Advanced SearchSearch Tips
Analysis of Myosin Heavy Chain Isoforms from Longissimus Thoracis Muscle of Hanwoo Steer by Electrophoresis and LC-MS/MS
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Analysis of Myosin Heavy Chain Isoforms from Longissimus Thoracis Muscle of Hanwoo Steer by Electrophoresis and LC-MS/MS
Kim, Gap-Don;
  PDF(new window)
The purpose of this study was to analyze myosin heavy chain (MHC) isoforms in bovine longissimus thoracis (LT) muscle by liquid chromatography (LC) and mass spectrometry (MS). LT muscles taken from Hanwoo (Korean native cattle) steer (n=3) used to separate myosin bands by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The peptide queries were obtained from the myosin bands by LC-MS/MS analysis following in-gel digestion with trypsin. A total of 33 and 43 queries were identified as common and unique peptides, respectively, of MHC isoforms (individual ions scores >43 indicate identity or extensive homology, p<0.05). MHC-1 (IIx), -2 (IIa), -4 (IIb), and -7 (slow/I) were identified based on the Mowse score (5118, 3951, 2526, and 2541 for MHC-1, -2, -4, and -7, respectively). However, more analysis is needed to confirm the expression of MHC-4 in bovine LT muscle because any query identified as a unique peptide of MHC-4 was not found. The queries that were identified as unique peptides could be used as peptide markers to confirm MHC-1 (14 queries), -2 (8 queries), and -7 (21 queries) in bovine LT muscle; no query identified as a unique peptide of MHC-4 was found. LC-MS/MS analysis is a useful approach to study MHC isoforms at the protein level.
myosin heavy chain;LC-MS/MS;cattle;longissimus thoracis;
 Cited by
Analysis of Methionine Oxidation in Myosin Isoforms in Porcine Skeletal Muscle by LC-MS/MS Analysis, Korean Journal for Food Science of Animal Resources, 2016, 36, 2, 254  crossref(new windwow)
Comparison of Characteristics of Myosin Heavy Chain-based Fiber and Meat Quality among Four Bovine Skeletal Muscles, Korean Journal for Food Science of Animal Resources, 2016, 36, 6, 819  crossref(new windwow)
Abreu, E., Quirox-Rothe, E., Mayoral, A. I., Vivo, J. M., Robina, A., Guilen, M. T., Aguera, E., and Rivero, J. L. (2006) Myosin heavy chain fibre types and fibre sizes in nulliparous and primiparous ovariectomized Iberian sows: Interaction with two alternative rearing systems during the fattening period. Meat Sci. 74, 359-372. crossref(new window)

Acevedo, L. M. and Rivero, J. L. (2006) New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes. Cell Tissue Res. 323, 283-303. crossref(new window)

Berg, J. S., Powell, B. C., and Cheney, S. E. 2001. A millennium myosin census, Mol. Biol. Cell 12, 780-794. crossref(new window)

Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. crossref(new window)

Chikuni, K., Muroya, S., and Nakajima, I. (2004) Myosin heavy chains expressed in bovine skeletal muscles. Meat Sci. 67, 87-94. crossref(new window)

Graziotti, G. H., Ríos, C. M., and Rivero, J. L. L. (2001) Evidence for three fast myosin heavy chain isoforms in type II skeletal muscle fibers in adult llama (Lama glama). J. Histochem. Cytochem. 49, 1033-1044. crossref(new window)

Kim, G. D., Jeong, J. Y., Yang, H. S., and Joo, S. T. (2013a) Identification of myosin heavy chain isoforms in porcine longissimus dorsi muscle by electrophoresis and mass spectrometry. Electrophoresis 34, 1255-1261. crossref(new window)

Kim, G. D., Ryu, Y. C., Jeong, J. Y., Yang, H. S., and Joo, S. T. (2013b) Relationship between pork quality and characteristics of muscle fibers classified by the distribution of myosin heavy chain isoforms. J. Anim. Sci. 91, 5525-5534. crossref(new window)

Kim, G. D., Ryu, Y. C., Jo, C., Lee, J. G., Yang, H. S., Jeong, J. Y., and Joo, S. T. (2014) The characteristics of myosin heavy chain-based fiber types in porcine longissimus dorsi muscle. Meat Sci. 96, 712-718. crossref(new window)

Kim, J. S., Hinchcliff, K. W., Yamaguchi, M., Beard, L. A., Markert, C. D., and Devor, S. T. (2005) Age-related changes in metabolic properties of equine skeletal muscle associated with muscle plasticity. Vet. J. 169, 397-403. crossref(new window)

Kjellgren, D., Thornell, L. E., Andersen, J., and Pedrosa-Domellof, F. (2003) Myosin heavy chain isoforms in human extraocular muscles. Invest. Ophthalmol. Vis. Sci. 44, 1419-1425. crossref(new window)

Kohn, T. A., Hoffman, L. C., and Myburgh, K. H. (2007) Identification of myosin heavy chain isoforms in skeletal muscle of four Southern African wild ruminants. Comp. Biochem. Physiol. A Physiol. Part A 148, 399-407. crossref(new window)

Lefaucheur, L., Ecolan, P., Plantard, L., and Gueguen, N. (2002) New insights into muscle fiber types in the pig. J. Histochem. Cytochem. 50, 719-730. crossref(new window)

Lefaucheur, L., Milan, D., Ecolan, P., and Le Callennec, C. (2004) Myosin heavy chain composition of different skeletal muscles in Large White and Meishan pigs. J. Anim. Sci. 82, 1931-1941.

Pette, D. and Staron, R. S. (2000) Myosin isoforms, muscle fiber types, and transitions. Microsc. Res. Tech. 50, 500-509. crossref(new window)

Picard, B., Barboiron, C., Duris, M. P., Gagniere, H., Jurie, C., and Geay, Y. (1999) Electrophoretic separation of bovine muscle myosin heavy chain isoforms. Meat Sci. 53, 1-7. crossref(new window)

Picard, B. and Cassar-Malek, I. (2009) Evidence for expression of IIb myosin heavy chain isoform in some skeletal muscles of Blonde d'Aquitaine bulls. Meat Sci. 82, 30-36. crossref(new window)

Qin, H., Hsu, M. K. H., Morris, B. J., and Hoh, J. F. Y. (2002) A distinct subclass of mammalian striated myosins: structure and molecular evolution of 'superfast' or masticatory myosin heavy chain, J. Mol. Evol. 55, 544-552. crossref(new window)

Rayment, I., Rypniewski, W. R., Schmidt-Base, K., Smith, R., Tomchick, D. R., Benning, M. M., Winkelmann, D. A., Wesenberg, G., and Holden, H. M. (1993) Three dimensional structure of myosin subfragment-1: a molecular motor. Science 261, 50-58. crossref(new window)

Talmadge, R. J. and Roy, R. R. (1993) Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms. J. Appl. Physiol. 75, 2337-2340.

Tokunaga, M., Sutoh, K., Toyoshima, C., and Wayabashi, T. (1987) Location of the ATPase site of myosin determined by three-dimensional electron microscopy. Nature 329, 635-638. crossref(new window)

Weiss, A., Schiaffino, S., and Leinwand, L. A. (1999) Comparative sequence analysis of the complete human sarcomeric myosin heavy chain family: implications for functional diversity. J. Mol. Biol. 290, 61-75. crossref(new window)