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Alteration of Porcine Serum Albumin Levels in Pork Meat by Marination in Kiwi or Pineapple Juice and Subsequent Pan Broiling
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 Title & Authors
Alteration of Porcine Serum Albumin Levels in Pork Meat by Marination in Kiwi or Pineapple Juice and Subsequent Pan Broiling
Moon, Sung-Sil; Kim, Dongwook; Kim, Il-Suk; Ham, Jun-Sang; Park, Beom-Young; Jang, Aera;
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This study was conducted to evaluate the changes in porcine serum albumin (PSA), a major allergen, which occur when raw pork ham is marinated with kiwi or pineapple juice, and/or when the ham is pan broiled at for 4 min after marination. In this study, raw pork ham was soaked for 4 h or 8 h in marinades containing commercial marinating sauce only, commercial marinating sauce and 7% kiwi juice, or commercial marinating sauce and 7% pineapple juice. When the meat was marinated and then pan-broiled, pork ham meat protein was significantly denatured and hydrolyzed, and the level of PSA in the meat was significantly reduced. The PSA contents of pork broiled without marination, pork that had been marinated in commercial marinating sauce alone, pork that had been marinated in commercial marinating sauce with kiwi juice, and pork that had been marinated in commercial marinating sauce with pineapple juice, were 95.4, 43.3, 14.3, and 5.4 ng/mL, respectively (p<0.05). Marinating with pineapple juice was more effective than marinating with kiwi juice; and marination for 8 h was more effective than marinating for 4 h. These results indicate that the level of PSA in pork ham is effectively reduced, when the meat is first marinated in sauces that contain kiwi or pineapple extracts for 8 h, rather than 4 h, and then cooked. Further study is needed to determine whether marinated pork meat reduces allergenicity in vivo, as well.
porcine serum albumin;marinating;allergen;sandwich ELISA;pork ham;
 Cited by
Besler, M., Steinhart, H., and Paschke, A. (2001) Stability of food allergens and allergenicity of processed foods. J. Chrom. B 756, 207-228. crossref(new window)

Chen, X., Feng, B. S., Zheng, P. Y., Liao, X. Q., Chong, J., Tang, S. G., and Yang, P. C. (2008) Fc gamma receptor signaling in mast cells links microbial stimulation to mucosal immune inflammation in the intestine. Am. J. Pathol. 173, 1647-1656. crossref(new window)

Cho, E. D., Kim, D. S., and Jung, K. H. (2001) Identification of the chicken meat allergens. J. App. Pharm. 9, 7-14.

Chung, H. J., Park, J. H., Kim, J. H., Kim, Y. O., Chung, S.T., Kim, J. H., Cho, E. D., Cho, D. H., Noh, G. W., and Kim, D. S. (2001) Identification of allergens in pork meat. J. Pharm. Korea 45, 39-45.

El-Gharbawi, M. and Witaker, J. R. (1963) Factors affecting enzymatic solubilization of beef proteins. J. Food Sci. 28, 168-172. crossref(new window)

Fiocchi, A., Restani, P., and Riva, E. (2000) Beef allergy in children. Nutrition 16, 454-457. crossref(new window)

Fisher, A. A. (1982) Allergic contact urticaria to raw beef: Hisopathology of the specific wheal reaction at the scratch test site. Contact Derm. 8, 425-425. crossref(new window)

Ha, M., Bekhit, A. E. A., Carne, A., and Hopkins, D. L. (2012) Characterisation of commercial papain, bromelain, actinidin and zingibain protease preparations and their activities toward meat proteins. Food Chem. 134, 95-105. crossref(new window)

Kang, C. K. and Rice, E. E. (1970) Degradation of various meat fractions by tenderizing enzymes. J. Food Sci. 35, 563-565. crossref(new window)

Kim, H. and Taub, I. A. (1991) Specific degradation of myosin in meat by bromelain. Food Chem. 40, 337-343. crossref(new window)

Kim, K. B. W. R., Song, E. J., Lee, S. Y., Park, J. G., Lee, J. W., Byun, M. W., and Ahn, D. H. (2011) Changes in antigenicity of porcine serum albumin in gamma-irradiated sausage extract by treatment with pepsin and trypsin. Radiat. Phys. Chem. 80, 1258-1262. crossref(new window)

Kim, M. H., Rho, J., and Kim, M. J. (2010) Proteolytic effect of fruit lesh and crude enzyme extract from fruits on myofibrilar protein. Korean J. Food Cookery Sci. 26, 323-329.

Korea Meat Trade Association (2013) Statistics of meat consumption. Available from: Accessed Jan. 23, 2014.

Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685. crossref(new window)

Lee, S. I. (1993) The role of IgG in allergic disease. Pediatr. Allergy Respir. Dis. (Korea). 3, 3-10.

Sakai, Y., Yamato, R., Onuma, M., Kikuta, T., Watanabe, M., and Nakayama, T. (1998) Non-antigenic and low allergic gelatin produced by specific digestion with an enzyme-coupled matrix. Biol. Pharm. Bull. 21, 330-334. crossref(new window)

Sicherer, S. H. and Sampson, H. A. (2010) Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment. J. Allergy Clin. Immunol. 125, S116-S125. crossref(new window)

Tappel, A. L., Miyada, D. S., Sterling, C., and Maier, V. P. (1956) Meat tenderization. II. Factors affecting the tenderization of beef by papain. Food Res. 21, 375-383. crossref(new window)

Wang, C. H., Kou, S. K., and Chen, H. L. (2002) Porcine serum albumins sandwith ELISA for determining the cooking temperature of cured ground pork. Taiwanese J. Agric. Food Chem. 40, 197-204.

Wang, H., Weir, C. E., Birkner, M. L., and Ginger, B. (1958) Studies on enzymatic tenderization of meat. III. Histological and panel analyses of enzyme preparations from three distinct sources. Food Res. 23, 423-438. crossref(new window)