Advanced SearchSearch Tips
Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray
Kim, Mi-Jung; Lee, Ju-Woon; Sung, Nak-Yoon; Kim, Su-Min; Hwang, Young-Jung; Kim, Jae-Hun; Song, Beom-Seok;
  PDF(new window)
This study was conducted to compare the effects of two forms of radiation (electron and X-ray; generated by an electron beam accelerator) on the conformation and antigenic properties of hen`s egg albumin, ovalbumin (OVA), which was used as a model protein. OVA solutions (2.0 mg/mL) were individually irradiated by electron beam or X-ray at the absorbed doses of 0 (control), 2, 4, 6, 8, and 10 kGy. No differences between the two forms of radiation on the structural properties of OVA were shown by spectrometric and electrophoretic analyses. The turbidity of OVA solution increased and the main OVA bands on polyacrylamide gels disappeared after irradiation, regardless of the radiation source. In competitive indirect enzyme-linked immunosorbent assay, OVA samples irradiated by electron beam or X-ray showed different immunological responses in reactions with monoclonal and polyclonal antibodies (immunoglobulin G) produced against non-irradiated OVA. The results indicate that electron beam irradiation and X-ray irradiation produced different patterns of structural changes to the OVA molecule.
electron beam;X-ray;ovalbumin;conformational change;
 Cited by
Al-kahtani, H. A., Abu-Tarbouch, H. M., Atia, M., Bajaber, A. S., Ahmed, M. A., and El-Mojaddidi, M. A. (1998) Amino acid and protein changes in tilapia and Spanish mackerel after irradiation and storage. Radiat. Phys. Chem. 51, 107-114. crossref(new window)

Byun, M. W., Kim, J. H., Lee, J. W., Park, J. W., Hong, C. S. and Kang, I. J. (2000) Effects of gamma irradiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp. J. Food Prot. 63, 940-944.

Byun, M. W., Oh, S. H., Kim, J. H., Yoon, Y. H., Park, S. C., Kim, H. S., Kim, S. B., Han, S. B., and Lee, J. W. (2009) Information channel effects on women intention to purchase irradiated food in Korea. Radiat. Phys. Chem. 78, 675-677. crossref(new window)

Chan, J. K., Gill, T. A., and Paulson, A. T. (1993) Thermal aggregation of myosin subfragments from cod and herring. J. Food Sci. 58, 1057-1061. crossref(new window)

Cho, K. H., Yook, H. S., Lee, J. W., Lee, S. Y., and Byun, M. W. (2001) Changes of binding ability of milk-hypersensitive patients' IgE to gamma-irradiated milk proteins. J. Korean Soc. Food Sci. Nutr. 30, 505-509.

Cleland, M. R. and Stichelbaut, F. (2013). Radiation processing with high-energy X-rays. Radiat. Phys. Chem. 84, 91-99. crossref(new window)

Filali-Mouthim, A., Audette, M., St-louis, M., Thauvett, L., Denoroy, L., Penin, F., Chen, X., Rouleau, N., Le Caer, J. P., Rossier, J., Potier, M., and Le Maire, M. (1997) Lysozyme fragmentation induced by gamma-radiolysis. Int. J. Radiat. Biol. 72, 63-71. crossref(new window)

Hill, H. D. and Straka, J. G. (1988) Protein determination using bicinchoninic acid in the presence of sulfhydryl reagents. Anal. Biochem. 170, 203-208. crossref(new window)

Kang, K. O., Lee, J. W., Jo, C. R., Yook H. S., and Byun, M. W. (2002) Changes of allergenicity and conformational structure of egg ovomucoid by gamma irradiation in the basic condition. J. Food Sci. Nut. 7, 52-56. crossref(new window)

Kim, M. J., Lee, J. W., Yook, H. S., Lee, S. Y., Kim, M. C., and Byun, M. W. (2002) Changes in the antigenic and immunoglobulin E-binding properties of hen's egg albumin with the combination of heat and gamma irradiation treatment. J. Food Prot. 65, 1192-1195.

Kume, T., Ishii, T., and Matsuda, T. (1994) Immunochemical identification of irradiated chicken eggs. J. Sci. Food Agric. 65, 1-4. crossref(new window)

Kume, T. and Matsuda, T. (1995) Changes in structural and antigenic properties of proteins by radiation. Radiat. Phys. Chem. 46, 225-231. crossref(new window)

Kuntz, E. and White, E. (1961) Effects of electron beam irradiation on collagen. Federation Pro. 20, 376-391.

Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685. crossref(new window)

Lee, J. W., Kim, J. H., Yook, H. S., Kang, K. O., Lee, S. Y., Hwang, H. J., and Byun, M. W. (2000a) Effects of gamma radiation on the allergenic and antigenic properties of milk proteins. J. Food Prot. 64, 272-276.

Lee, J. W., Yook, H. S., Lee, K. H., Kim, J. H., Kim, W. J., and Byun, M. W. (2000b) Conformational changes of myosin by gamma irradiation. Radiat. Phys. Chem. 58, 271-277. crossref(new window)

Lee, J. W., Yook, H. S., Cho, K. H., Lee, S. Y., and Byun, M. W. (2001) The changes of allergenic and antigenic properties of egg white albumin (Gal d 2) by gamma irradiation. J. Korean Soc. Food Sci. Nutr. 30, 500-504.

Lee, J. W. and Byun, M. W. (2003) Food irradiation technology and structural changes of food allergens. J. Asth. Aller. Clin. Immunol. 23, 16-23.

Miller, R. B. (2003) Food irradiation using bremsstrahlung X-rays. Radiat. Phys. Chem. 68, 963-974. crossref(new window)

Palou, L., Marcilla, A., Rojas-Argudo, C., Alonso, M., Jacas, J. A., and del Rio, M. A. (2007) Effects of X-ray irradiation and sodium carbonate treatments on postharvert Penicillium decay and quality attributes of clementine mandarins. Post. Bio. Technol. 46, 252-261. crossref(new window)

SAS (2008) SAS/STAT Software for PC. Release 9.2, SAS Institute Inc., Cary, NC, USA.

Seo, J. H., Kim, J. H., Lee, J. W., Yoo, Y. C., Kim, M. R., Park, K. S., and byun, M. W. (2007) Ovalbumin modified by gamma irradiation alters its immunological functions and allergic responses. Inter. Immunopharm. 7, 464-472. crossref(new window)

Thayer, D. W. (1994) Wholesomeness of irradiated foods. Food Technol. 48, 58-67.

Van Calenberg, S., Van Cleempu, O., Mondelaers, W., and Huyghebaert, A. (1999) Comparison of the effect of X-ray and electron beam irradiation on the microbiological quality of foodstuffs. LWT-Food Sci. Technol. 32, 372-376. crossref(new window)

Van Calenberg, S., Vanhaelewyn, G., Van Cleemput, O., Callens, F., Mondelaers, W., and Huyghebaert, A. (1998) Comparison of the effect of X-ray and electron beam irradiation on some selected spices. LWT-Food Sci. Technol. 31, 252-258. crossref(new window)