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Effects of Psychrotrophic Bacteria, Serratia liquefaciens and Acinetobacter genomospecies 10 on Yogurt Quality
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 Title & Authors
Effects of Psychrotrophic Bacteria, Serratia liquefaciens and Acinetobacter genomospecies 10 on Yogurt Quality
Shin, Yong Kook; Oh, Nam Su; Lee, Hyun Ah; Choi, Jong-Woo; Nam, Myoung Soo;
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The aim of this study was to evaluate the effect of proteolytic (Serratia liquefaciens, match %: 99.39) or lipolytic (Acinetobacter genomospecies 10, match %: 99.90) psychrotrophic bacteria (bacterial counts, analysis of free fatty acids (FFA) and analysis of free amino acids) on the microbial and chemical properties (yogurt composition), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of yogurt during storage. Yogurts were prepared with raw milk preinoculated with each psychrotrophic bacteria. The total solid, fat, and protein content were not affected by preinoculation, but the pH of yogurt preinoculated with psychrotrophic bacteria was higher than in control. There was a dramatic increase in short chain free fatty acids among FFA in yogurt with Acinetobacter genomospecies 10. For 14 d of cold storage condition, SCFFA was 25.3 mg/kg to 34.4 mg/kg (1.36 times increased), MCFFA was 20.4 mg/kg to 25.7 mg/kg (1.26 times increased), and LCFFA was 240.2 mg/kg to 322.8 mg/kg (1.34 times increased). Serratia liquefaciens (match %: 99.39) in yogurt caused a greater accumulation of free amino acids (FAA), especially bitter peptides such as leucine, valine, arginine, and tyrosine, but SDS-PAGE showed that the inoculation of Serratia liquefaciens did not affect the degree of casein degradation during storage. Taken together, the excessive peptides and FFA in yogurt generated from psychrotrophic bacteria could develop off-flavors that degrade the quality of commercial yogurt products.
psychrotrophic bacteria;Serratia liquefaciens;Acinetobacter genomospecies 10;free fatty acids;free amino acids;
 Cited by
Extended storage of cold raw milk on yogurt manufacturing, Pesquisa Agropecuária Brasileira, 2017, 52, 2, 104  crossref(new windwow)
Adams, D., Barach, J., and Speck, M. (1976) Effect of psychrotrophic bacteria from raw milk on milk proteins and stability of milk proteins to ultrahigh temperature treatment. J. Dairy Sci. 59, 823-827. crossref(new window)

AOAC (2007) Official methods of analysis. 18th ed, Association of Official Analytical Chemists, Washington DC, pp. 68-81.

Bigalke, D. (1985) Lipolytic and proteolytic microorganisms and their enzymes. II. Dairy and Food Sanitation 5, 388-389.

Cousin, M. (1982) Presence and activity of psychrotrophic microorganisms in milk and dairy products: a review. J. Food Prot. 45, 172-207.

Cousin, M. and Marth, E. (1977) Cheddar cheese made from milk that was precultured with psychrotrophic bacteria. J. Dairy Sci. 60, 1048-1056. crossref(new window)

De Jong, C. and Badings, H. T. (1990) Determination of free fatty acids in milk and cheese procedures for extraction, clean up, and capillary gas chromatographic analysis. J. High Resolut. Chromatogr. 13, 94-98. crossref(new window)

Deeth, H. (2002) Lipolysis in milk and dairy products: a research journey. Food Aust. 54, 433-436.

Dumont, J., Delespaul, G., Miguot, B., and Adda, J. (1977) Influence des bacteries psychrotrophes sur les qualites organoleptiques de fromages a pate molle. Le Lait 57, 619-630. crossref(new window)

Hong, J. L. (1994) Determination of amino acids by precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate and high performance liquid chromatography with ultraviolet detection. J. Chromatogr. A. 670, 59-66. crossref(new window)

Ingraham, J. (1962) Temperature relationships. The Bacteria 4, 265-296.

Ingraham, J. and Stokes, J. (1959) Psychrophilic bacteria. Bacteriol. Rev. 23, 97-108.

Iwasaki, T. and Kosikowski, F. V. (1973) Increasing flavor in cheese with commercial microbial enzyme preparations. J. Dairy Sci. 56, 623-624.

Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685. crossref(new window)

Law, B., Sharpe, M. E., and Chapman, H. R. (1976) The effect of lipolytic Gram-negative psychrotrophs in stored milk on the development of rancidity in Cheddar cheese. J. Dairy Res. 43, 459-468. crossref(new window)

Mikolajcik, E. (1979) Psychrotrophic bacteria and dairy product quality. 1. Major organisms involved and defects produced. Cult. Dairy Prod. J. 14, 6-10.

Pinheiro, A., Liska, B., and Parmelee, C. (1965) Heat stability of lipases of selected psychrophilic bacteria in milk and Purdue Swiss-type cheese. J. Dairy Sci. 48, 983-984. crossref(new window)

Reiter, B., Fryer, T., Pickering, A., Chapman, H. R., Lawrence, R., and Sharpe, M. E. (1967) The effect of the microbial flora on the flavour and free fatty acid composition of Cheddar cheese. J. Dairy Res. 34, 257-272. crossref(new window)

Serra, M., Trujillo, A., Pereda, J., Guamis, B., and Ferragut, V. (2008) Quantification of lipolysis and lipid oxidation during cold storage of yogurts produced from milk treated by ultra-high pressure homogenization. J. Food Eng. 89, 99-104. crossref(new window)

Shelley, A., Deeth, H., and Macrae, I. (1986) Growth of lipolytic psychrotrophic pseudomonads in raw and ultra-heattreated milk. J. Appl. Microbiol. 61, 395-400.

Shin, Y. K., Oh, N. S., Lee, H. A., and Nam, M. S. (2013) Effects of psychrotrophic bacteria Acinetobacter genomospecies 10 and Serratia liquefaciens on raw milk quality. Korean J. Food Sci. An. 33, 542-548. crossref(new window)

Sorhaug, T. and Stepaniak, L. (1997) Psychrotrophs and their enzymes in milk and dairy products: quality aspects. Trends Food Sci. Tech. 8, 35-41. crossref(new window)

Stead, D. (1986) Microbial lipases: their characteristics, role in food spoilage and industrial uses. J. Dairy Res. 53, 481-505. crossref(new window)

Stokes, J. (1963) In Recent Progress in Microbiology, Canada, University of Toronto Press, 8, 187-192.

Thomas, S. (1966) Sources, incidence and significance of psychrotrophic bacteria in milk. Milchwissenschaft 21, 270-275.

Thomas, S. and Thomas, B. F. (1973) Psychrotrophic bacteria in refrigerated bulk-collected raw milk. Part I. Dairy Ind. 38, 11-15.

Wessels, D., Jooste, P. J., and Mostert, J. F. (1988) The occurrence and importance of enterobacteriaceae isolates in milk and dairy products. S. Afr. J. Dairy Sci. 20, 23-28.

White, C. and Marshall, R. (1973) Reduction of shelf-life of dairy products by a heat-stable protease from Pseudomonas fluorescens P26. J. Dairy Sci. 56, 849-853. crossref(new window)

Witter, L. D. (1961) Psychrophilic bacteria-a review. J. Dairy Sci. 44, 983-1015. crossref(new window)