JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG
Baek, Kyung Hoon; Park, Sung Kwon; Lee, Myung Hoon; Kim, Sung Il; Cho, Soo Hyun; Choi, Chang Bon;
  PDF(new window)
 Abstract
This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibody by ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those from non-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amount of antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat can be more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brands in the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoo brand beef.
 Keywords
Hanwoo;BIO-TAG;synthetic peptide;rapid diagnosis kit;
 Language
English
 Cited by
 References
1.
Baek, K. H., Choi, C. W., Choi, C. B., and Lee, B. S. (2007) Passive immunization approach to reduce body fat in pigs using fat-specific polyclonal antiserum. Asian-Aust. J. Anim. Sci. 20, 1594-1599. crossref(new window)

2.
Baek, K. H., Kwak, T. H., Oh, Y. S., Choi, C. W., Jung, K. K., and Choi, C. W. (2005) Studies on the development and utilization of polyclonal antibodies against swine adiocyte plasma membrane proteins. J. Anim, Sci. Technol (Kor.). 47, 19-28. crossref(new window)

3.
Benkirane, N., Guichard, G., Van Regenmortel, M. H. V., Briand, J., and Muller, S. (1995) Cross-reactivity of antibodies to retro-inverso peptidomimetics with the parent protein histone H3 and chromatin core particle. J. Biol. Chem. 270, 11921-11926. crossref(new window)

4.
Briand, J. P., Benkirane, N., Guichard, G., Newman, J. F. E., Van Regenmortel, M. H. V., Brown, F., and Muller, S. (1997) A retro-inverso peptide corresponding to the GH loop of footand-mouth disease virus elicits high levels of long-lasting protective neutralizing antibodies. Proc. Natl. Acad. Sci. 94, 12545-12550. crossref(new window)

5.
Cho, H. W. and Ahn, B. H. (2006) Effects of dietary vitamin E levels on carcass characteristics, vitamin E concentration of Longissimus muscle and lipid oxidation in Hanwoo steers. J. Anim. Sci. Technol. (Kor.). 48, 827-838. crossref(new window)

6.
Cho, W. M., Cho, Y. M., Hong, S. K., Jeong, E. S., Lee, J. M., and Yoon, S. K. (2000) Effects of feeding whole crop barley silage on growth performance, feed efficiency and meat quality in Hanwoo steers. J. Anim. Sci. Technol. (Kor.). 42, 181-188.

7.
Choi, C. W., Baek, K. H., Choi, C. B., Oh, Y. K., and Hong, S. G. (2010) Development of polyclonal antibodies to abdominal and subcutaneous adipocytes for producing fat-reduced high quality pork. Korean J. Food Sci. An. 30, 87-94. crossref(new window)

8.
Choi, C. W., Kim, Y. H., Kim, S. J., Song, M. G., Kwon, E. G., Oh, Y. K., Hong, S. G., Choi, S. H., and Baek, K. H. (2008) Development of polyclonal antibodies to abdominal and subcutaneous adipocytes for fat-reduced Hanwoo beef production. Korean J. Food Sci. An. 28, 651-659. crossref(new window)

9.
Chung, E. R. and Chung, K. Y. (2004) Identification of beef breed using DNA marker of coat color genes. Korean J. Food Sci. An. 24, 355-360.

10.
IEDB (Immune Epitope Database) and Analysis Resource (2012) User Documentation. La Jolla Institute for Allergy and Immunology. 9420 Athena Circle, La Jolla, CA 92037, USA. Available from: http://tools.immuneepitope.org/main/index.html

11.
Kim, D. G., Jung, K. K., Sung, S. K., Choi, C. B., Kim, S. G., Kim, D. Y., and Choi, B. J. (1996) Effects of castration on the carcass characteristics of Hanwoo and Holstein. J. Anim. Sci. Technol. (Kor.). 38, 239-248.

12.
Kim, D. G., Jung, K. K., Sung, S. K., Choi, C. B., Kim, S. K., Kim, D. Y., and Choi, B. K. (1996) Effects of age in the carcass characteristics of Hanwoo and Holstein steers. J. Anim. Sci. Technol. (Kor.) 38, 268-274.

