rodent Diet. J. Nutr. 123, 1939-1951.

Riskesdas. (2010) Riset Kesehatan Dasar. Kementerian Kesehatan Republik Indonesia.

Roselli, M., Finamore, A., and Nuccitelli, S. (2009) Prevention of TNBS-induced colitis by different Lactobacillus and Bifidobacterium strains is associated with an expansion of ${\gamma}{\delta}T$ and regulatory T cells of intestinal intraepithelial lymphocytes. Inflamm. Bowel Dis. 15, 1526-1536. crossref(new window)

Rosso, P., Keyou, G., Bassi, J. A., and Slusser, W. M. (1981) Effect of malnutrition during pregnancy on the development of the mammary glands of rats. J. Nutr. 111, 1937-1941.

Sartor, R. B. (2011). Efficacy of Probiotics for the management of inflammatory bowel disease. J Gastroen Hepatol. 7, 606-608.

Savino, W. (2002) The thymus gland is a target in malnutrition. Eur. J. Clin. Nutr. 56, S46-S49.

Semaeva, E., Tenstad, O., Skavland, J., Enger, M., Iversen, P. O., Gjertsen, B. T., and Wiig, H. (2010) Access to the spleen microenvironment through lymph shows local cytokine production, increased cell flux, and altered signaling of immune cells during lipopolysaccharide-induced acute inflammation. J. Immunol. 184, 4547-4556. crossref(new window)

Sfeir, R. M., Dubarry, M., Boyaka, P. N., Rautureau, M., and Tome, D. (2004) The mode of oral bovine lactoferrin administration influences mucosal and systemic immune reponses in mice. J. Nutr. 134, 403-409.

Shimazaki, K., Tazume, T., Uji, K., Tanaka, M., Kumura, H., Mikawa, K., and Shimo-Oka, T. (1998) Properties of a heparin- binding peptide derived from bovine lactoferrin. J. Dairy Sci. 81, 2841-2849. crossref(new window)

Silankove, N., Leitner, G., Merin, U., and Prosser, C. G. (2010) Recent advances in exploiting goat’s milk: Quality, safety and production aspects. Small Ruminant Res. 89, 110-124. crossref(new window)

SPSS. (2003) Statistical package for social sciences for windows (version 12.0). Chicago, IL: SPSS Inc.

Sunarlim, R. (2009) Potensi Lactobacillus, sp asal dari dadih sebagai starter pada pembuatan susu fermentasi khas Indonesia. Buletin Teknologi Pascapanen Pertanian. 5, 69-76.

Takeda, K. and Akira, S. (2005) Toll-like receptors in innate immunity. Int. Immunol. 17, 1-14.

Van Furt, A. M., Roord, J. J., and van Furth R. (1996) Roles of Proinflammatory and Anti-Inflammatory cytokines in pathophysiology of bacterial meningitis and effect of adjunctive therapy. Infect. Immun. 64, 4883-4890.

Ward, P. P., Uribe-Luna, S., and Conneely, O. M. (2002) Lactoferrin and host defense. Biochem. Cell Biol. 80, 95-102. crossref(new window)

WHO. (2006) Food and nutrition policy for schools: A tool for the development of school nutrition programmes in the European Region. Programme for Nutrition and Food Security. WHO Regional Office for Europe. Copenhagen.

Wu, F. Y., Tsao, P. H., Wang, D. C., Lin, S., Wu, J., and Cheng, Y. K. (2006) Factors affecting growth factor activity in goat milk. J. Dairy Sci. 89, 1951-1955. crossref(new window)

Zeuthen, L. H., Fink, L. N., and Frøkiær, H. (2008) Toll-like receptor 2 and nucleotide-binding oligomerization domain-2 play divergent roles in the recognition of gut-derived lactobacilli and bifidobacteria in dendritic cells. J. Immunol. 124, 489-502. crossref(new window)

Ziegler, S. F. (2006) FOXP3: Of Mice and Men. Annu. Rev. Immunol. 24, 209-226. crossref(new window)

Zimecki, M. and Kruzel, M. L. (2000) Systemic or local coadministration of lactoferrin with sensitizing dose of antigen enhanches delayed type hypersensitivity in mice. Immunol Lett. 74, 183-188. crossref(new window)