13.
Kim, K. H., Lee, J. H., Oh, Y. G., Kang, S. W., Lee, S. C., Park, W. Y., and Ko, Y. D. (2005) The optimal TDN levels of concentrates and slaughter age in Hanwoo steers. J. Anim. Sci. Technol. (Kor.). 47, 731-744. crossref(new window)

14.
Kramer, A., Stigler, R., Knaute, T., Hoffmann, B., and Schneider-Mergener, J. (1998) Stepwise transformation of a cholera toxin and a p24(HIV-1) epitope into D-peptide analogs. Protein. Eng. 11, 941-948. crossref(new window)

15.
Lee, K. C., Park, N. H., Jeong, J., Lee, S. S., Oh, Y. S., Baek, K. H., Jung, K. K., and Choi, C. B. (2004) Effects of castration and slaughtering ages on Physico-chemical characteristics of Hanwoo M. Longissimus dorsi. J. Anim. Sci. Technol. (Kor.). 46, 165-172. crossref(new window)

16.
Lee, K. J. and Watson, R. D. (2002) Antipeptide antibodies for detecting crab (Callinectes sapidus) molt-inhibiting hormone. Peptides 23, 853-862. crossref(new window)

17.
Lee, M. Y. (1998) Effects of body weight and age on carcass traits of Hanwoo (Korean cattle) steer. J. Anim. Sci. Technol. (Kor.). 40, 221-226.

18.
Lee, S. S., Jung, J. K., Park, N. H., and Won, Y. S. (1997) Effects of castration on the carcass characteristics and serum metabolites in Korean cattle. J. Anim. Sci. Technol. (Kor.). 39, 145-154.

19.
Macedo-Silva, A., Barbosa, S. F. C., Alkmin, M. G. A., Vaz, A. J., Shimokomaki, M., and Tenuta, A. A. (2000) Hamburger meat identification by dot-ELISA. Meat Sci. 56, 189-192. crossref(new window)

20.
Mohri, H., Asakura, Y., Fukushima, J., Kawamoto, S., Okubo, T., and Okuda, K. (1997) Synthetic peptide from the V3 loop consensus motif with a potent anti-HIV activity inhibits ristocetin- mediated vWF-GPIb interaction. Peptides 18, 1289-1293. crossref(new window)

21.
Nargi, F., Kramer, E., Mezencio, J., Whetstone, C., Van Regenmortel, M. H. V., Briand, J. P., Muller, S., and Brown, F. (1999) Protection of swine from foot-and-mouth disease with one dose of an all-D retro peptide. Vaccine 17, 2888-2893. crossref(new window)

22.
Paek, B. H., Kang, S. W., Cho, Y. M., Cho, W. M., Yang, C. J., and Yun, S. G. (2005) Effects of substituting concentrates with dried leftover food on growth and carcass characteristics of Hanwoo steers. Asian-Aust. J. Anim. Sci. 18, 209-213. crossref(new window)

23.
Song, M. K. and Choi, Y. I. (1994) Effect of feeding method, supplementation of Yellow Grease and duration of feeding on carcass characteristics and meat quality of Korean native bulls. Korean J. Anim. Nutr. Feed. 18, 30-37.

24.
Song, M. K., Kim, N. S., Chung, C. S., Choi, Y. I., Won, Y. S., Chung, J. K., and Choi, S. H. (1998) Effect of feeding level of concentrates on growth performance and fatty acid composition of adipose tissues at various locations in Hanwoo bulls. J. Anim. Sci. Technol. (Kor.). 40, 485-498.

25.
Sung, S. K., Jung, K. K., Choi, C. B., Kim, D. G., Kim, S. G., Kim, D. Y., and Choi, B. J. (1996) Effects of castration and age on the carcass composition and retail yields of Hanwoo and Holstein. J. Anim. Sci. Technol. (Kor.). 38, 261-267.

26.
Tooyama, I., Abe, H., Renda, T. G., and Kimura, H. (2000) Production and immunohistochemical application of antiserum against Tyr-D-Ala-Phe, a N-terminal tripeptide common to dermorphin/deltorphin family. Peptides 21, 1649-1655. crossref(new window)

27.
Van Rgenmortel, M. H. V. and Muller, S. (1998) D-peptides as immunogens and diagnostic reagents. Curr. Opin. Biotechnol. 9, 377-382. crossref(new window)